| 产品: | PAK4 抗体 |
| 货号: | DF7078 |
| 描述: | Rabbit polyclonal antibody to PAK4 |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog |
| 分子量: | 64kD; 64kD(Calculated). |
| 蛋白号: | O96013 |
| RRID: | AB_2839034 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF7078, RRID:AB_2839034.
展开/折叠
KIAA1142; p21 activated kinase 4; p21 Cdc42/Rac1-actiated kinase 4; P21 protein (Cdc42/Rac) activated kinase 4; p21(CDKN1A) activated kinase 4; p21-activated kinase 4; PAK 4; PAK-4; Pak4; PAK4_HUMAN; Protein kinase related to S.cerevisiae STE20 effector for Cdc42Hs; Serine threonine kinase PAK 4; Serine/threonine protein kinase PAK 4; Serine/threonine protein kinase PAK4; Serine/threonine-protein kinase PAK 4;
抗原和靶标
- O96013 PAK4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MFGKRKKRVEISAPSNFEHRVHTGFDQHEQKFTGLPRQWQSLIEESARRPKPLVDPACITSIQPGAPKTIVRGSKGAKDGALTLLLDEFENMSVTRSNSLRRDSPPPPARARQENGMPEEPATTARGGPGKAGSRGRFAGHSEAGGGSGDRRRAGPEKRPKSSREGSGGPQESSRDKRPLSGPDVGTPQPAGLASGAKLAAGRPFNTYPRADTDHPSRGAQGEPHDVAPNGPSAGGLAIPQSSSSSSRPPTRARGAPSPGVLGPHASEPQLAPPACTPAAPAVPGPPGPRSPQREPQRVSHEQFRAALQLVVDPGDPRSYLDNFIKIGEGSTGIVCIATVRSSGKLVAVKKMDLRKQQRRELLFNEVVIMRDYQHENVVEMYNSYLVGDELWVVMEFLEGGALTDIVTHTRMNEEQIAAVCLAVLQALSVLHAQGVIHRDIKSDSILLTHDGRVKLSDFGFCAQVSKEVPRRKSLVGTPYWMAPELISRLPYGPEVDIWSLGIMVIEMVDGEPPYFNEPPLKAMKMIRDNLPPRLKNLHKVSPSLKGFLDRLLVRDPAQRATAAELLKHPFLAKAGPPASIVPLMRQNRTR
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O96013 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K31 | Ubiquitination | Uniprot | |
| T33 | Phosphorylation | Uniprot | |
| S41 | Phosphorylation | Uniprot | |
| S74 | Phosphorylation | Uniprot | |
| K78 | Methylation | Uniprot | |
| S93 | Phosphorylation | Uniprot | |
| S97 | Phosphorylation | Uniprot | |
| S99 | Phosphorylation | Q15139 (PRKD1) | Uniprot |
| S104 | Phosphorylation | Uniprot | |
| R126 | Methylation | Uniprot | |
| S142 | Phosphorylation | Uniprot | |
| S148 | Phosphorylation | Uniprot | |
| S167 | Phosphorylation | Uniprot | |
| S181 | Phosphorylation | Uniprot | |
| T187 | Phosphorylation | Uniprot | |
| S195 | Phosphorylation | Uniprot | |
| T207 | Phosphorylation | Uniprot | |
| Y208 | Phosphorylation | Uniprot | |
| R254 | Methylation | Uniprot | |
| S258 | Phosphorylation | Uniprot | |
| S267 | Phosphorylation | Uniprot | |
| T277 | Phosphorylation | Uniprot | |
| S291 | Phosphorylation | Uniprot | |
| S443 | Phosphorylation | Uniprot | |
| S445 | Phosphorylation | Uniprot | |
| K455 | Acetylation | Uniprot | |
| K467 | Acetylation | Uniprot | |
| S474 | Phosphorylation | O96013 (PAK4) | Uniprot |
| T478 | Phosphorylation | Uniprot | |
| Y480 | Phosphorylation | Uniprot | |
| S488 | Phosphorylation | Uniprot | |
| K540 | Ubiquitination | Uniprot |
翻译修饰 - O96013 作为激酶
| Substrate | Site | Source |
|---|---|---|
| O96013-1 (PAK4) | S99 | Uniprot |
| O96013-1 (PAK4) | S104 | Uniprot |
| O96013-3 (PAK4) | S105 | Uniprot |
| O96013-3 (PAK4) | S138 | Uniprot |
| O96013-1 (PAK4) | S148 | Uniprot |
| O96013-1 (PAK4) | S167 | Uniprot |
| O96013-1 (PAK4) | S181 | Uniprot |
| O96013-1 (PAK4) | T207 | Uniprot |
| O96013-1 (PAK4) | S258 | Uniprot |
| O96013-1 (PAK4) | S291 | Uniprot |
| O96013-3 (PAK4) | T325 | Uniprot |
| O96013 (PAK4) | S474 | Uniprot |
| O96013-1 (PAK4) | T478 | Uniprot |
| P03372 (ESR1) | S305 | Uniprot |
| P04637 (TP53) | S215 | Uniprot |
| P18084 (ITGB5) | S759 | Uniprot |
| P18084 (ITGB5) | S762 | Uniprot |
| P35222 (CTNNB1) | S675 | Uniprot |
| P49023 (PXN) | S272 | Uniprot |
| P62826 (RAN) | S135 | Uniprot |
| Q15796 (SMAD2) | S465 | Uniprot |
| Q92934 (BAD) | S75 | Uniprot |
| Q92974-2 (ARHGEF2) | S885 | Uniprot |
| Q92974 (ARHGEF2) | S886 | Uniprot |
| Q93045 (STMN2) | S50 | Uniprot |
研究背景
Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, growth, proliferation or cell survival. Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments. Phosphorylates LIMK1, a kinase that also inhibits the activity of cofilin. Phosphorylates integrin beta5/ITGB5 and thus regulates cell motility. Phosphorylates ARHGEF2 and activates the downstream target RHOA that plays a role in the regulation of assembly of focal adhesions and actin stress fibers. Stimulates cell survival by phosphorylating the BCL2 antagonist of cell death BAD. Alternatively, inhibits apoptosis by preventing caspase-8 binding to death domain receptors in a kinase independent manner. Plays a role in cell-cycle progression by controlling levels of the cell-cycle regulatory protein CDKN1A and by phosphorylating RAN.
Autophosphorylated on serine residues when activated by CDC42/p21 (Ref.31). Phosphorylated on tyrosine residues upon stimulation of FGFR2 (By similarity).
Polyubiquitinated, leading to its proteasomal degradation.
Cytoplasm.
Note: Seems to shuttle between cytoplasmic compartments depending on the activating effector. For example, can be found on the cell periphery after activation of growth-factor or integrin-mediated signaling pathways.
Highest expression in prostate, testis and colon.
Interacts with FGFR2 and GRB2 (By similarity). Interacts tightly with GTP-bound but not GDP-bound CDC42/p21 and weakly with RAC1. Interacts with INKA1. Interacts with SH3RF2.
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
研究领域
· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)
· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)
· Environmental Information Processing > Signal transduction > ErbB signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Ras signaling pathway. (View pathway)
· Human Diseases > Cancers: Overview > MicroRNAs in cancer.
· Human Diseases > Cancers: Specific types > Renal cell carcinoma. (View pathway)
· Organismal Systems > Development > Axon guidance. (View pathway)
· Organismal Systems > Immune system > T cell receptor signaling pathway. (View pathway)
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