ABCC1 Antibody | Affinity Biosciences LTD|亲科生物官网

WB
IHC
Cites

1/4

Western blot analysis of extracts from Rat liver, using ABCC1 Antibody.

DF7148 at 1/100 staining Rat testis tissue by IHC-P.

FIGURE 3 miR-506 down-regulated MDR1/P-gp expression in HCT116-OxR.

FIGURE 5 The expressions of drug‐resistant‐related proteins (ABCC1) in hippocampus of DRE rats at 6, 7, and 9 weeks after SE.

产品:	ABCC1 抗体
货号:	DF7148
描述:	Rabbit polyclonal antibody to ABCC1
应用:	WB IHC
反应:	Human, Mouse, Rat
预测:	Zebrafish
分子量:	173kDa; 172kD(Calculated).
蛋白号:	P33527
RRID:	AB_2839101

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阻断肽(封闭肽)是特异性结合目标抗体和阻断抗体结合的多肽。这些多肽包含抗体识别的抗原表位。与阻断肽结合的抗体不再与靶蛋白上的表位结合。例如，在免疫印迹(WB)和免疫组化(IHC)中，通过比较被阻断抗体和未阻断抗体的染色，可以看到哪种染色是特异性的。

规格	价格	库存
50ul	RMB¥ 1250	现货
100ul	RMB¥ 2300	现货
200ul	RMB¥ 3000	现货

货期: 当天发货

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实验方案

产品描述

来源:

Rabbit

应用:

WB 1:500-1:1000, IHC 1:50-1:100
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:

Human,Mouse,Rat

预测:

Zebrafish(92%)

克隆:

Polyclonal

特异性:

ABCC1 Antibody detects endogenous levels of total ABCC1.

RRID:

AB_2839101
引用格式: Affinity Biosciences Cat# DF7148, RRID:AB_2839101.

偶联:

Unconjugated.

纯化:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

别名:

展开/折叠

ABC 29; ABC29; ABCC 1; ABCC; Abcc1; ATP binding cassette sub family C (CFTR/MRP) member 1; ATP binding cassette sub-family C member 1; ATP binding cassette subfamily C member 1; ATP binding cassette transporter variant ABCC1delta ex13; ATP binding cassette transporter variant ABCC1delta ex13&14; ATP binding cassette transporter variant ABCC1delta ex25; ATP binding cassette transporter variant ABCC1delta ex25&26; ATP binding cassette, sub-family C (CFTR/MRP), member 1; ATP-binding cassette sub-family C member 1; DKFZp686N04233; DKFZp781G125; GS X; GSX; Leukotriene C(4) transporter; LTC4 transporter; MRP 1; MRP; MRP1; MRP1_HUMAN; Multidrug resistance associated protein 1; Multidrug resistance protein; Multidrug resistance-associated protein 1; Multiple drug resistance associated protein; Multiple drug resistance protein 1;

抗原和靶标

免疫原:

Uniprot:

P33527(MRP1_HUMAN) >>Visit HPA database.

基因/基因ID:

ABCC1(4363)

表达:

P33527 MRP1_HUMAN:

Lung, testis and peripheral blood mononuclear cells.

描述:

The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra-and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This full transporter is a member of the MRP subfamily which is involved in multi-drug resistance. This protein functions as a multispecific organic anion transporter, with oxidized glutatione, cysteinyl leukotrienes, and activated aflatoxin B1 as substrates. This protein also transports glucuronides and sulfate conjugates of steroid hormones and bile salts. Alternatively spliced variants of this gene have been described but their full-length nature is unknown.

序列:

