产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF7176, RRID:AB_2839128.
展开/折叠
DNA repair protein RAD50; hRAD50; hsRAD50; Mrell; NBSLD; Rad 50; RAD50 2; rad50; RAD50 homolog (S cerevisiae); RAD50 homolog; RAD50 PEN; RAD50, S. cerevisiae, homolog of; RAD50_HUMAN; RAD502; Rad50l; Truncated RAD50 protein;
抗原和靶标
Expressed at very low level in most tissues, except in testis where it is expressed at higher level. Expressed in fibroblasts.
- Q92878 RAD50_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSRIEKMSILGVRSFGIEDKDKQIITFFSPLTILVGPNGAGKTTIIECLKYICTGDFPPGTKGNTFVHDPKVAQETDVRAQIRLQFRDVNGELIAVQRSMVCTQKSKKTEFKTLEGVITRTKHGEKVSLSSKCAEIDREMISSLGVSKAVLNNVIFCHQEDSNWPLSEGKALKQKFDEIFSATRYIKALETLRQVRQTQGQKVKEYQMELKYLKQYKEKACEIRDQITSKEAQLTSSKEIVKSYENELDPLKNRLKEIEHNLSKIMKLDNEIKALDSRKKQMEKDNSELEEKMEKVFQGTDEQLNDLYHNHQRTVREKERKLVDCHRELEKLNKESRLLNQEKSELLVEQGRLQLQADRHQEHIRARDSLIQSLATQLELDGFERGPFSERQIKNFHKLVRERQEGEAKTANQLMNDFAEKETLKQKQIDEIRDKKTGLGRIIELKSEILSKKQNELKNVKYELQQLEGSSDRILELDQELIKAERELSKAEKNSNVETLKMEVISLQNEKADLDRTLRKLDQEMEQLNHHTTTRTQMEMLTKDKADKDEQIRKIKSRHSDELTSLLGYFPNKKQLEDWLHSKSKEINQTRDRLAKLNKELASSEQNKNHINNELKRKEEQLSSYEDKLFDVCGSQDFESDLDRLKEEIEKSSKQRAMLAGATAVYSQFITQLTDENQSCCPVCQRVFQTEAELQEVISDLQSKLRLAPDKLKSTESELKKKEKRRDEMLGLVPMRQSIIDLKEKEIPELRNKLQNVNRDIQRLKNDIEEQETLLGTIMPEEESAKVCLTDVTIMERFQMELKDVERKIAQQAAKLQGIDLDRTVQQVNQEKQEKQHKLDTVSSKIELNRKLIQDQQEQIQHLKSTTNELKSEKLQISTNLQRRQQLEEQTVELSTEVQSLYREIKDAKEQVSPLETTLEKFQQEKEELINKKNTSNKIAQDKLNDIKEKVKNIHGYMKDIENYIQDGKDDYKKQKETELNKVIAQLSECEKHKEKINEDMRLMRQDIDTQKIQERWLQDNLTLRKRNEELKEVEEERKQHLKEMGQMQVLQMKSEHQKLEENIDNIKRNHNLALGRQKGYEEEIIHFKKELREPQFRDAEEKYREMMIVMRTTELVNKDLDIYYKTLDQAIMKFHSMKMEEINKIIRDLWRSTYRGQDIEYIEIRSDADENVSASDKRRNYNYRVVMLKGDTALDMRGRCSAGQKVLASLIIRLALAETFCLNCGIIALDEPTTNLDRENIESLAHALVEIIKSRSQQRNFQLLVITHDEDFVELLGRSEYVEKFYRIKKNIDQCSEIVKCSVSSLGFNVH
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q92878 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K6 | Ubiquitination | Uniprot | |
| K20 | Methylation | Uniprot | |
| K62 | Ubiquitination | Uniprot | |
| K71 | Ubiquitination | Uniprot | |
| K112 | Ubiquitination | Uniprot | |
| K126 | Ubiquitination | Uniprot | |
| S128 | Phosphorylation | Uniprot | |
| S130 | Phosphorylation | Uniprot | |
| K132 | Ubiquitination | Uniprot | |
| K175 | Ubiquitination | Uniprot | |
| K187 | Ubiquitination | Uniprot | |
| R193 | Methylation | Uniprot | |
| T198 | Phosphorylation | Uniprot | |
| K211 | Ubiquitination | Uniprot | |
| K230 | Ubiquitination | Uniprot | |
| K238 | Ubiquitination | Uniprot | |
| K242 | Ubiquitination | Uniprot | |
| K252 | Ubiquitination | Uniprot | |
| K256 | Ubiquitination | Uniprot | |
| S263 | Phosphorylation | Uniprot | |
| K264 | Acetylation | Uniprot | |
| K273 | Ubiquitination | Uniprot | |
| K280 | Ubiquitination | Uniprot | |
| K295 | Ubiquitination | Uniprot | |
| Y308 | Phosphorylation | Uniprot | |
| K343 | Ubiquitination | Uniprot | |
| R359 | Methylation | Uniprot | |
| K398 | Acetylation | Uniprot | |
| K446 | Ubiquitination | Uniprot | |
| K453 | Acetylation | Uniprot | |
| K461 | Acetylation | Uniprot | |
| K461 | Ubiquitination | Uniprot | |
| S470 | Phosphorylation | Uniprot | |
| S471 | Phosphorylation | Uniprot | |
