GCLM Antibody | Affinity Biosciences LTD|亲科生物官网

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Figure 3: Effects of oregano essential oil (OEO) on g-glutamylcysteine ligase (GCLM, GCLC), and Cu/Zn-superoxide dismutase (SOD1) expression in IPEC-J2 cells.

Figure 9.

FIGURE 5 | Expression of the targeted proteins in mice liver.

Fig.

Figure 6.

Fig.

产品:	GCLM 抗体
货号:	DF7268
描述:	Rabbit polyclonal antibody to GCLM
应用:	WB IHC
反应:	Human, Mouse, Rat, Pig
预测:	Pig, Bovine, Rabbit
分子量:	30kDa; 31kD(Calculated).
蛋白号:	P48507
RRID:	AB_2839207

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阻断肽(封闭肽)是特异性结合目标抗体和阻断抗体结合的多肽。这些多肽包含抗体识别的抗原表位。与阻断肽结合的抗体不再与靶蛋白上的表位结合。例如，在免疫印迹(WB)和免疫组化(IHC)中，通过比较被阻断抗体和未阻断抗体的染色，可以看到哪种染色是特异性的。

规格	价格	库存
50ul	RMB¥ 1250	现货
100ul	RMB¥ 2300	现货
200ul	RMB¥ 3000	现货

货期: 当天发货

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实验方案

产品描述

来源:

Rabbit

应用:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:

Human,Mouse,Rat,Pig

预测:

Bovine(100%), Rabbit(100%)

克隆:

Polyclonal

特异性:

GCLM Antibody detects endogenous levels of total GCLM.

RRID:

AB_2839207
引用格式: Affinity Biosciences Cat# DF7268, RRID:AB_2839207.

偶联:

Unconjugated.

纯化:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

别名:

展开/折叠

Gamma ECS regulatory subunit; Gamma-ECS regulatory subunit; Gamma-glutamylcysteine synthetase regulatory subunit; GCLM; GCS light chain; GLCLR; Glutamate cysteine ligase regulatory subunit; Glutamate--cysteine ligase modifier subunit; Glutamate--cysteine ligase regulatory subunit; GSC light chain; GSH0_HUMAN;

抗原和靶标

免疫原:

Uniprot:

P48507(GSH0_HUMAN) >>Visit HPA database.

基因/基因ID:

GCLM(2730)

表达:

P48507 GSH0_HUMAN:

In all tissues examined. Highest levels in skeletal muscle.

描述:

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia.

序列:

P48507 GSH0_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGTDSRAAKALLARARTLHLQTGNLLNWGRLRKKCPSTHSEELHDCIQKTLNEWSSQINPDLVREFPDVLECTVSHAVEKINPDEREEMKVSAKLFIVESNSSSSTRSAVDMACSVLGVAQLDSVIIASPPIEDGVNLSLEHLQPYWEELENLVQSKKIVAIGTSDLDKTQLEQLYQWAQVKPNSNQVNLASCCVMPPDLTAFAKQFDIQLLTHNDPKELLSEASFQEALQESIPDIQAHEWVPLWLLRYSVIVKSRGIIKSKGYILQAKRRGS

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species

Results

Score

Pig

100

Bovine

100

Rabbit

100

Xenopus

Horse

Sheep

Dog

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P48507 作为底物

P48507

Site	PTM Type	Source
K9	Sumoylation	Uniprot
K9	Ubiquitination	Uniprot
T17	Phosphorylation	Uniprot
K34	Ubiquitination	Uniprot
C35	S-Nitrosylation	Uniprot
K49	Ubiquitination	Uniprot
C72	S-Nitrosylation	Uniprot
K80	Ubiquitination	Uniprot
K90	Ubiquitination	Uniprot
K158	Sumoylation	Uniprot
K158	Ubiquitination	Uniprot
T164	Phosphorylation	Uniprot
S165	Phosphorylation	Uniprot
K169	Ubiquitination	Uniprot
K205	Sumoylation	Uniprot
K263	Acetylation	Uniprot
K263	Ubiquitination	Uniprot
Y265	Phosphorylation	Uniprot
K270	Ubiquitination	Uniprot

研究背景

P48507_GSH0_HUMAN

组织特异性:

In all tissues examined. Highest levels in skeletal muscle.

亚基结构:

Heterodimer of a catalytic heavy chain and a regulatory light chain.

蛋白家族:

Belongs to the aldo/keto reductase family. Glutamate--cysteine ligase light chain subfamily.

研究领域

· Cellular Processes > Cell growth and death > Ferroptosis. (View pathway)

· Metabolism > Amino acid metabolism > Cysteine and methionine metabolism.

