CX3CR1 Antibody - #DF7096

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产品: CX3CR1 抗体
货号: DF7096
描述: Rabbit polyclonal antibody to CX3CR1
应用: WB IF/ICC
反应: Human, Mouse, Rat
分子量: 44kDa; 40kD(Calculated).
蛋白号: P49238
RRID: AB_2839051

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
MSDS
说明书
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
克隆:
Polyclonal
特异性:
CX3CR1 Antibody detects endogenous levels of total CX3CR1.
RRID:
AB_2839051
引用格式: Affinity Biosciences Cat# DF7096, RRID:AB_2839051.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Beta chemokine receptor-like 1; C X3 C CKR 1; C-X3-C CKR-1; CCRL1; Chemokine C X3 C motif receptor 1; CMK BRL 1; CMK-BRL-1; CMK-BRL1; CMKBLR1; CMKDR1; CX3C chemokine receptor 1; CX3C CKR1; CX3C1_HUMAN; CX3CR1; Fractalkine receptor; G-protein coupled receptor 13; GPR13; GPRV28; V28;

抗原和靶标

免疫原:
Uniprot:

P49238(CX3C1_HUMAN) >>Visit HPA database.

基因/基因ID:
CX3CR1(1524)
表达:
P49238 CX3C1_HUMAN:

Expressed in lymphoid and neural tissues.

描述:
Fractalkine is a transmembrane protein and chemokine involved in the adhesion and migration of leukocytes. The protein encoded by this gene is a receptor for fractalkine. The encoded protein also is a coreceptor for HIV-1, and some variations in this gene lead to increased susceptibility to HIV-1 infection and rapid progression to AIDS. Four transcript variants encoding two different isoforms have been found for this gene.
序列:
MDQFPESVTENFEYDDLAEACYIGDIVVFGTVFLSIFYSVIFAIGLVGNLLVVFALTNSKKPKSVTDIYLLNLALSDLLFVATLPFWTHYLINEKGLHNAMCKFTTAFFFIGFFGSIFFITVISIDRYLAIVLAANSMNNRTVQHGVTISLGVWAAAILVAAPQFMFTKQKENECLGDYPEVLQEIWPVLRNVETNFLGFLLPLLIMSYCYFRIIQTLFSCKNHKKAKAIKLILLVVIVFFLFWTPYNVMIFLETLKLYDFFPSCDMRKDLRLALSVTETVAFSHCCLNPLIYAFAGEKFRRYLYHLYGKCLAVLCGRSVHVDFSSSESQRSRHGSVLSSNFTYHTSDGDALLLL

翻译修饰 - P49238 作为底物

Site PTM Type Enzyme
Y128 Phosphorylation
S137 Phosphorylation
Y303 Phosphorylation
S329 Phosphorylation

研究背景

功能:

Receptor for the CX3C chemokine fractalkine (CX3CL1); binds to CX3CL1 and mediates both its adhesive and migratory functions. Acts as coreceptor with CD4 for HIV-1 virus envelope protein (in vitro). Isoform 2 and isoform 3 seem to be more potent HIV-1 coreceptors than isoform 1.

翻译修饰:

This protein is not N-glycosylated which is unusual for G-protein-coupled receptors.

细胞定位:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in lymphoid and neural tissues.

亚基结构:

Found in a ternary complex with CX3CL1 and ITGAV:ITGB3 or ITGA4:ITGB1.

(Microbial infection) Interacts with human respiratory syncytial virus (HRSV) protein G; this interaction modulates host immune response.

(Microbial infection) Interacts with HIV-1 envelope polyprotein gp160.

蛋白家族:

Belongs to the G-protein coupled receptor 1 family.

研究领域

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

文献引用

1). Targeting hepatocyte MagL regulates the progression of colorectal liver metastases during liver ischemia-reperfusion. Research Square, 2022

Application: IF/ICC    Species: Mouse    Sample:

Figure 4. Hepatocyte MagL promotes inflammation resolution after LIR by affecting macrophage M2 polarization The mice in the siMagL group and the siCtrl group were subjected to partial warm ischemia for 90 minutes, and the ischemic lobes of the mice were harvested after 3 days of reperfusion. A NPCs were sorted, and the expressions of Ly6C, CD11b, CX3CR1 and their corresponding quantification were analyzed by flow cytometry. B Representative immunofluorescence images of CD206 (red) and Mertk (green) in ischemic lobes of the liver, Scale bar, 100mm. C Representative immunofluorescence images of Arg1 (red), CX3CR1 (green) and TGF-β (pink) in the ischemic lobe of the liver, Scale bar, 100mm. D Detection of M2 polarization-related markers and cytokine gene expression in liver ischemic lobes by qPCR. E The protein levels of M2 polarization-related markers in ischemic lobes of liver were detected by Western Blot. Data are presented as mean ± SD. ( *, P < 0.05; * *, P < 0.01). The data represent three separate experiments.

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