ERCC1 Antibody | Affinity Biosciences LTD|亲科生物官网

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Western blot analysis of Hela whole cell lysates, using ERCC1 Antibody.

DF7255 at 1/100 staining Human liver tissue by IHC-P.

产品:	ERCC1 抗体
货号:	DF7255
描述:	Rabbit polyclonal antibody to ERCC1
应用:	WB IHC IF/ICC
反应:	Human, Mouse, Rat
预测:	Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Xenopus
分子量:	33kDa; 33kD(Calculated).
蛋白号:	P07992
RRID:	AB_2839194

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阻断肽(封闭肽)是特异性结合目标抗体和阻断抗体结合的多肽。这些多肽包含抗体识别的抗原表位。与阻断肽结合的抗体不再与靶蛋白上的表位结合。例如，在免疫印迹(WB)和免疫组化(IHC)中，通过比较被阻断抗体和未阻断抗体的染色，可以看到哪种染色是特异性的。

规格	价格	库存
50ul	RMB¥ 1250	现货
100ul	RMB¥ 2300	现货
200ul	RMB¥ 3000	现货

货期: 当天发货

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实验方案

产品描述

来源:

Rabbit

应用:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:

Human,Mouse,Rat

预测:

Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Xenopus(100%)

克隆:

Polyclonal

特异性:

ERCC1 Antibody detects endogenous levels of total ERCC1.

RRID:

AB_2839194
引用格式: Affinity Biosciences Cat# DF7255, RRID:AB_2839194.

偶联:

Unconjugated.

纯化:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

别名:

展开/折叠

COFS 4; COFS4; DNA excision repair protein ERCC 1; DNA excision repair protein ERCC-1; DNA excision repair protein ERCC1; ERCC 1; ERCC1; ERCC1_HUMAN; Excision repair cross complementation group 1; Excision repair cross complementing 1; Excision Repair Cross Complementing Rodent Repair Deficiency Complementation Group 1; Excision repair protein; RAD 10; RAD10; UV 20; UV20;

抗原和靶标

免疫原:

Uniprot:

P07992(ERCC1_HUMAN) >>Visit HPA database.

基因/基因ID:

ERCC1(2067)

描述:

The product of this gene functions in the nucleotide excision repair pathway, and is required for the repair of DNA lesions such as those induced by UV light or formed by electrophilic compounds including cisplatin. The encoded protein forms a heterodimer with the XPF endonuclease (also known as ERCC4), and the heterodimeric endonuclease catalyzes the 5' incision in the process of excising the DNA lesion. The heterodimeric endonuclease is also involved in recombinational DNA repair and in the repair of inter-strand crosslinks. Mutations in this gene result in cerebrooculofacioskeletal syndrome, and polymorphisms that alter expression of this gene may play a role in carcinogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. The last exon of this gene overlaps with the CD3e molecule, epsilon associated protein gene on the opposite strand.

序列:

P07992 ERCC1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDPGKDKEGVPQPSGPPARKKFVIPLDEDEVPPGVAKPLFRSTQSLPTVDTSAQAAPQTYAEYAISQPLEGAGATCPTGSEPLAGETPNQALKPGAKSNSIIVSPRQRGNPVLKFVRNVPWEFGDVIPDYVLGQSTCALFLSLRYHNLHPDYIHGRLQSLGKNFALRVLLVQVDVKDPQQALKELAKMCILADCTLILAWSPEEAGRYLETYKAYEQKPADLLMEKLEQDFVSRVTECLTTVKSVNKTDSQTLLTTFGSLEQLIAASREDLALCPGLGPQKARRLFDVLHEPFLKVP

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

100

Zebrafish

100

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P07992 作为底物

P07992

Site	PTM Type	Source
	Ubiquitination	Uniprot
M1	Acetylation	Uniprot
S14	Phosphorylation	Uniprot
K21	Ubiquitination	Uniprot
K37	Acetylation	Uniprot
K37	Ubiquitination	Uniprot
S104	Phosphorylation	Uniprot
K114	Ubiquitination	Uniprot
K162	Ubiquitination	Uniprot
K213	Ubiquitination	Uniprot
K226	Ubiquitination	Uniprot
K243	Ubiquitination	Uniprot
K247	Ubiquitination	Uniprot
K281	Ubiquitination	Uniprot
K295	Acetylation	Uniprot
K295	Ubiquitination	Uniprot

研究背景

P07992_ERCC1_HUMAN

功能:

Non-catalytic component of a structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair. Responsible, in conjunction with SLX4, for the first step in the repair of interstrand cross-links (ICL). Participates in the processing of anaphase bridge-generating DNA structures, which consist in incompletely processed DNA lesions arising during S or G2 phase, and can result in cytokinesis failure. Also required for homology-directed repair (HDR) of DNA double-strand breaks, in conjunction with SLX4.

细胞定位:

Nucleus.

Cytoplasm. Nucleus.

Nucleus.

Nucleus.

亚基结构:

Heterodimer composed of ERCC1 isoform 1 and XPF/ERRC4.

蛋白家族:

Belongs to the ERCC1/RAD10/SWI10 family.

研究领域

· Genetic Information Processing > Replication and repair > Nucleotide excision repair.

· Genetic Information Processing > Replication and repair > Fanconi anemia pathway.

· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.

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ERCC1 Antibody - #DF7255

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