HCRTR2 Antibody - #DF7162

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产品: HCRTR2 抗体
货号: DF7162
描述: Rabbit polyclonal antibody to HCRTR2
应用: WB IHC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 51kDa; 51kD(Calculated).
蛋白号: O43614
RRID: AB_2839114

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
克隆:
Polyclonal
特异性:
HCRTR2 Antibody detects endogenous levels of total HCRTR2.
RRID:
AB_2839114
引用格式: Affinity Biosciences Cat# DF7162, RRID:AB_2839114.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Hcr tr2; Hcrt receptor 2; Hcrtr2; Hypocretin (orexin) receptor 2; Hypocretin receptor 2; Hypocretin receptor type 2; Orexin 2 receptor; Orexin A/Orexin B receptor; Orexin receptor 2; Orexin receptor type 2; Orexin type 2 receptor; Ox-2-R; Ox2-R; Ox2r; OX2R_HUMAN; Type 2 hypocretin receptor; Type 2 orexin receptor;

抗原和靶标

免疫原:
Uniprot:

O43614(OX2R_HUMAN) >>Visit HPA database.

基因/基因ID:
HCRTR2(3062)
描述:
The protein encoded by this gene is a G-protein coupled receptor involved in the regulation of feeding behavior. The encoded protein binds the hypothalamic neuropeptides orexin A and orexin B. A related gene (HCRTR1) encodes a G-protein coupled receptor that selectively binds orexin A.
序列:
MSGTKLEDSPPCRNWSSASELNETQEPFLNPTDYDDEEFLRYLWREYLHPKEYEWVLIAGYIIVFVVALIGNVLVCVAVWKNHHMRTVTNYFIVNLSLADVLVTITCLPATLVVDITETWFFGQSLCKVIPYLQTVSVSVSVLTLSCIALDRWYAICHPLMFKSTAKRARNSIVIIWIVSCIIMIPQAIVMECSTVFPGLANKTTLFTVCDERWGGEIYPKMYHICFFLVTYMAPLCLMVLAYLQIFRKLWCRQIPGTSSVVQRKWKPLQPVSQPRGPGQPTKSRMSAVAAEIKQIRARRKTARMLMIVLLVFAICYLPISILNVLKRVFGMFAHTEDRETVYAWFTFSHWLVYANSAANPIIYNFLSGKFREEFKAAFSCCCLGVHHRQEDRLTRGRTSTESRKSLTTQISNFDNISKLSEQVVLTSISTLPAANGAGPLQNW

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - O43614 作为底物

Site PTM Type Enzyme
Y223 Phosphorylation
T231 Phosphorylation
Y243 Phosphorylation
S273 Phosphorylation
Y317 Phosphorylation
S321 Phosphorylation

研究背景

功能:

Nonselective, high-affinity receptor for both orexin-A and orexin-B neuropeptides. Triggers an increase in cytoplasmic Ca(2+) levels in response to orexin-A binding.

细胞定位:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
蛋白家族:

The N-terminal region is required for orexin signaling.

Belongs to the G-protein coupled receptor 1 family.

研究领域

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

文献引用

1). Soporific effect of modified Suanzaoren Decoction on mice models of insomnia by regulating Orexin-A and HPA axis homeostasis. Biomedicine & Pharmacotherapy, 2021 (PubMed: 34509822) [IF=7.5]

Application: WB    Species: Mice    Sample: hypothalamus tissue

Fig. 9. Effect of MSZRD on the level of Orexin-A and the expression of OX2R. (a) The level of Orexin-A in serum was measured by ELISA kits. (b) OX2R protein was quantified as IOD/Area by Imagine Pro. (c) The expression of OX2R in hypothalamus was detected by western blot and (d) Statistical analysis on OX2R. (e) The expression of OX2R protein in hypothalamus tissue was examined by immunohistochemical staining (400×，brown-yellow granules indicate positive expression). Data are expressed as the mean ± SD. # P < 0.05, ## P < 0.01 compare with control group; *P < 0.05 and **P < 0.01 compare with model group.

Application: IHC    Species: Mice    Sample: hypothalamus tissue

Fig. 9. Effect of MSZRD on the level of Orexin-A and the expression of OX2R. (a) The level of Orexin-A in serum was measured by ELISA kits. (b) OX2R protein was quantified as IOD/Area by Imagine Pro. (c) The expression of OX2R in hypothalamus was detected by western blot and (d) Statistical analysis on OX2R. (e) The expression of OX2R protein in hypothalamus tissue was examined by immunohistochemical staining (400×，brown-yellow granules indicate positive expression). Data are expressed as the mean ± SD. # P < 0.05, ## P < 0.01 compare with control group; *P < 0.05 and **P < 0.01 compare with model group.

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