P33527 MRP1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MALRGFCSADGSDPLWDWNVTWNTSNPDFTKCFQNTVLVWVPCFYLWACFPFYFLYLSRHDRGYIQMTPLNKTKTALGFLLWIVCWADLFYSFWERSRGIFLAPVFLVSPTLLGITMLLATFLIQLERRKGVQSSGIMLTFWLVALVCALAILRSKIMTALKEDAQVDLFRDITFYVYFSLLLIQLVLSCFSDRSPLFSETIHDPNPCPESSASFLSRITFWWITGLIVRGYRQPLEGSDLWSLNKEDTSEQVVPVLVKNWKKECAKTRKQPVKVVYSSKDPAQPKESSKVDANEEVEALIVKSPQKEWNPSLFKVLYKTFGPYFLMSFFFKAIHDLMMFSGPQILKLLIKFVNDTKAPDWQGYFYTVLLFVTACLQTLVLHQYFHICFVSGMRIKTAVIGAVYRKALVITNSARKSSTVGEIVNLMSVDAQRFMDLATYINMIWSAPLQVILALYLLWLNLGPSVLAGVAVMVLMVPVNAVMAMKTKTYQVAHMKSKDNRIKLMNEILNGIKVLKLYAWELAFKDKVLAIRQEELKVLKKSAYLSAVGTFTWVCTPFLVALCTFAVYVTIDENNILDAQTAFVSLALFNILRFPLNILPMVISSIVQASVSLKRLRIFLSHEELEPDSIERRPVKDGGGTNSITVRNATFTWARSDPPTLNGITFSIPEGALVAVVGQVGCGKSSLLSALLAEMDKVEGHVAIKGSVAYVPQQAWIQNDSLRENILFGCQLEEPYYRSVIQACALLPDLEILPSGDRTEIGEKGVNLSGGQKQRVSLARAVYSNADIYLFDDPLSAVDAHVGKHIFENVIGPKGMLKNKTRILVTHSMSYLPQVDVIIVMSGGKISEMGSYQELLARDGAFAEFLRTYASTEQEQDAEENGVTGVSGPGKEAKQMENGMLVTDSAGKQLQRQLSSSSSYSGDISRHHNSTAELQKAEAKKEETWKLMEADKAQTGQVKLSVYWDYMKAIGLFISFLSIFLFMCNHVSALASNYWLSLWTDDPIVNGTQEHTKVRLSVYGALGISQGIAVFGYSMAVSIGGILASRCLHVDLLHSILRSPMSFFERTPSGNLVNRFSKELDTVDSMIPEVIKMFMGSLFNVIGACIVILLATPIAAIIIPPLGLIYFFVQRFYVASSRQLKRLESVSRSPVYSHFNETLLGVSVIRAFEEQERFIHQSDLKVDENQKAYYPSIVANRWLAVRLECVGNCIVLFAALFAVISRHSLSAGLVGLSVSYSLQVTTYLNWLVRMSSEMETNIVAVERLKEYSETEKEAPWQIQETAPPSSWPQVGRVEFRNYCLRYREDLDFVLRHINVTINGGEKVGIVGRTGAGKSSLTLGLFRINESAEGEIIIDGINIAKIGLHDLRFKITIIPQDPVLFSGSLRMNLDPFSQYSDEEVWTSLELAHLKDFVSALPDKLDHECAEGGENLSVGQRQLVCLARALLRKTKILVLDEATAAVDLETDDLIQSTIRTQFEDCTVLTIAHRLNTIMDYTRVIVLDKGEIQEYGAPSDLLQQRGLFYSMAKDAGLV

