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产品:	HNF1A 抗体
货号:	DF7180
描述:	Rabbit polyclonal antibody to HNF1A
应用:	WB
反应:	Human, Mouse, Rat
预测:	Zebrafish, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
分子量:	56kDa; 67kD(Calculated).
蛋白号:	P20823
RRID:	AB_2839132

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二抗
封闭肽

阻断肽(封闭肽)是特异性结合目标抗体和阻断抗体结合的多肽。这些多肽包含抗体识别的抗原表位。与阻断肽结合的抗体不再与靶蛋白上的表位结合。例如，在免疫印迹(WB)和免疫组化(IHC)中，通过比较被阻断抗体和未阻断抗体的染色，可以看到哪种染色是特异性的。

规格	价格	库存
50ul	RMB¥ 1250	现货
100ul	RMB¥ 2300	现货
200ul	RMB¥ 3000	现货

货期: 当天发货

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实验方案

产品描述

来源:

Rabbit

应用:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:

Human,Mouse,Rat

预测:

Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(100%), Xenopus(100%)

克隆:

Polyclonal

特异性:

HNF1A Antibody detects endogenous levels of total HNF1A.

RRID:

AB_2839132
引用格式: Affinity Biosciences Cat# DF7180, RRID:AB_2839132.

偶联:

Unconjugated.

纯化:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

别名:

展开/折叠

Albumin proximal factor; Hepatic nuclear factor 1 alpha; Hepatic nuclear factor 1; Hepatic transcription factor 1 alpha; Hepatic transcription factor 1; Hepatocyte nuclear factor 1-alpha; HNF 1; HNF 1A; HNF-1-alpha; HNF-1A; hnf1a; HNF1A_HUMAN; Interferon production regulator factor; LF B1; LF B1 hepatic nuclear factor; LFB 1; LFB1; LFB1 hepatic nuclear factor; Liver specific transcription factor LF B1; Liver specific transcription factor LFB1; Liver-specific transcription factor LF-B1; Maturity onset diabetes of the young 3; MODY 3; MODY3; TCF 1; TCF-1; TCF1; Transcription factor 1; Transcription factor 1 hepatic;

抗原和靶标

免疫原:

Uniprot:

P20823(HNF1A_HUMAN) >>Visit HPA database.

基因/基因ID:

HNF1A(6927)

表达:

P20823 HNF1A_HUMAN:

Liver.

描述:

The protein encoded by this gene is a transcription factor required for the expression of several liver-specific genes. The encoded protein functions as a homodimer and binds to the inverted palindrome 5'-GTTAATNATTAAC-3'. Defects in this gene are a cause of maturity onset diabetes of the young type 3 (MODY3) and also can result in the appearance of hepatic adenomas.

序列:

P20823 HNF1A_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MVSKLSQLQTELLAALLESGLSKEALIQALGEPGPYLLAGEGPLDKGESCGGGRGELAELPNGLGETRGSEDETDDDGEDFTPPILKELENLSPEEAAHQKAVVETLLQEDPWRVAKMVKSYLQQHNIPQREVVDTTGLNQSHLSQHLNKGTPMKTQKRAALYTWYVRKQREVAQQFTHAGQGGLIEEPTGDELPTKKGRRNRFKWGPASQQILFQAYERQKNPSKEERETLVEECNRAECIQRGVSPSQAQGLGSNLVTEVRVYNWFANRRKEEAFRHKLAMDTYSGPPPGPGPGPALPAHSSPGLPPPALSPSKVHGVRYGQPATSETAEVPSSSGGPLVTVSTPLHQVSPTGLEPSHSLLSTEAKLVSAAGGPLPPVSTLTALHSLEQTSPGLNQQPQNLIMASLPGVMTIGPGEPASLGPTFTNTGASTLVIGLASTQAQSVPVINSMGSSLTTLQPVQFSQPLHPSYQQPLMPPVQSHVTQSPFMATMAQLQSPHALYSHKPEVAQYTHTGLLPQTMLITDTTNLSALASLTPTKQVFTSDTEASSESGLHTPASQATTLHVPSQDPAGIQHLQPAHRLSASPTVSSSSLVLYQSSDSSNGQSHLLPSNHSVIETFISTQMASSSQ

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species

Results

Score

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

100

Zebrafish

100

Chicken

100

Pig

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P20823 作为底物

P20823

Site	PTM Type	Enzyme	Source
T67	Phosphorylation		Uniprot
S70	Phosphorylation		Uniprot
T74	Phosphorylation		Uniprot
T82	Phosphorylation		Uniprot
S93	Phosphorylation		Uniprot
T196	Phosphorylation		Uniprot
S247	Phosphorylation		Uniprot
S249	Phosphorylation	Q9Y463 (DYRK1B) , Q13315 (ATM)	Uniprot

研究背景

P20823_HNF1A_HUMAN

功能:

Transcriptional activator that regulates the tissue specific expression of multiple genes, especially in pancreatic islet cells and in liver (By similarity). Binds to the inverted palindrome 5'-GTTAATNATTAAC-3'. Activates the transcription of CYP1A2, CYP2E1 and CYP3A11 (By similarity).

细胞定位:

Nucleus.

组织特异性:

Liver.

亚基结构:

Binds DNA as a dimer. Heterotetramer with PCBD1; formed by a dimer of dimers (By similarity). Interacts with PCBD1. Interacts with BHLHE41 (By similarity).

蛋白家族:

Belongs to the HNF1 homeobox family.

研究领域

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells. (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Maturity onset diabetes of the young.

文献引用

1). SLC25A18 has prognostic value in colorectal cancer and represses Warburg effect and cell proliferation via Wnt signaling. American Journal of Cancer Research, 2020 (PubMed: 32509397) [IF=5.3]

Application: WB Species: human Sample:

Figure 3.| SLC25A18 negatively regulates genes expression related to aerobics glycolysis, cell proliferation, andWnt/β-catenin cascade. (B) Gene set enrichment analysis implied a negative relationship between highly expressed SLC25A18 and the enrichment of CTNNB1 target genes and Wnt/β-catenin Signaling (HALLMARK_ WNT_BETA_CATENIN_SIGNALING, REACTOME_SIGNALING_BY_WNT) in patients with CRC from the TCGA database, (C)which was also verified by western blotting. All experiments were conducted in triplicates.

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HNF1A Antibody - #DF7180

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