| 产品: | SLC4A4 抗体 |
| 货号: | DF7280 |
| 描述: | Rabbit polyclonal antibody to SLC4A4 |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 121kDa; 121kD(Calculated). |
| 蛋白号: | Q9Y6R1 |
| RRID: | AB_2839219 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF7280, RRID:AB_2839219.
展开/折叠
DKFZp781H1314; Electrogenic sodium bicarbonate cotransporter 1; hhNMC; HNBC 1; HNBC1; kNBC 1; KNBC; kNBC1; Na(+)/HCO3(-) cotransporter; Na+HCO3- cotransporter 4; NBC 1; NBC 2; NBC1; NBC2; Nbc4; NBCE 1; NBCE1; OTTHUMP00000160355; OTTHUMP00000218884; OTTHUMP00000218885; PNBC; S4A4_HUMAN; SLC4A4; SLC4A5; Sodium bicarbonate cotransporter 1 (sodium bicarbonate cotransporter, kidney; sodium bicarbonate cotransporter, pancreas); Sodium bicarbonate cotransporter; Sodium bicarbonate cotransporter kidney; sodium bicarbonate cotransporter member 4; Sodium bicarbonate cotransporter pancreas; Solute carrier family 4 member 4; Solute carrier family 4 sodium bicarbonate cotransporter member 4; Solute carrier family 4 sodium bicarbonate cotransporter member 5; Solute carrier family 4, sodium bicarbonate cotransporter, member 4, brain type;
抗原和靶标
Isoform 1 is expressed in pancreas and to a lower extent in heart, skeletal muscle, liver, parotid salivary glands, prostate, colon, stomach, thyroid, brain and spinal chord. Corneal endothelium cells express only isoform 1 (at protein level). Isoform 2 is specifically expressed in kidney at the level of proximal tubules.
- Q9Y6R1 S4A4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEDEAVLDRGASFLKHVCDEEEVEGHHTIYIGVHVPKSYRRRRRHKRKTGHKEKKEKERISENYSDKSDIENADESSSSILKPLISPAAERIRFILGEEDDSPAPPQLFTELDELLAVDGQEMEWKETARWIKFEEKVEQGGERWSKPHVATLSLHSLFELRTCMEKGSIMLDREASSLPQLVEMIVDHQIETGLLKPELKDKVTYTLLRKHRHQTKKSNLRSLADIGKTVSSASRMFTNPDNGSPAMTHRNLTSSSLNDISDKPEKDQLKNKFMKKLPRDAEASNVLVGEVDFLDTPFIAFVRLQQAVMLGALTEVPVPTRFLFILLGPKGKAKSYHEIGRAIATLMSDEVFHDIAYKAKDRHDLIAGIDEFLDEVIVLPPGEWDPAIRIEPPKSLPSSDKRKNMYSGGENVQMNGDTPHDGGHGGGGHGDCEELQRTGRFCGGLIKDIKRKAPFFASDFYDALNIQALSAILFIYLATVTNAITFGGLLGDATDNMQGVLESFLGTAVSGAIFCLFAGQPLTILSSTGPVLVFERLLFNFSKDNNFDYLEFRLWIGLWSAFLCLILVATDASFLVQYFTRFTEEGFSSLISFIFIYDAFKKMIKLADYYPINSNFKVGYNTLFSCTCVPPDPANISISNDTTLAPEYLPTMSSTDMYHNTTFDWAFLSKKECSKYGGNLVGNNCNFVPDITLMSFILFLGTYTSSMALKKFKTSPYFPTTARKLISDFAIILSILIFCVIDALVGVDTPKLIVPSEFKPTSPNRGWFVPPFGENPWWVCLAAAIPALLVTILIFMDQQITAVIVNRKEHKLKKGAGYHLDLFWVAILMVICSLMALPWYVAATVISIAHIDSLKMETETSAPGEQPKFLGVREQRVTGTLVFILTGLSVFMAPILKFIPMPVLYGVFLYMGVASLNGVQFMDRLKLLLMPLKHQPDFIYLRHVPLRRVHLFTFLQVLCLALLWILKSTVAAIIFPVMILALVAVRKGMDYLFSQHDLSFLDDVIPEKDKKKKEDEKKKKKKKGSLDSDNDDSDCPYSEKVPSIKIPMDIMEQQPFLSDSKPSDRERSPTFLERHTSC
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9Y6R1 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S12 | Phosphorylation | Uniprot | |
| T28 | Phosphorylation | Uniprot | |
| Y30 | Phosphorylation | Uniprot | |
| T49 | Phosphorylation | Q9UEW8 (STK39) | Uniprot |
| S61 | Phosphorylation | Uniprot | |