· Metabolism > Metabolism of other amino acids > Glutathione metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

文献引用

1). Gly-Pro-Ala peptide and FGSHF3 exert protective effects in DON-induced toxicity and intestinal damage via decreasing oxidative stress. FOOD RESEARCH INTERNATIONAL, 2021 (PubMed: 33509464) [IF=8.1]

2). Total flavonoids of Inula japonica alleviated the inflammatory response and oxidative stress in LPS-induced acute lung injury via inhibiting the sEH activity: Insights from lipid metabolomics. PHYTOMEDICINE, 2022 (PubMed: 36150346) [IF=7.9]

3). Itaconate inhibits ferroptosis of macrophage via Nrf2 pathways against sepsis-induced acute lung injury. Cell Death Discovery, 2022 (PubMed: 35110526) [IF=7.0]

Application: WB Species: Mice Sample: lung tissues

Fig. 3 4-OI inhibits ferroptosis through increasing and activating Nrf2 in vivo during ALI. A Western blots for Nrf2, GCLM, HO-1 and SLC7A11 in murine lung tissue. B–E Relative mRNA levels of Nrf2, GCLM, HO-1 and SLC7A11 in murine lung tissue (n = 6). F, G Relative levels of GSH and GSH/GSSG ratio in murine lung tissue (n = 6). H, I Representative images of fluorescence probe for ROS and its statistical results (n = 6) in lung tissue. (Data are presented as Mean ± SD *p < 0.05, **p < 0.01, ***p < 0.001).

4). Ameliorate effect of pyrroloquinoline quinone against cyclophosphamide-induced nephrotoxicity by activating the Nrf2 pathway and inhibiting the NLRP3 pathway. LIFE SCIENCES, 2020 (PubMed: 32504759) [IF=6.1]

Application: WB Species: Mice Sample: kidney tissues

Fig. 6. Effect of PQQ on protein expression in the Nrf2/HO-1 pathway. The expression levels of Nrf2 (A), HO-1 (B), GCLM (C), and NQO1 (D). Data are shown as mean ± SD, n = 8. #p < 0.05 and ##p < 0.01 versus the Control group. ⁎ p < 0.05 and ⁎⁎p < 0.01 versus the Model group.

5). Therapeutic role of D-pinitol on experimental colitis via activating Nrf2/ARE and PPAR-γ/NF-κB signaling pathways. Food & Function, 2021 (PubMed: 33625409) [IF=6.1]

6). Pyrroloquinoline quinone ameliorates liver injury in mice induced by cyclophosphamide. Environmental Science and Pollution Research, 2022 (PubMed: 34997497) [IF=5.8]

7). Curc-mPEG454, a PEGylated curcumin derivative, as a multi-target anti-fibrotic prodrug. International immunopharmacology, 2021 (PubMed: 34628270) [IF=5.6]

Application: WB Species: Rat Sample: liver tissues

Fig. 6. Curc-mPEG454 up-regulates GCLC and GCLM for GSH de novo synthesis. (A) Heatmap represents gene expression of Glutathione metabolism. Gene related to glutathione synthesis and metabolism are indicated. (B) Paired display the gene expression by RNA-seq and relative mRNA expression by qRT-PCR from glutathione synthesis and metabolism. Gene expression level was measured in FPKM value. The mRNA levels were normalized to GAPDH, and subsequently represents as fold change relative to control group. (C) The protein levels of NRF2, GCLC and GCLM. (D) Curc-mPEG454-100 mg significantly restores GSH level during liver fibrosis. Contents of GSH in liver tissues were detected at the 6th week. Data are expressed as mean ± SEM. #P < 0.05, ##P < 0.01, ###P < 0.001 compared with normal group. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 compared with CCl4-induced model group.

8). Hepatoprotective and Anti-Oxidative Effects of Total Flavonoids From Qu Zhi Qiao (Fruit of Citrus Paradisi cv.Changshanhuyou) on Nonalcoholic Steatohepatitis In Vivo and In Vitro Through Nrf2-ARE Signaling Pathway. Frontiers in Pharmacology, 2020 (PubMed: 32390839) [IF=5.6]

Application: WB Species: mouse Sample: liver

FIGURE 5 | Expression of the targeted proteins in mice liver. Values are shown as mean ± SD (n = 8). Data (mean ± SD) with different case letters are statistically different with each other at LSD multiple comparisons, which with red letters differed significantly as compared to model group level. uppercase letter p < 0.01 vs.another group.

9). Novel amphibian-derived antioxidant peptide protects skin against ultraviolet irradiation damage. Journal of photochemistry and photobiology-B, Biology, 2021 (PubMed: 34628205) [IF=5.4]

10). Sodium Danshensu Cream Promotes the Healing of Pressure Ulcers in Mice through the Nrf2/HO-1 and NF-κB Pathways. Pharmaceuticals, 2022 (PubMed: 36558999) [IF=4.6]

Application: WB Species: Mouse Sample:

Figure 6. Effect of SDSS on the protein levels of the Nrf2/HO-1 pathway (n = 5). (A) Western blot; the protein expression levels of Keap1 (B), Nrf2 (C), HO-1 (D), NQO1 (E), and GCLM (F) levels. * p < 0.05, ** p < 0.01 when compared to the control group. # p < 0.05, ## p < 0.01 when compared to the positive group.

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GCLM Antibody - #DF7268

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