(1)
(5)
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF7166, RRID:AB_2839118.
展开/折叠
0710008C04Rik; 2610010A15Rik; Par 6 partitioning defective 6 C elegans homolog alpha; Par 6 partitioning defective 6 homolog alpha; Par 6 partitioning defective 6 homolog alpha C elegans; PAR 6A; PAR-6 alpha; PAR-6; par-6 family cell polarity regulator alpha; PAR-6A; PAR6; PAR6A_HUMAN; PAR6alpha; PAR6C; Pard6a; Partitioning defective 6 homolog alpha; partitioning-defective protein 6; partitioning-defective protein 6, C. elegans, homolog of, alpha; Tax interacting protein 40; Tax interaction protein 40; TAX40; TIP 40; TIP-40; TIP40;
抗原和靶标
Expressed in pancreas, skeletal muscle, brain and heart. Weakly expressed in kidney and placenta.
- Q9NPB6 PAR6A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MARPQRTPARSPDSIVEVKSKFDAEFRRFALPRASVSGFQEFSRLLRAVHQIPGLDVLLGYTDAHGDLLPLTNDDSLHRALASGPPPLRLLVQKRAEADSSGLAFASNSLQRRKKGLLLRPVAPLRTRPPLLISLPQDFRQVSSVIDVDLLPETHRRVRLHKHGSDRPLGFYIRDGMSVRVAPQGLERVPGIFISRLVRGGLAESTGLLAVSDEILEVNGIEVAGKTLDQVTDMMVANSHNLIVTVKPANQRNNVVRGASGRLTGPPSAGPGPAEPDSDDDSSDLVIENRQPPSSNGLSQGPPCWDLHPGCRHPGTRSSLPSLDDQEQASSGWGSRIRGDGSGFSL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9NPB6 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K19 | Ubiquitination | Uniprot | |
| S100 | Phosphorylation | Uniprot | |
| S101 | Phosphorylation | Uniprot | |
| S107 | Phosphorylation | Uniprot | |
| S109 | Phosphorylation | Uniprot | |
| S165 | Phosphorylation | Uniprot | |
| S278 | Phosphorylation | Uniprot | |
| S282 | Phosphorylation | Uniprot | |
| S345 | Phosphorylation | P37173 (TGFBR2) , P41743 (PRKCI) | Uniprot |
研究背景
Adapter protein involved in asymmetrical cell division and cell polarization processes. Probably involved in the formation of epithelial tight junctions. Association with PARD3 may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins. Regulates centrosome organization and function. Essential for the centrosomal recruitment of key proteins that control centrosomal microtubule organization.
Phosphorylated by the TGF-beta receptor.
Cytoplasm. Cell membrane. Cell projection>Ruffle. Cell junction>Tight junction. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome>Centriolar satellite. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.
Note: Colocalizes with GTP-bound CDC42 or RAC1 at membrane ruffles and with PARD3 and PRKCI at epithelial tight junctions. Recruited to the centrosome by a microtubule and dynein-dynactin-dependent mechanism.
Expressed in pancreas, skeletal muscle, brain and heart. Weakly expressed in kidney and placenta.
Interacts with MAP2K5 (By similarity). Interacts with PARD3. Interacts with GTP-bound forms of CDC42, ARHQ/TC10 and RAC1. Interacts with the N-terminal part of PRKCI and PRKCZ. Part of a complex with PARD3, CDC42 or RAC1 and PRKCI or PRKCZ. Part of a complex with LLGL1 and PRKCI (By similarity). Interacts with human T-cell leukemia virus type I TAX protein. Interacts with MPP5 and CRB3. Interacts with TGFBR1; involved in TGF-beta induced epithelial to mesenchymal transition. Interacts with ECT2 ('Thr-359' phosphorylated form) and PRKCI. Interacts with DCTN1 and PCM1.
The pseudo-CRIB domain together with the PDZ domain is required for the interaction with Rho small GTPases.
The PB1 domain mediates interactions with MAP2K5.
The PDZ domain mediates the interaction with CRB3.
Belongs to the PAR6 family.
研究领域
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)
· Environmental Information Processing > Signal transduction > Rap1 signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Hippo signaling pathway. (View pathway)
· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.
· Organismal Systems > Development > Axon guidance. (View pathway)
文献引用
Application: WB Species: Mouse Sample: calcification tissue
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