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species

Results

Score

Zebrafish

Bovine

Dog

Chicken

Xenopus

Pig

Horse

Sheep

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P33527 作为底物

P33527

Site	PTM Type	Enzyme	Source
N19	N-Glycosylation		Uniprot
N23	N-Glycosylation		Uniprot
K72	Ubiquitination		Uniprot
K246	Ubiquitination		Uniprot
T249	Phosphorylation	P68400 (CSNK2A1)	Uniprot
K259	Ubiquitination		Uniprot
K274	Ubiquitination		Uniprot
Y277	Phosphorylation		Uniprot
S279	Phosphorylation		Uniprot
K280	Ubiquitination		Uniprot
S289	Phosphorylation		Uniprot
K290	Ubiquitination		Uniprot
K303	Ubiquitination		Uniprot
K307	Ubiquitination		Uniprot
K315	Ubiquitination		Uniprot
K396	Ubiquitination		Uniprot
Y404	Phosphorylation		Uniprot
K406	Ubiquitination		Uniprot
K416	Ubiquitination		Uniprot
K488	Ubiquitination		Uniprot
K496	Ubiquitination		Uniprot
S497	Phosphorylation		Uniprot
K498	Acetylation		Uniprot
K498	Ubiquitination		Uniprot
K503	Acetylation		Uniprot
K503	Ubiquitination		Uniprot
K513	Ubiquitination		Uniprot
K525	Ubiquitination		Uniprot
K527	Ubiquitination		Uniprot
K636	Ubiquitination		Uniprot
K764	Ubiquitination		Uniprot
K773	Ubiquitination		Uniprot
K804	Ubiquitination		Uniprot
K814	Ubiquitination		Uniprot
K818	Ubiquitination		Uniprot
S847	Phosphorylation		Uniprot
Y852	Phosphorylation		Uniprot
S871	Phosphorylation		Uniprot
T872	Phosphorylation		Uniprot
K891	Ubiquitination		Uniprot
K894	Ubiquitination		Uniprot
S905	Phosphorylation		Uniprot
K908	Ubiquitination		Uniprot
S915	Phosphorylation		Uniprot
S916	Phosphorylation		Uniprot
S917	Phosphorylation		Uniprot
S918	Phosphorylation		Uniprot
S919	Phosphorylation		Uniprot
Y920	Phosphorylation		Uniprot
S921	Phosphorylation		Uniprot
S925	Phosphorylation		Uniprot
S930	Phosphorylation		Uniprot
T931	Phosphorylation		Uniprot
K936	Ubiquitination		Uniprot
K946	Ubiquitination		Uniprot
K952	Ubiquitination		Uniprot
S961	Phosphorylation		Uniprot
N1006	N-Glycosylation		Uniprot
K1078	Ubiquitination		Uniprot
S1145	Phosphorylation		Uniprot
K1181	Ubiquitination		Uniprot
K1187	Ubiquitination		Uniprot
Y1243	Phosphorylation		Uniprot
K1265	Ubiquitination		Uniprot
K1272	Ubiquitination		Uniprot
K1322	Ubiquitination		Uniprot
K1333	Ubiquitination		Uniprot
K1360	Ubiquitination		Uniprot
K1369	Ubiquitination		Uniprot
K1502	Ubiquitination		Uniprot
Y1508	Phosphorylation		Uniprot
Y1522	Phosphorylation		Uniprot
S1523	Phosphorylation		Uniprot
K1526	Ubiquitination		Uniprot

研究背景

P33527_MRP1_HUMAN

功能:

Mediates export of organic anions and drugs from the cytoplasm. Mediates ATP-dependent transport of glutathione and glutathione conjugates, leukotriene C4, estradiol-17-beta-o-glucuronide, methotrexate, antiviral drugs and other xenobiotics. Confers resistance to anticancer drugs by decreasing accumulation of drug in cells, and by mediating ATP- and GSH-dependent drug export. Hydrolyzes ATP with low efficiency. Catalyzes the export of sphingosine 1-phosphate from mast cells independently of their degranulation. Participates in inflammatory response by allowing export of leukotriene C4 from leukotriene C4-synthezing cells (By similarity).

细胞定位:

Cell membrane>Multi-pass membrane protein.

组织特异性:

Lung, testis and peripheral blood mononuclear cells.

蛋白家族:

Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.

研究领域

· Environmental Information Processing > Membrane transport > ABC transporters.

· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway. (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > Antifolate resistance.

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Organismal Systems > Digestive system > Vitamin digestion and absorption.

文献引用

1). 4′-OH as the Action Site of Lipids and MRP1 for Enhanced Transdermal Delivery of Flavonoids. ACS Applied Materials & Interfaces, 2023 (PubMed: 36913526) [IF=9.5]

2). miR-506 enhances the sensitivity of human colorectal cancer cells to oxaliplatin by suppressing MDR1/P-gp expression. CELL PROLIFERATION, 2017 (PubMed: 28217977) [IF=8.5]

Application: WB Species: human Sample:

FIGURE 3 miR-506 down-regulated MDR1/P-gp expression in HCT116-OxR. A, The mRNA level of MDR1 was decreased after transfection with the miR-506 mimic of the relative chemoresistant genes as demonstrated by qRT-PCR. B, The protein level of MDR1 was decreased after transfection with the miR-506 mimic of the relative chemoresistant proteins as demonstrated by Western blot. C, Expression of P-gp detected by immunofluorescence staining.HCT116-OxR-miR-506 cells showed low levels of fluorescent staining of P-gp, whereas maximal staining of P-gp was observed in HCT116-OxR cells, readily distinguished from background. Zoom: 200×. *P<.05)

3). miR‐485's anti‐drug resistant epilepsy effects by regulating SV2A/PSD‐95 and targeting ABCC1 and neuronal signaling‐transduction proteins in hippocampus of rats. Brain and Behavior, 2021 (PubMed: 34291586) [IF=3.1]

Application: WB Species: Rat Sample: hippocampus

FIGURE 5 The expressions of drug‐resistant‐related proteins (ABCC1) in hippocampus of DRE rats at 6, 7, and 9 weeks after SE. *p < .05 versus the control group; # p < .5 versus the epilepsy group

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ABCC1 Antibody - #DF7148

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