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产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF7041, RRID:AB_2838997.
展开/折叠
CI-75kD; Complex I 75Kd; Complex I, mitochondrial respiratory chain, 75 kD subunit; Complex I-75kD; mitochondrial; NADH coenzyme Q reductase; NADH dehydrogenase (ubiquinone) FeS protein 1 (75kD) (NADH coenzyme Q reductase); NADH ubiquinone oxidoreductase 75 kDa subunit mitochondrial; NADH-ubiquinone oxidoreductase 75 kDa subunit; NDUFS1; NDUS1_HUMAN; PRO1304;
抗原和靶标
- P28331 NDUS1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MLRIPVRKALVGLSKSPKGCVRTTATAASNLIEVFVDGQSVMVEPGTTVLQACEKVGMQIPRFCYHERLSVAGNCRMCLVEIEKAPKVVAACAMPVMKGWNILTNSEKSKKAREGVMEFLLANHPLDCPICDQGGECDLQDQSMMFGNDRSRFLEGKRAVEDKNIGPLVKTIMTRCIQCTRCIRFASEIAGVDDLGTTGRGNDMQVGTYIEKMFMSELSGNIIDICPVGALTSKPYAFTARPWETRKTESIDVMDAVGSNIVVSTRTGEVMRILPRMHEDINEEWISDKTRFAYDGLKRQRLTEPMVRNEKGLLTYTSWEDALSRVAGMLQSFQGKDVAAIAGGLVDAEALVALKDLLNRVDSDTLCTEEVFPTAGAGTDLRSNYLLNTTIAGVEEADVVLLVGTNPRFEAPLFNARIRKSWLHNDLKVALIGSPVDLTYTYDHLGDSPKILQDIASGSHPFSQVLKEAKKPMVVLGSSALQRNDGAAILAAVSSIAQKIRMTSGVTGDWKVMNILHRIASQVAALDLGYKPGVEAIRKNPPKVLFLLGADGGCITRQDLPKDCFIIYQGHHGDVGAPIADVILPGAAYTEKSATYVNTEGRAQQTKVAVTPPGLAREDWKIIRALSEIAGMTLPYDTLDQVRNRLEEVSPNLVRYDDIEGANYFQQANELSKLVNQQLLADPLVPPQLTIKDFYMTDSISRASQTMAKCVKAVTEGAQAVEEPSIC
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P28331 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S14 | Phosphorylation | Uniprot | |
| K15 | Ubiquitination | Uniprot | |
| S16 | Phosphorylation | Uniprot | |
| K84 | Acetylation | Uniprot | |
| K108 | Ubiquitination | Uniprot | |
| K157 | Methylation | Uniprot | |
| K163 | Ubiquitination | Uniprot | |
| T239 | Phosphorylation | Uniprot | |
| K289 | Acetylation | Uniprot | |
| K289 | Ubiquitination | Uniprot | |
| K298 | Methylation | Uniprot | |
| K298 | Ubiquitination | Uniprot | |
| K311 | Acetylation | Uniprot | |
| K311 | Ubiquitination | Uniprot | |
| Y316 | Phosphorylation | Uniprot | |
| T317 | Phosphorylation | Uniprot | |
| K420 | Ubiquitination | Uniprot | |
| S434 | Phosphorylation | Uniprot | |
| T439 | Phosphorylation | Uniprot | |
| Y440 | Phosphorylation | Uniprot | |
| T441 | Phosphorylation | Uniprot | |
| S448 | Phosphorylation | Uniprot | |
| K450 | Ubiquitination | Uniprot | |
| K467 | Acetylation | Uniprot | |
| K467 | Ubiquitination | Uniprot | |
| K470 | Ubiquitination | Uniprot | |
| K471 | Ubiquitination | Uniprot | |
| S478 | Phosphorylation | Uniprot | |
| S479 | Phosphorylation | Uniprot | |
| K499 | Acetylation | Uniprot | |
| K499 | Ubiquitination | Uniprot | |
| T503 | Phosphorylation | Uniprot | |
| S504 | Phosphorylation | Uniprot | |
| T507 | Phosphorylation | Uniprot | |
| K531 | Ubiquitination | Uniprot | |
| K607 | Ubiquitination | Uniprot | |
| T611 | Phosphorylation | Uniprot | |
| K621 | Ubiquitination | Uniprot | |
| R624 | Methylation | Uniprot | |
| T697 | Phosphorylation | Uniprot | |
| K709 | Ubiquitination | Uniprot | |
| C710 | S-Nitrosylation | Uniprot | |
| C727 | S-Nitrosylation | Uniprot |
研究背景
Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized.
Mitochondrion inner membrane.
Complex I is composed of 45 different subunits.
Belongs to the complex I 75 kDa subunit family.
研究领域
· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).
· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.
· Human Diseases > Neurodegenerative diseases > Parkinson's disease.
· Human Diseases > Neurodegenerative diseases > Huntington's disease.
· Metabolism > Energy metabolism > Oxidative phosphorylation.
· Metabolism > Global and overview maps > Metabolic pathways.
· Organismal Systems > Nervous system > Retrograde endocannabinoid signaling. (View pathway)
文献引用
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