ABCC5 Antibody - #DF7149

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产品: ABCC5 抗体
货号: DF7149
描述: Rabbit polyclonal antibody to ABCC5
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Chicken
分子量: 161kDa; 161kD(Calculated).
蛋白号: O15440
RRID: AB_2839102

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MS Report
HPLC Report
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Chicken(100%)
克隆:
Polyclonal
特异性:
ABCC5 Antibody detects endogenous levels of total ABCC5.
RRID:
AB_2839102
引用格式: Affinity Biosciences Cat# DF7149, RRID:AB_2839102.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

ABC 33; ABC33; ABCC 5; Abcc5; ATP binding cassette sub family C (CFTR/MRP) member 5; ATP binding cassette sub family C member 5; ATP-binding cassette sub-family C member 5; Canalicular multispecific organic anion transporter C; DKFZp686C1782; EST277145; MOAT C; MOAT-C; MOATC; MRP 5; MRP5_HUMAN; Multi specific organic anion tranporter C; Multi-specific organic anion transporter C; Multidrug resistance associated protein 5; Multidrug resistance-associated protein 5; pABC 11; pABC11; SMRP;

抗原和靶标

免疫原:
Uniprot:

O15440(MRP5_HUMAN) >>Visit HPA database.

基因/基因ID:
ABCC5(10057)
表达:
O15440 MRP5_HUMAN:

All isoforms are equally expressed in retina.

描述:
The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. This protein functions in the cellular export of its substrate, cyclic nucleotides. This export contributes to the degradation of phosphodiesterases and possibly an elimination pathway for cyclic nucleotides. Studies show that this protein provides resistance to thiopurine anticancer drugs, 6-mercatopurine and thioguanine, and the anti-HIV drug 9-(2-phosphonylmethoxyethyl)adenine.
序列:
MKDIDIGKEYIIPSPGYRSVRERTSTSGTHRDREDSKFRRTRPLECQDALETAARAEGLSLDASMHSQLRILDEEHPKGKYHHGLSALKPIRTTSKHQHPVDNAGLFSCMTFSWLSSLARVAHKKGELSMEDVWSLSKHESSDVNCRRLERLWQEELNEVGPDAASLRRVVWIFCRTRLILSIVCLMITQLAGFSGPAFMVKHLLEYTQATESNLQYSLLLVLGLLLTEIVRSWSLALTWALNYRTGVRLRGAILTMAFKKILKLKNIKEKSLGELINICSNDGQRMFEAAAVGSLLAGGPVVAILGMIYNVIILGPTGFLGSAVFILFYPAMMFASRLTAYFRRKCVAATDERVQKMNEVLTYIKFIKMYAWVKAFSQSVQKIREEERRILEKAGYFQSITVGVAPIVVVIASVVTFSVHMTLGFDLTAAQAFTVVTVFNSMTFALKVTPFSVKSLSEASVAVDRFKSLFLMEEVHMIKNKPASPHIKIEMKNATLAWDSSHSSIQNSPKLTPKMKKDKRASRGKKEKVRQLQRTEHQAVLAEQKGHLLLDSDERPSPEEEEGKHIHLGHLRLQRTLHSIDLEIQEGKLVGICGSVGSGKTSLISAILGQMTLLEGSIAISGTFAYVAQQAWILNATLRDNILFGKEYDEERYNSVLNSCCLRPDLAILPSSDLTEIGERGANLSGGQRQRISLARALYSDRSIYILDDPLSALDAHVGNHIFNSAIRKHLKSKTVLFVTHQLQYLVDCDEVIFMKEGCITERGTHEELMNLNGDYATIFNNLLLGETPPVEINSKKETSGSQKKSQDKGPKTGSVKKEKAVKPEEGQLVQLEEKGQGSVPWSVYGVYIQAAGGPLAFLVIMALFMLNVGSTAFSTWWLSYWIKQGSGNTTVTRGNETSVSDSMKDNPHMQYYASIYALSMAVMLILKAIRGVVFVKGTLRASSRLHDELFRRILRSPMKFFDTTPTGRILNRFSKDMDEVDVRLPFQAEMFIQNVILVFFCVGMIAGVFPWFLVAVGPLVILFSVLHIVSRVLIRELKRLDNITQSPFLSHITSSIQGLATIHAYNKGQEFLHRYQELLDDNQAPFFLFTCAMRWLAVRLDLISIALITTTGLMIVLMHGQIPPAYAGLAISYAVQLTGLFQFTVRLASETEARFTSVERINHYIKTLSLEAPARIKNKAPSPDWPQEGEVTFENAEMRYRENLPLVLKKVSFTIKPKEKIGIVGRTGSGKSSLGMALFRLVELSGGCIKIDGVRISDIGLADLRSKLSIIPQEPVLFSGTVRSNLDPFNQYTEDQIWDALERTHMKECIAQLPLKLESEVMENGDNFSVGERQLLCIARALLRHCKILILDEATAAMDTETDLLIQETIREAFADCTMLTIAHRLHTVLGSDRIMVLAQGQVVEFDTPSVLLSNDSSRFYAMFAAAENKVAVKG

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Chicken
100
Rabbit
100
Xenopus
56
Dog
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - O15440 作为底物

Site PTM Type Enzyme
K8 Ubiquitination
Y10 Phosphorylation
S14 Phosphorylation
Y17 Phosphorylation
S19 Phosphorylation
S60 Phosphorylation
S64 Phosphorylation
S67 Phosphorylation
K78 Ubiquitination
K89 Ubiquitination
T351 Phosphorylation
K383 Ubiquitination
S485 Phosphorylation
K493 Ubiquitination
S501 Phosphorylation
S505 Phosphorylation
S509 Phosphorylation
T513 Phosphorylation
K546 Ubiquitination
S553 Phosphorylation
S558 Phosphorylation
K565 Ubiquitination
S686 Phosphorylation
K798 Acetylation
T800 Phosphorylation
K805 Acetylation
K806 Acetylation
S807 Phosphorylation
T814 Phosphorylation
K821 Ubiquitination
K824 Ubiquitination
K977 Ubiquitination
Y1166 Phosphorylation
Y1202 Phosphorylation
K1211 Ubiquitination
K1212 Ubiquitination
T1216 Phosphorylation
K1218 Ubiquitination
K1222 Ubiquitination
S1268 Phosphorylation
K1309 Ubiquitination
Y1423 Phosphorylation

研究背景

功能:

Acts as a multispecific organic anion pump which can transport nucleotide analogs.

细胞定位:

Membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

All isoforms are equally expressed in retina.

蛋白家族:

Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.

研究领域

· Environmental Information Processing > Membrane transport > ABC transporters.

· Human Diseases > Drug resistance: Antineoplastic > Antifolate resistance.

文献引用

1). Brusatol Enhances the Chemotherapy Efficacy of Gemcitabine in Pancreatic Cancer via the Nrf2 Signalling Pathway. Oxidative Medicine and Cellular Longevity, 2018 (PubMed: 29849873)

Application: WB    Species: human    Sample: pancreatic cancer cells

Figure 2:| Brusatol inhibited the Nrf2 pathway and increase ROS accumulation in pancreatic cancer cells. (a) 0.5μM brusatol was utilized to treat the expression of Nrf2 in PATU-8988 cells for the indicated time points. (b) 0.5μM brusatol lowered the protein levels of Nrf2 and its downstreamgenesafter8-hourtreatments.

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