LAMP1 Antibody - #DF7033

Fig. 5| ABHD5 sustains the activity of RNASET2. a Representative images of immunofluorescent stainings of lysosome (stained by Lysotracker, red) and ABHD5 (green) in SW480 cells. Scale bar: 10 μm. b Western blots showing ABHD5 expression in the lysosome lysates of ABHD5 KD and control cells. LAMP1 was used as a reference.

Figure 2. SFTSV-induced autophagy is a complete process. (A) Vero or HeLa cells were mock infected or infected with SFTSV at MOI of 5 for 24 h and 36 h and then treated with CQ for 6 h. Cell lysates were evaluated via WB. (B) Vero or HeLa cells were mock infected or infected with SFTSV for 24 h and then treated with Baf-A1 for 6 h. Cell lysates were evaluated via WB. (C) Vero cells were transfected with mCherry-GFP-LC3 for 12 h and then were mock infected or infected with SFTSV, or treated with CQ (100 µM), or starved in EBSS medium for 6 h. The number of mcherry-GFP-LC3 dots in each cell was counted, and at least 20 cells were included for each group. Single plane type of images was present. Scale bar: 20 μm. (D) Vero cells were infected with SFTSV for 24 h for analyzing the colocalization of endogenous LC3 and LAMP1. Single plane type of images was present. Scale bar: 20 μm.

FIGURE 6 Effects of N2FBR on lysosome injury. (A) Protein levels of LAMP1 and Legumain as determined by western blotting. Relative density values showing (B) LAMP1 and (C) Legumain expression. (D) Mean immunofluorescence intensity of TFEB. (E) Representative images of immunofluorescence showing the expression of TFEB. (F) Representative images of immunohistochemical staining for Legumain. Data are expressed as *p < 0.05, **p < 0.01, compared with sham group; #p < 0.05, ##p < 0.01, compared with model group.

Figure 3. |CTRP3 directly interacted with LAMP1. (A, B) Relative mRNA and protein expression of CTRP3 was determined by using RT‐qPCR and Western blottin

Figure 3 CTRP3 directly interacted with LAMP1. (A, B) Relative mRNA and protein expression of CTRP3 was determined by using RT-qPCR and Western blotting. (C) The relationship between CTRP3 and LAMP1 was detected by using Co-IP. (D) The effects of CTRP3 silence or overexpression on the mRNA level of LAMP1 was measured by using RT-qPCR.

Figure 2 Effects of CA on oxidative stress. Total serum levels of (a) MDA, (b) SOD, and (c) TAC as measured through ELISA. (d) Relative mRNA expression of Nrf2. (e) Protein levels of HO-1. (f) Relative density values of HO-1 expression. ∗∗P < 0.01, compared with the NC group, #P < 0.05, ##P < 0.01, compared with the BLM group.