CPT2 Antibody - #DF7089

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产品: CPT2 抗体
货号: DF7089
描述: Rabbit polyclonal antibody to CPT2
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 74kDa; 74kD(Calculated).
蛋白号: P23786
RRID: AB_2839044

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MS Report
HPLC Report
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说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:100, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Zebrafish(83%), Bovine(100%), Horse(100%), Sheep(92%), Rabbit(92%), Dog(92%), Chicken(92%)
克隆:
Polyclonal
特异性:
CPT2 Antibody detects endogenous levels of total CPT2.
RRID:
AB_2839044
引用格式: Affinity Biosciences Cat# DF7089, RRID:AB_2839044.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Carnitine O palmitoyltransferase 2; Carnitine O palmitoyltransferase 2 mitochondrial; Carnitine O-palmitoyltransferase 2; Carnitine palmitoyltransferase 2; Carnitine palmitoyltransferase II; CPT 1; CPT 2; CPT II; CPT1; CPT2; CPT2_HUMAN; CPTASE; CPTII; IIAE4; mitochondrial;

抗原和靶标

免疫原:
Uniprot:

P23786(CPT2_HUMAN) >>Visit HPA database.

基因/基因ID:
CPT2(1376)
描述:
The protein encoded by this gene is a nuclear protein which is transported to the mitochondrial inner membrane. Together with carnitine palmitoyltransferase I, the encoded protein oxidizes long-chain fatty acids in the mitochondria. Defects in this gene are associated with mitochondrial long-chain fatty-acid (LCFA) oxidation disorders.
序列:
MVPRLLLRAWPRGPAVGPGAPSRPLSAGSGPGQYLQRSIVPTMHYQDSLPRLPIPKLEDTIRRYLSAQKPLLNDGQFRKTEQFCKSFENGIGKELHEQLVALDKQNKHTSYISGPWFDMYLSARDSVVLNFNPFMAFNPDPKSEYNDQLTRATNMTVSAIRFLKTLRAGLLEPEVFHLNPAKSDTITFKRLIRFVPSSLSWYGAYLVNAYPLDMSQYFRLFNSTRLPKPSRDELFTDDKARHLLVLRKGNFYIFDVLDQDGNIVSPSEIQAHLKYILSDSSPAPEFPLAYLTSENRDIWAELRQKLMSSGNEESLRKVDSAVFCLCLDDFPIKDLVHLSHNMLHGDGTNRWFDKSFNLIIAKDGSTAVHFEHSWGDGVAVLRFFNEVFKDSTQTPAVTPQSQPATTDSTVTVQKLNFELTDALKTGITAAKEKFDATMKTLTIDCVQFQRGGKEFLKKQKLSPDAVAQLAFQMAFLRQYGQTVATYESCSTAAFKHGRTETIRPASVYTKRCSEAFVREPSRHSAGELQQMMVECSKYHGQLTKEAAMGQGFDRHLFALRHLAAAKGIILPELYLDPAYGQINHNVLSTSTLSSPAVNLGGFAPVVSDGFGVGYAVHDNWIGCNVSSYPGRNAREFLQCVEKALEDMFDALEGKSIKS

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
100
Bovine
100
Sheep
92
Dog
92
Chicken
92
Rabbit
92
Zebrafish
83
Xenopus
67
Pig
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P23786 作为底物

Site PTM Type Enzyme
T42 Phosphorylation
Y45 Phosphorylation
R51 Methylation
T60 Phosphorylation
K69 Acetylation
K69 Ubiquitination
S126 Phosphorylation
S158 Phosphorylation
K182 Ubiquitination
S197 Phosphorylation
S198 Phosphorylation
Y202 Phosphorylation
S215 Phosphorylation
Y217 Phosphorylation
S223 Phosphorylation
T224 Phosphorylation
Y290 Phosphorylation
S490 Phosphorylation
T491 Phosphorylation
Y508 Phosphorylation
K510 Acetylation
K510 Methylation
S513 Phosphorylation
K544 Acetylation
R554 Methylation

研究背景

细胞定位:

Mitochondrion inner membrane>Peripheral membrane protein>Matrix side.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
蛋白家族:

Belongs to the carnitine/choline acetyltransferase family.

研究领域

· Metabolism > Lipid metabolism > Fatty acid degradation.

· Metabolism > Global and overview maps > Fatty acid metabolism.

· Organismal Systems > Endocrine system > PPAR signaling pathway.

文献引用

1). Dioscin ameliorates murine ulcerative colitis by regulating macrophage polarization. Pharmacological research, 2021 (PubMed: 34343656) [IF=9.3]
2). Mitochondrial dysfunction resulting from the down-regulation of bone morphogenetic protein 5 may cause microtia. Annals of Translational Medicine, 2021 (PubMed: 33842639)

Application: WB    Species: Human    Sample: 293T cells

Figure 6 Down-regulation of BMP5 attenuates mitochondrial fat oxidation and triglyceride synthesis. (A) The expression of CPT2 mRNA in 293T cells treated with BMP5-siRNA shows a larger decrease than that in the siNC group. (B) The expression of CPT2 mRNA in ADSCs treated with BMP5-siRNA decreased. (C) CPT2 protein expression in 293T cells treated with BMP5-siRNA shows a larger decrease than that in the siNC group. (D) The expression of DGAT2 mRNA in 293T cells treated with BMP5-siRNA decreased. (E) The expression of DGAT2 mRNA in ADSCs treated with BMP5-siRNA decreased. (F) DGAT2 protein expression in 293T cells treated with BMP5-siRNA decreased (G) Representative western blots of CPT2 and DGAT2 enzymes in 3 groups of 293T cells. *, P<0.05; **, P<0.01. BMP5, bone morphogenetic protein 5; ADSC, adipose-derived stem cells; NC, normal control; CPT2, carnitine O-palmitoyltransferase 2; DGAT2, diacylglycerol acyltransferase 2; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.

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