| R473 | Methylation | Uniprot | |
| K483 | Ubiquitination | Uniprot | |
| K520 | Ubiquitination | Uniprot | |
| K585 | Acetylation | Uniprot | |
| K599 | Ubiquitination | Uniprot | |
| K616 | Ubiquitination | Uniprot | |
| S623 | Phosphorylation | Uniprot | |
| S624 | Phosphorylation | Uniprot | |
| S635 | Phosphorylation | Q13315 (ATM) , Q13535 (ATR) | Uniprot |
| S640 | Phosphorylation | Uniprot | |
| T690 | Phosphorylation | Uniprot | |
| K743 | Ubiquitination | Uniprot | |
| K753 | Ubiquitination | Uniprot | |
| R797 | Methylation | Uniprot | |
| R823 | Methylation | Uniprot | |
| K838 | Ubiquitination | Uniprot | |
| K845 | Ubiquitination | Uniprot | |
| K851 | Ubiquitination | Uniprot | |
| K864 | Ubiquitination | Uniprot | |
| K921 | Ubiquitination | Uniprot | |
| K959 | Acetylation | Uniprot | |
| Y964 | Phosphorylation | Uniprot | |
| K982 | Ubiquitination | Uniprot | |
| K1032 | Acetylation | Uniprot | |
| K1059 | Ubiquitination | Uniprot | |
| K1068 | Ubiquitination | Uniprot | |
| K1089 | Ubiquitination | Uniprot | |
| T1113 | Phosphorylation | Uniprot | |
| T1114 | Phosphorylation | Uniprot | |
| K1119 | Ubiquitination | Uniprot | |
| Y1125 | Phosphorylation | Uniprot | |
| K1126 | Ubiquitination | Uniprot | |
| K1134 | Ubiquitination | Uniprot | |
| Y1162 | Phosphorylation | Uniprot | |
| K1178 | Ubiquitination | Uniprot | |
| K1190 | Ubiquitination | Uniprot | |
| K1285 | Acetylation | Uniprot | |
| K1285 | Ubiquitination | Uniprot | |
| K1291 | Ubiquitination | Uniprot | |
| K1301 | Ubiquitination | Uniprot |
研究背景
Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11 to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation.
Nucleus. Chromosome>Telomere. Chromosome.
Note: Localizes to discrete nuclear foci after treatment with genotoxic agents.
Expressed at very low level in most tissues, except in testis where it is expressed at higher level. Expressed in fibroblasts.
Component of the MRN complex composed of two heterodimers RAD50/MRE11 associated with a single NBN. As part of the MRN complex, interacts with MCM8 and MCM9; the interaction recruits the complex to DNA repair sites. Component of the BASC complex, at least composed of BRCA1, MSH2, MSH6, MLH1, ATM, BLM, RAD50, MRE11 and NBN. Found in a complex with TERF2. Interacts with RINT1. Interacts with BRCA1 via its N-terminal domain. Interacts with DCLRE1C/Artemis. Interacts with MRNIP.
(Microbial infection) Interacts with herpes simplex virus 1 protein UL12.
The zinc-hook, which separates the large intramolecular coiled coil regions, contains 2 Cys residues that coordinate one molecule of zinc with the help of the 2 Cys residues of the zinc-hook of another RAD50 molecule, thereby forming a V-shaped homodimer. The two heads of the homodimer, which constitute the ATP-binding domain, interact with the MRE11 homodimer (By similarity).
Belongs to the SMC family. RAD50 subfamily.
研究领域
· Cellular Processes > Cell growth and death > Cellular senescence. (View pathway)
· Genetic Information Processing > Replication and repair > Homologous recombination.
· Genetic Information Processing > Replication and repair > Non-homologous end-joining.
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