| Y64 | Phosphorylation | Uniprot | |
| S65 | Phosphorylation | Q9UEW8 (STK39) | Uniprot |
| S68 | Phosphorylation | Uniprot | |
| K167 | Acetylation | Uniprot | |
| Y206 | Phosphorylation | Uniprot | |
| T207 | Phosphorylation | Uniprot | |
| S223 | Phosphorylation | Uniprot | |
| K229 | Ubiquitination | Uniprot | |
| S232 | Phosphorylation | Uniprot | |
| S233 | Phosphorylation | Uniprot | |
| S235 | Phosphorylation | Uniprot | |
| T239 | Phosphorylation | Uniprot | |
| S245 | Phosphorylation | Uniprot | |
| T249 | Phosphorylation | Uniprot | |
| T254 | Phosphorylation | Uniprot | |
| S255 | Phosphorylation | Uniprot | |
| S256 | Phosphorylation | Uniprot | |
| S257 | Phosphorylation | Uniprot | |
| S396 | Phosphorylation | Uniprot | |
| Y407 | Phosphorylation | Uniprot | |
| N641 | N-Glycosylation | Uniprot | |
| N661 | N-Glycosylation | Uniprot | |
| Y941 | Phosphorylation | Uniprot | |
| S1026 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| S1029 | Phosphorylation | Uniprot | |
| S1034 | Phosphorylation | Uniprot | |
| Y1038 | Phosphorylation | Uniprot | |
| S1044 | Phosphorylation | Uniprot | |
| S1061 | Phosphorylation | Uniprot | |
| S1064 | Phosphorylation | Uniprot | |
| S1069 | Phosphorylation | Uniprot | |
| T1071 | Phosphorylation | Uniprot | |
| T1077 | Phosphorylation | Uniprot | |
| S1078 | Phosphorylation | Uniprot |
研究背景
Electrogenic sodium/bicarbonate cotransporter with a Na(+):HCO3(-) stoichiometry varying from 1:2 to 1:3. May regulate bicarbonate influx/efflux at the basolateral membrane of cells and regulate intracellular pH.
May have a higher activity than isoform 1.
Phosphorylation of Ser-1026 by PKA increases the binding of CA2 and changes the Na(+):HCO3(-) stoichiometry of the transporter from 3:1 to 2:1. Phosphorylated in presence of STK39 and dephosphorylated in presence of PP1 phosphatase; phosphorylation seems to inhibit SLC4A4 activity.
N-glycosylation is not necessary for the transporter basic functions.
Basolateral cell membrane>Multi-pass membrane protein.
Isoform 1 is expressed in pancreas and to a lower extent in heart, skeletal muscle, liver, parotid salivary glands, prostate, colon, stomach, thyroid, brain and spinal chord. Corneal endothelium cells express only isoform 1 (at protein level). Isoform 2 is specifically expressed in kidney at the level of proximal tubules.
Homodimer. Interacts with CA2/carbonic anhydrase 2 and CA4/carbonic anhydrase 4 which may regulate transporter activity. Isoform 1 but not isoform 2 interacts with AHCYL1 (via PEST domain when phosphorylated); the interaction increases SLC4A4 isoform 1 activity. Interacts with AHCYL2 (By similarity).
Belongs to the anion exchanger (TC 2.A.31) family.
研究领域
· Organismal Systems > Excretory system > Proximal tubule bicarbonate reclamation.
· Organismal Systems > Digestive system > Pancreatic secretion.
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