产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF7141, RRID:AB_2839095.
展开/折叠
39 kDa receptor-associated protein; A2MRAP; A2RAP; Alpha 2 macroglobulin receptor associated protein; Alpha 2 MRAP; Alpha-2-macroglobulin receptor-associated protein; Alpha-2-MRAP; AMRP_HUMAN; HBP44; Lipoprotein receptor associated protein; Low density lipoprotein receptor related protein associated protein 1; Low density lipoprotein receptor-related protein-associated protein 1; Low density lipoprotein related protein associated protein 1 alpha 2 macroglobulin receptor associated protein; Low density lipoprotein related protein associated protein 1; low density lipoprotein-related protein-associated protein 1 (alpha-2-macroglobulin receptor-associated protein 1); Lrpap1; MGC138272; MRAP; MYP23; RAP;
抗原和靶标
- P30533 AMRP_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAPRRVRSFLRGLPALLLLLLFLGPWPAASHGGKYSREKNQPKPSPKRESGEEFRMEKLNQLWEKAQRLHLPPVRLAELHADLKIQERDELAWKKLKLDGLDEDGEKEARLIRNLNVILAKYGLDGKKDARQVTSNSLSGTQEDGLDDPRLEKLWHKAKTSGKFSGEELDKLWREFLHHKEKVHEYNVLLETLSRTEEIHENVISPSDLSDIKGSVLHSRHTELKEKLRSINQGLDRLRRVSHQGYSTEAEFEEPRVIDLWDLAQSANLTDKELEAFREELKHFEAKIEKHNHYQKQLEIAHEKLRHAESVGDGERVSRSREKHALLEGRTKELGYTVKKHLQDLSGRISRARHNEL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P30533 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S45 | Phosphorylation | Uniprot | |
| S50 | Phosphorylation | Uniprot | |
| T134 | O-Glycosylation | Uniprot | |
| S135 | Phosphorylation | Uniprot | |
| S137 | Phosphorylation | Uniprot | |
| T141 | Phosphorylation | Uniprot | |
| K153 | Ubiquitination | Uniprot | |
| S165 | Phosphorylation | Uniprot | |
| Y186 | Phosphorylation | Uniprot | |
| S230 | Phosphorylation | Uniprot | |
| S242 | Phosphorylation | Uniprot | |
| S247 | Phosphorylation | Uniprot | |
| T248 | Phosphorylation | Uniprot | |
| N268 | N-Glycosylation | Uniprot | |
| K287 | Acetylation | Uniprot | |
| K304 | Ubiquitination | Uniprot | |
| S310 | Phosphorylation | Uniprot | |
| K332 | Ubiquitination | Uniprot | |
| Y336 | Phosphorylation | Uniprot | |
| T337 | O-Glycosylation | Uniprot | |
| S346 | O-Glycosylation | Uniprot | |
| R348 | Methylation | Uniprot |
研究背景
Molecular chaperone for LDL receptor-related proteins that may regulate their ligand binding activity along the secretory pathway.
N-glycosylated.
Rough endoplasmic reticulum lumen. Endoplasmic reticulum-Golgi intermediate compartment lumen. Golgi apparatus>cis-Golgi network. Golgi apparatus lumen. Endosome lumen. Cell surface.
Note: May be associated with receptors at the cell surface.
Interacts with the LRP1/alpha-2-macroglobulin receptor heavy and light chains; the interaction is transient and coincides with a reduction of ligand binding by the receptor. Interacts with LRP2/glycoprotein 330. Interacts with LRP1B; binding is followed by internalization and degradation. Interacts with LDLR. Interacts with SORL1. Interacts with LRP1; this interaction is followed by rapid internalization.
Belongs to the alpha-2-MRAP family.
研究领域
· Organismal Systems > Digestive system > Cholesterol metabolism.
限制条款
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