产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF7165, RRID:AB_2839117.
展开/折叠
2310001G03Rik; PAR 4; PAR-4; Pawr; PAWR_HUMAN; PRKC Apoptosis WT1 Regulator; PRKC apoptosis WT1 regulator protein; Prostate apoptosis response 4 protein; Prostate apoptosis response protein 4; prostate apoptosis response protein PAR-4; Transcriptional repressor Par-4-like protein PAWR; Transcriptional repressor PAR4; WT1 Interacting Protein;
抗原和靶标
Widely expressed. Expression is elevated in various neurodegenerative diseases such as amyotrophic lateral sclerosis, Alzheimer, Parkinson and Huntington diseases and stroke. Down-regulated in several cancers.
- Q96IZ0 PAWR_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MATGGYRTSSGLGGSTTDFLEEWKAKREKMRAKQNPPGPAPPGGGSSDAAGKPPAGALGTPAAAAANELNNNLPGGAPAAPAVPGPGGVNCAVGSAMLTRAAPGPRRSEDEPPAASASAAPPPQRDEEEPDGVPEKGKSSGPSARKGKGQIEKRKLREKRRSTGVVNIPAAECLDEYEDDEAGQKERKREDAITQQNTIQNEAVNLLDPGSSYLLQEPPRTVSGRYKSTTSVSEEDVSSRYSRTDRSGFPRYNRDANVSGTLVSSSTLEKKIEDLEKEVVRERQENLRLVRLMQDKEEMIGKLKEEIDLLNRDLDDIEDENEQLKQENKTLLKVVGQLTR
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q96IZ0 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T8 | Phosphorylation | Uniprot | |
| S15 | Phosphorylation | Uniprot | |
| S95 | Phosphorylation | Uniprot | |
| T99 | Phosphorylation | Uniprot | |
| S108 | Phosphorylation | Uniprot | |
| K146 | Methylation | Uniprot | |
| S162 | Phosphorylation | Uniprot | |
| T163 | Phosphorylation | Uniprot | |
| C173 | S-Nitrosylation | Uniprot | |
| Y177 | Phosphorylation | Uniprot | |
| S223 | Phosphorylation | Uniprot | |
| Y226 | Phosphorylation | Uniprot | |
| K227 | Methylation | Uniprot | |
| K227 | Ubiquitination | Uniprot | |
| S228 | Phosphorylation | Uniprot | |
| T229 | Phosphorylation | Uniprot | |
| T230 | Phosphorylation | Uniprot | |
| S231 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S233 | Phosphorylation | Uniprot | |
| Y241 | Phosphorylation | Uniprot | |
| S242 | Phosphorylation | Uniprot | |
| S259 | Phosphorylation | Uniprot | |
| T261 | Phosphorylation | Uniprot | |
| T267 | Phosphorylation | Uniprot | |
| K277 | Ubiquitination | Uniprot | |
| K296 | Acetylation | Uniprot | |
| K296 | Ubiquitination | Uniprot | |
| K302 | Acetylation | Uniprot | |
| K304 | Ubiquitination | Uniprot | |
| K329 | Ubiquitination | Uniprot | |
| K333 | Ubiquitination | Uniprot |
研究背景
Pro-apoptotic protein capable of selectively inducing apoptosis in cancer cells, sensitizing the cells to diverse apoptotic stimuli and causing regression of tumors in animal models. Induces apoptosis in certain cancer cells by activation of the Fas prodeath pathway and coparallel inhibition of NF-kappa-B transcriptional activity. Inhibits the transcriptional activation and augments the transcriptional repression mediated by WT1. Down-regulates the anti-apoptotic protein BCL2 via its interaction with WT1. Seems also to be a transcriptional repressor by itself. May be directly involved in regulating the amyloid precursor protein (APP) cleavage activity of BACE1.
Preferentially phosphorylated at the Thr-163 by PKC in cancer cells.
Cytoplasm. Nucleus.
Note: Mainly cytoplasmic in absence of apoptosis signal and in normal cells. Nuclear in most cancer cell lines. Nuclear entry seems to be essential but not sufficient for apoptosis (By similarity). Nuclear localization includes nucleoplasm and PML nuclear bodies.
Widely expressed. Expression is elevated in various neurodegenerative diseases such as amyotrophic lateral sclerosis, Alzheimer, Parkinson and Huntington diseases and stroke. Down-regulated in several cancers.
Homooligomer. Interacts (via the C-terminal region) with WT1. Interacts with THAP1. Interacts with AATF. Interacts with BACE1. Interacts with SPSB1 (via B30.2/SPRY domain); this interaction is direct and occurs in association with the Elongin BC complex. Interacts with SPSB2 (via B30.2/SPRY domain); this interaction occurs in association with the Elongin BC complex. Interacts with SPSB4 (via B30.2/SPRY domain); this interaction occurs in association with the Elongin BC complex. Component of a ternary complex composed of SQSTM1 and PRKCZ. Interacts with actin (By similarity).
The leucine-zipper domain is not essential for apoptosis, but is required for sensitization of cells to exogenous apoptotic insults and for interaction with its partners.
The SAC domain is a death-inducing domain selective for apoptosis induction in cancer cells. This domain is essential for nuclear entry, Fas activation, inhibition of NF-kappa-B activity and induction of apoptosis in cancer cells (By similarity).
The B30.2/SPRY domain-binding motif mediates recognition by proteins containing a B30.2/SPRY domain.
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