| 产品: | RAD23A 抗体 |
| 货号: | DF7241 |
| 描述: | Rabbit polyclonal antibody to RAD23A |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Horse, Sheep, Dog, Chicken |
| 分子量: | 39kDa,54kDa; 40kD(Calculated). |
| 蛋白号: | P54725 |
| RRID: | AB_2839180 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF7241, RRID:AB_2839180.
展开/折叠
hHR 23A; hHR23A; HR23A; MGC111083; RAD 23a; RAD23 homolog A (S. cerevisiae); RAD23 homolog A; RAD23 yeast homolog A; RAD23A; RD23A_HUMAN; UV excision repair protein RAD23; UV excision repair protein RAD23 homolog A;
抗原和靶标
- P54725 RD23A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAVTITLKTLQQQTFKIRMEPDETVKVLKEKIEAEKGRDAFPVAGQKLIYAGKILSDDVPIRDYRIDEKNFVVVMVTKTKAGQGTSAPPEASPTAAPESSTSFPPAPTSGMSHPPPAAREDKSPSEESAPTTSPESVSGSVPSSGSSGREEDAASTLVTGSEYETMLTEIMSMGYERERVVAALRASYNNPHRAVEYLLTGIPGSPEPEHGSVQESQVSEQPATEAAGENPLEFLRDQPQFQNMRQVIQQNPALLPALLQQLGQENPQLLQQISRHQEQFIQMLNEPPGELADISDVEGEVGAIGEEAPQMNYIQVTPQEKEAIERLKALGFPESLVIQAYFACEKNENLAANFLLSQNFDDE
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P54725 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K8 | Ubiquitination | Uniprot | |
| K16 | Acetylation | Uniprot | |
| K16 | Ubiquitination | Uniprot | |
| K26 | Ubiquitination | Uniprot | |
| K29 | Ubiquitination | Uniprot | |
| K31 | Ubiquitination | Uniprot | |
| K36 | Ubiquitination | Uniprot | |
| K47 | Acetylation | Uniprot | |
| K47 | Ubiquitination | Uniprot | |
| K53 | Acetylation | Uniprot | |
| K53 | Ubiquitination | Uniprot | |
| S56 | Phosphorylation | Uniprot | |
| K69 | Ubiquitination | Uniprot | |
| K78 | Ubiquitination | Uniprot | |
| K80 | Ubiquitination | Uniprot | |
| S92 | Phosphorylation | Uniprot | |
| T94 | Phosphorylation | Uniprot | |
| K122 | Ubiquitination | Uniprot | |
| S123 | Phosphorylation | Uniprot | |
| S125 | Phosphorylation | Uniprot | |
| S128 | Phosphorylation | Uniprot | |
| T132 | Phosphorylation | Uniprot | |
| S133 | Phosphorylation | Uniprot | |
| S136 | Phosphorylation | Uniprot | |
| S138 | Phosphorylation | Uniprot | |
| S143 | Phosphorylation | Uniprot | |
| S144 | Phosphorylation | Uniprot | |
| S147 | Phosphorylation | Uniprot | |
| Y163 | Phosphorylation | Uniprot | |
| Y197 | Phosphorylation | Uniprot | |
| S205 | Phosphorylation | Uniprot | |
| S212 | Phosphorylation | Uniprot | |
| S216 | Phosphorylation | Uniprot | |
| S274 | Phosphorylation | Uniprot | |
| S295 | Phosphorylation | Uniprot | |
| Y313 | Phosphorylation | Uniprot | |
| K321 | Ubiquitination | Uniprot | |
| S357 | Phosphorylation | Uniprot |
研究背景
Multiubiquitin chain receptor involved in modulation of proteasomal degradation. Binds to 'Lys-48'-linked polyubiquitin chains in a length-dependent manner and with a lower affinity to 'Lys-63'-linked polyubiquitin chains. Proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome.
Involved in nucleotide excision repair and is thought to be functional equivalent for RAD23B in global genome nucleotide excision repair (GG-NER) by association with XPC. In vitro, the XPC:RAD23A dimer has NER activity. Can stabilize XPC.
(Microbial infection) Involved in Vpr-dependent replication of HIV-1 in non-proliferating cells and primary macrophages. Required for the association of HIV-1 Vpr with the host proteasome.
Nucleus.
Interacts with XPC; the interaction is suggesting the existence of a functional equivalent variant XPC complex. Interacts with PSMD4 and PSMC5. Interacts with ATXN3. Interacts with UBQLN2.
(Microbial infection) Interacts with HIV-1 Vpr.
The ubiquitin-like domain mediates interaction with ATXN3.
The ubiquitin-like (UBL) and the UBA (ubiquitin-associated) domains interact intramolecularly in a highly dynamic manner, as each UBA domain competes for an overlapping UBL domain surface. Binding of ubiquitin or proteasome subunit PSMD4 disrupt the UBL-UBA domain interactions and drive RAD23A in to an open conformation.
Belongs to the RAD23 family.
研究领域
· Genetic Information Processing > Replication and repair > Nucleotide excision repair.
· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)
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