产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF7199, RRID:AB_2839151.
展开/折叠
PP2A A; PP2A alpha; PP2A B; PP2A beta; PP2A-alpha; PP2AA_HUMAN; PP2Ac; PP2CB; PPP2CA; PPP2CB; Protein phosphatase 2 catalytic subunit alpha isoform; Protein phosphatase 2 catalytic subunit beta isoform; Replication protein C; RP C; RP-C; Serine/threonine protein phosphatase 2A catalytic subunit alpha isoform; Serine/threonine protein phosphatase 2A catalytic subunit beta isoform; Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform;
抗原和靶标
- P62714 PP2AB_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDDKAFTKELDQWVEQLNECKQLNENQVRTLCEKAKEILTKESNVQEVRCPVTVCGDVHGQFHDLMELFRIGGKSPDTNYLFMGDYVDRGYYSVETVTLLVALKVRYPERITILRGNHESRQITQVYGFYDECLRKYGNANVWKYFTDLFDYLPLTALVDGQIFCLHGGLSPSIDTLDHIRALDRLQEVPHEGPMCDLLWSDPDDRGGWGISPRGAGYTFGQDISETFNHANGLTLVSRAHQLVMEGYNWCHDRNVVTIFSAPNYCYRCGNQAAIMELDDTLKYSFLQFDPAPRRGEPHVTRRTPDYFL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P62714 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K4 | Ubiquitination | Uniprot | |
| K8 | Ubiquitination | Uniprot | |
| K21 | Ubiquitination | Uniprot | |
| K34 | Ubiquitination | Uniprot | |
| K36 | Ubiquitination | Uniprot | |
| K41 | Acetylation | Uniprot | |
| K41 | Ubiquitination | Uniprot | |
| K74 | Ubiquitination | Uniprot | |
| S75 | Phosphorylation | Uniprot | |
| T78 | Phosphorylation | Uniprot | |
| Y86 | Phosphorylation | Uniprot | |
| K136 | Ubiquitination | Uniprot | |
| S212 | Phosphorylation | Uniprot | |
| Y248 | Phosphorylation | Uniprot | |
| Y265 | Phosphorylation | Uniprot | |
| K283 | Ubiquitination | Uniprot | |
| Y284 | Phosphorylation | Uniprot | |
| R295 | Methylation | Uniprot | |
| T304 | Phosphorylation | Uniprot | |
| Y307 | Phosphorylation | Uniprot |
研究背景
PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase.
Reversibly methyl esterified on Leu-309 by leucine carboxyl methyltransferase 1 (LCMT1) and protein phosphatase methylesterase 1 (PPME1). Carboxyl methylation influences the affinity of the catalytic subunit for the different regulatory subunits, thereby modulating the PP2A holoenzyme's substrate specificity, enzyme activity and cellular localization.
Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation.
May be monoubiquitinated by NOSIP.
Cytoplasm. Nucleus. Chromosome>Centromere. Cytoplasm>Cytoskeleton>Spindle pole.
Note: In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles.
Found in a complex with at least ARL2, PPP2CB, PPP2R1A, PPP2R2A, PPP2R5E and TBCD. Interacts with TBCD (By similarity). PP2A consists of a common heterodimeric core enzyme (composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65) (subunit A)) that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Binds PPME1. May indirectly interact with SGO1, most probably through regulatory B56 subunits. Interacts with CTTNBP2NL. Interacts with PTPA.
Belongs to the PPP phosphatase family. PP-1 subfamily.
研究领域
· Cellular Processes > Cell growth and death > Oocyte meiosis. (View pathway)
· Cellular Processes > Transport and catabolism > Autophagy - other. (View pathway)
· Cellular Processes > Transport and catabolism > Autophagy - animal. (View pathway)
· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)
· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > AMPK signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > TGF-beta signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Hippo signaling pathway. (View pathway)
· Genetic Information Processing > Translation > mRNA surveillance pathway.
· Human Diseases > Infectious diseases: Parasitic > Chagas disease (American trypanosomiasis).
· Human Diseases > Infectious diseases: Viral > Hepatitis C.
· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.
· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes. (View pathway)
· Organismal Systems > Nervous system > Dopaminergic synapse.
· Organismal Systems > Nervous system > Long-term depression.
限制条款
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