GNA11 Antibody - #DF7067

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产品: GNA11 抗体
货号: DF7067
描述: Rabbit polyclonal antibody to GNA11
应用: WB IHC
反应: Human, Mouse, Rat, Pig
预测: Pig, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
分子量: 42kDa; 42kD(Calculated).
蛋白号: P29992
RRID: AB_2839023

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 50ul RMB¥ 1250 现货
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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat,Pig
预测:
Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
GNA11 Antibody detects endogenous levels of total GNA11.
RRID:
AB_2839023
引用格式: Affinity Biosciences Cat# DF7067, RRID:AB_2839023.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

G alpha 11; G alpha q; G protein subunit alpha 11; GAQ; GNA 11; GNA11; GNAQ; Guanine nucleotide binding protein (G protein) alpha 11 (Gq class); Guanine nucleotide binding protein (G protein) alpha 11; Guanine nucleotide binding protein (G protein) q polypeptide; Guanine nucleotide binding protein alpha q; Guanine nucleotide binding protein G(q) subunit alpha; Guanine nucleotide binding protein G(y) subunit alpha; Guanine nucleotide binding protein Gq class GNA11; Guanine nucleotide binding protein subunit alpha 11;

抗原和靶标

免疫原:
Uniprot:

P29992(GNA11_HUMAN) >>Visit HPA database.

基因/基因ID:
GNA11(2767)
表达:
P29992 GNA11_HUMAN:

Expressed in testis.

描述:
Heterotrimeric guanine nucleotide-binding proteins (G proteins) consist of α, β and γ subunits and mediate the effects of hormones, neurotransmitters, chemokines and sensory stimuli. To date, over 20 known Gα subunits have been classified into four families, Gα(s), Gα(i/o), Gα(q) and Gα(12), based on structural and functional similarities (1,2). Phosphorylation of Tyr356 of Gα(q)/Gα(11) is essential for activation of the G protein, since phenylalanine substitution for Tyr356 changes the interaction of Gα with receptors and abolishes ligand-induced IP3 formation (3).
序列:
MTLESMMACCLSDEVKESKRINAEIEKQLRRDKRDARRELKLLLLGTGESGKSTFIKQMRIIHGAGYSEEDKRGFTKLVYQNIFTAMQAMIRAMETLKILYKYEQNKANALLIREVDVEKVTTFEHQYVSAIKTLWEDPGIQECYDRRREYQLSDSAKYYLTDVDRIATLGYLPTQQDVLRVRVPTTGIIEYPFDLENIIFRMVDVGGQRSERRKWIHCFENVTSIMFLVALSEYDQVLVESDNENRMEESKALFRTIITYPWFQNSSVILFLNKKDLLEDKILYSHLVDYFPEFDGPQRDAQAAREFILKMFVDLNPDSDKIIYSHFTCATDTENIRFVFAAVKDTILQLNLKEYNLV

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P29992 作为底物

Site PTM Type Enzyme
K16 Ubiquitination
R20 Methylation
K27 Ubiquitination
S50 Phosphorylation
K52 Ubiquitination
S68 Phosphorylation
K72 Ubiquitination
K98 Ubiquitination
K102 Sumoylation
K102 Ubiquitination
K107 Ubiquitination
K120 Ubiquitination
K133 Ubiquitination
C144 S-Nitrosylation
S154 Phosphorylation
K158 Ubiquitination
T162 Phosphorylation
T169 Phosphorylation
K252 Ubiquitination
K282 Ubiquitination
Y291 Phosphorylation
K345 Ubiquitination
K354 Ubiquitination

研究背景

功能:

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Acts as an activator of phospholipase C.

翻译修饰:

(Microbial infection) Deamidated at Gln-209 by Photorhabdus asymbiotica toxin PAU_02230, blocking GTP hydrolysis of heterotrimeric GNAQ or GNA11 and G-alphai (GNAI1, GNAI2 or GNAI3) proteins, thereby activating RhoA.

细胞定位:

Cell membrane>Lipid-anchor. Cytoplasm.
Note: In testicular cells, expressed exclusively in the cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in testis.

亚基结构:

G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Interacts with RGS22. Interacts with NTSR1.

蛋白家族:

Belongs to the G-alpha family. G(q) subfamily.

研究领域

· Cellular Processes > Cellular community - eukaryotes > Gap junction.   (View pathway)

· Environmental Information Processing > Signal transduction > Calcium signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Parasitic > Chagas disease (American trypanosomiasis).

· Human Diseases > Infectious diseases: Parasitic > Amoebiasis.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Organismal Systems > Circulatory system > Vascular smooth muscle contraction.   (View pathway)

· Organismal Systems > Nervous system > Cholinergic synapse.

· Organismal Systems > Nervous system > Long-term depression.

· Organismal Systems > Endocrine system > Insulin secretion.   (View pathway)

· Organismal Systems > Endocrine system > Aldosterone synthesis and secretion.

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