SLC22A8 Antibody - #DF7196

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产品: SLC22A8 抗体
货号: DF7196
描述: Rabbit polyclonal antibody to SLC22A8
应用: WB IHC
反应: Human, Mouse, Rat
预测: Rabbit
分子量: 60kDa; 60kD(Calculated).
蛋白号: Q8TCC7
RRID: AB_2839148

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:25-1:100
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Rabbit(100%)
克隆:
Polyclonal
特异性:
SLC22A8 Antibody detects endogenous levels of total SLC22A8.
RRID:
AB_2839148
引用格式: Affinity Biosciences Cat# DF7196, RRID:AB_2839148.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

hOAT3; OAT3; Organic anion transporter 3; S22A8_HUMAN; SLC22A8; Solute carrier family 22 (organic anion transporter), member 8; Solute carrier family 22 member 8;

抗原和靶标

免疫原:
Uniprot:

Q8TCC7(S22A8_HUMAN) >>Visit HPA database.

基因/基因ID:
SLC22A8(9376)
表达:
Q8TCC7 S22A8_HUMAN:

Expressed in kidney.

描述:
This gene encodes a protein involved in the sodium-independent transport and excretion of organic anions, some of which are potentially toxic. The encoded protein is an integral membrane protein and appears to be localized to the basolateral membrane of the kidney. Multiple alternatively spliced transcript variants that encode different protein isoforms have been described for this gene.
序列:
MTFSEILDRVGSMGHFQFLHVAILGLPILNMANHNLLQIFTAATPVHHCRPPHNASTGPWVLPMGPNGKPERCLRFVHPPNASLPNDTQRAMEPCLDGWVYNSTKDSIVTEWDLVCNSNKLKEMAQSIFMAGILIGGLVLGDLSDRFGRRPILTCSYLLLAASGSGAAFSPTFPIYMVFRFLCGFGISGITLSTVILNVEWVPTRMRAIMSTALGYCYTFGQFILPGLAYAIPQWRWLQLTVSIPFFVFFLSSWWTPESIRWLVLSGKSSKALKILRRVAVFNGKKEEGERLSLEELKLNLQKEISLAKAKYTASDLFRIPMLRRMTFCLSLAWFATGFAYYSLAMGVEEFGVNLYILQIIFGGVDVPAKFITILSLSYLGRHTTQAAALLLAGGAILALTFVPLDLQTVRTVLAVFGKGCLSSSFSCLFLYTSELYPTVIRQTGMGVSNLWTRVGSMVSPLVKITGEVQPFIPNIIYGITALLGGSAALFLPETLNQPLPETIEDLENWSLRAKKPKQEPEVEKASQRIPLQPHGPGLGSS

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Rabbit
100
Horse
75
Dog
75
Pig
0
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q8TCC7 作为底物

Site PTM Type Enzyme
S4 Phosphorylation
T439 Phosphorylation

研究背景

功能:

Plays an important role in the excretion/detoxification of endogenous and exogenous organic anions, especially from the brain and kidney. Involved in the transport basolateral of steviol, fexofenadine. Transports benzylpenicillin (PCG), estrone-3-sulfate (E1S), cimetidine (CMD), 2,4-dichloro-phenoxyacetate (2,4-D), p-amino-hippurate (PAH), acyclovir (ACV) and ochratoxin (OTA).

细胞定位:

Basolateral cell membrane>Multi-pass membrane protein.
Note: Localizes on the brush border membrane of the choroid epithelial cells. Localizes to the basolateral membrane of the proximal tubular cells. Localizes on the abluminal and possibly, luminal membrane of the brain capillary endothelial cells (BCEC) (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in kidney.

蛋白家族:

Belongs to the major facilitator (TC 2.A.1) superfamily. Organic cation transporter (TC 2.A.1.19) family.

文献引用

1). Caffeic acid phenethyl ester alleviated hypouricemia in hyperuricemic mice through inhibiting XOD and up-regulating OAT3. PHYTOMEDICINE, 2022 (PubMed: 35714456) [IF=7.9]
2). Hypouricemic effect of gallic acid, a bioactive compound from Sonneratia apetala leaves and branches, on hyperuricemic mice. Food & Function, 2022 (PubMed: 36125096) [IF=6.1]
3). Berberrubine attenuates potassium oxonate- and hypoxanthine-induced hyperuricemia by regulating urate transporters and JAK2/STAT3 signaling pathway. European Journal of Pharmacology, 2021 (PubMed: 34699754) [IF=5.0]
4). Xanthones from securidaca inappendiculata antagonized the anti-rheumatic effects of methotrexate in vivo by promoting its secretion into urine. Expert Opinion on Drug Metabolism & Toxicology, 2021 (PubMed: 33107357) [IF=4.3]

Application: WB    Species: rat    Sample: kidney

Figure 4. | XRF promoted MTX secretion into urine in CIA rats.; B, effects of treatments on OAT3 expression in kidney, investigated by the immunoblotting method, **p < 0.01 compared with CIA models, ##p < 0.01 compared with MTX treated rats
5). Hypouricaemic and nephroprotective effects of Poria cocos in hyperuricemic mice by up-regulating ATP-binding cassette super-family G member 2. PHARMACEUTICAL BIOLOGY, 2021 (PubMed: 33651969) [IF=3.8]

Application: WB    Species: Mice    Sample: kidney tissues

Figure 4. Effects of PCE and PCW on renal ABCG2, OAT3, OAT1 and OCT2 protein expression detected by Western blot: immunoreactive bands (a) and densitometries (b,c,d and –e, expressed as mean ± SD; n n ¼ 3).  p < 0.05,  p < 0.01 versus the normal control; #p < 0.05, ##p < 0.01 versus the hyperuricemic control;  p < 0.01 versus the allopurinol control.
6). CP-25 ameliorates methotrexate induced nephrotoxicity via improving renal apoptosis and methotrexate excretion. Journal of Pharmacological Sciences, 2021 (PubMed: 33858651) [IF=3.5]

Application: WB    Species: Rat    Sample: renal tissue

Fig. 4. Effects of CP-25 on renal OAT3 expression and function in vivo. A: the renal excretion of MTX were measured in rats that received a single intravenous injection of MTX (5 mg/kg). B: the tissue extracts were prepared, and the lysate was resolved by SDS-PAGE and probed with antibodies against OAT3. C: OAT3 function was evaluated by detecting DHEA excretion in the rats received a single intravenous injection of DHEA (20 mg/kg). The data are presented as the means ± S.D. (n ¼ 6). ##P < 0.01 compared with normal group; *P < 0.05, **P < 0.01 compared with the model group (MTX).

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