(3)
(3)
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF7187, RRID:AB_2839139.
展开/折叠
E3 ubiquitin-protein ligase TRIM63; FLJ32380; IRF; Iris RING finger protein; MURF 1; MURF-1; MuRF1; MURF2; Muscle specific ring finger protein 1; Muscle specific ring finger protein 2; Muscle-specific RING finger protein 1; OTTHUMP00000008701; RING finger protein 28; RNF 28; RNF28; SMRZ; Striated muscle RING zinc finger protein; TRI63_HUMAN; TRIM 63; Trim63; Tripartite motif containing 63; tripartite motif containing 63, E3 ubiquitin protein ligase; Tripartite motif containing protein 63; Tripartite motif-containing protein 63; Ubiquitin ligase TRIM63;
抗原和靶标
Muscle specific. Selectively expressed in heart and skeletal muscle. Also expressed in the iris.
- Q969Q1 TRI63_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDYKSSLIQDGNPMENLEKQLICPICLEMFTKPVVILPCQHNLCRKCANDIFQAANPYWTSRGSSVSMSGGRFRCPTCRHEVIMDRHGVYGLQRNLLVENIIDIYKQECSSRPLQKGSHPMCKEHEDEKINIYCLTCEVPTCSMCKVFGIHKACEVAPLQSVFQGQKTELNNCISMLVAGNDRVQTIITQLEDSRRVTKENSHQVKEELSQKFDTLYAILDEKKSELLQRITQEQEKKLSFIEALIQQYQEQLDKSTKLVETAIQSLDEPGGATFLLTAKQLIKSIVEASKGCQLGKTEQGFENMDFFTLDLEHIADALRAIDFGTDEEEEEFIEEEDQEEEESTEGKEEGHQ
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q969Q1 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S64 | Phosphorylation | Uniprot | |
| S67 | Phosphorylation | Uniprot | |
| S69 | Phosphorylation | Uniprot |
研究背景
E3 ubiquitin ligase. Mediates the ubiquitination and subsequent proteasomal degradation of CKM, GMEB1 and HIBADH. Regulates the proteasomal degradation of muscle proteins under amino acid starvation, where muscle protein is catabolized to provide other organs with amino acids. Inhibits de novo skeletal muscle protein synthesis under amino acid starvation. Regulates proteasomal degradation of cardiac troponin I/TNNI3 and probably of other sarcomeric-associated proteins. May play a role in striated muscle atrophy and hypertrophy by regulating an anti-hypertrophic PKC-mediated signaling pathway. May regulate the organization of myofibrils through TTN in muscle cells.
Cytoplasm. Nucleus. Cytoplasm>Myofibril>Sarcomere>M line. Cytoplasm>Myofibril>Sarcomere>Z line.
Note: Colocalizes with TNNI3 in myocytes (By similarity). Localizes to the M- and Z-lines in skeletal muscle.
Muscle specific. Selectively expressed in heart and skeletal muscle. Also expressed in the iris.
Homodimer. Homooligomer and heterooligomer. Interacts with SUMO2, titin/TTN and GMEB1. Interacts with TRIM54 and probably with TRIM55 and TNNI3. Forms a ternary complex with RACK1 and PRKCE (By similarity). Interacts with CKM.
The RING-type zinc finger mediates interaction with SUMO2 and localization to the nucleus. Also required for the E3 ubiquitin ligase activity (By similarity).
The B box-type zinc finger mediates homodimerization.
文献引用
Application: WB Species: Mouse Sample: GA tissues
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