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产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF7301, RRID:AB_2839239.
展开/折叠
BAF57; BRG1 associated factor 57; BRG1-associated factor 57; Chromatin remodeling complex BRG1 associated factor 57; FLJ35648; SMARCE 1; SMARCE1; SMCE1_HUMAN; SWI/SNF related matrix associated actin dependent regulator of chromatin e1; SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily e member 1; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1;
抗原和靶标
- Q969G3 SMCE1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSKRPSYAPPPTPAPATQMPSTPGFVGYNPYSHLAYNNYRLGGNPGTNSRVTASSGITIPKPPKPPDKPLMPYMRYSRKVWDQVKASNPDLKLWEIGKIIGGMWRDLTDEEKQEYLNEYEAEKIEYNESMKAYHNSPAYLAYINAKSRAEAALEEESRQRQSRMEKGEPYMSIQPAEDPDDYDDGFSMKHTATARFQRNHRLISEILSESVVPDVRSVVTTARMQVLKRQVQSLMVHQRKLEAELLQIEERHQEKKRKFLESTDSFNNELKRLCGLKVEVDMEKIAAEIAQAEEQARKRQEEREKEAAEQAERSQSSIVPEEEQAANKGEEKKDDENIPMETEETHLEETTESQQNGEEGTSTPEDKESGQEGVDSMAEEGTSDSNTGSESNSATVEEPPTDPIPEDEKKE
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q969G3 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K3 | Acetylation | Uniprot | |
| K3 | Methylation | Uniprot | |
| R4 | Methylation | Uniprot | |
| S6 | Phosphorylation | Uniprot | |
| Y7 | Phosphorylation | Uniprot | |
| T12 | Phosphorylation | Uniprot | |
| T17 | Phosphorylation | Uniprot | |
| S21 | Phosphorylation | Uniprot | |
| T22 | Phosphorylation | Uniprot | |
| Y28 | Phosphorylation | Uniprot | |
| Y31 | Phosphorylation | Uniprot | |
| S32 | Phosphorylation | Uniprot | |
| R40 | Methylation | Uniprot | |
| T47 | Phosphorylation | Uniprot | |
| T58 | Phosphorylation | Uniprot | |
| Y73 | Phosphorylation | Uniprot | |
| K85 | Ubiquitination | Uniprot | |
| K92 | Sumoylation | Uniprot | |
| K92 | Ubiquitination | Uniprot | |
| K98 | Ubiquitination | Uniprot | |
| K112 | Ubiquitination | Uniprot | |
| Y115 | Phosphorylation | Uniprot | |
| K131 | Ubiquitination | Uniprot | |
| S136 | Phosphorylation | Uniprot | |
| Y139 | Phosphorylation | Uniprot | |
| Y142 | Phosphorylation | Uniprot | |
| K146 | Ubiquitination | Uniprot | |
| S157 | Phosphorylation | Uniprot | |
| K166 | Ubiquitination | Uniprot | |
| Y170 | Phosphorylation | Uniprot | |
| K189 | Ubiquitination | Uniprot | |
| T191 | Phosphorylation | Uniprot | |
| T193 | Phosphorylation | Uniprot | |
| S204 | Phosphorylation | Uniprot | |
| K228 | Methylation | Uniprot | |
| K240 | Ubiquitination | Uniprot | |
| K258 | Ubiquitination | Uniprot | |
| T263 | Phosphorylation | Uniprot | |
| S265 | Phosphorylation | Uniprot | |
| K271 | Ubiquitination | Uniprot | |
| K277 | Sumoylation | Uniprot | |
| K277 | Ubiquitination | Uniprot | |
| K284 | Ubiquitination | Uniprot | |
| S314 | Phosphorylation | Uniprot | |
| S317 | Phosphorylation | Uniprot | |
| K328 | Acetylation | Uniprot | |
| K328 | Ubiquitination | Uniprot | |
| T350 | Phosphorylation | Uniprot | |
| S353 | Phosphorylation | Uniprot | |
| S362 | Phosphorylation | Uniprot | |
| T363 | Phosphorylation | Uniprot |
研究背景
Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity). Required for the coactivation of estrogen responsive promoters by SWI/SNF complexes and the SRC/p160 family of histone acetyltransferases (HATs). Also specifically interacts with the CoREST corepressor resulting in repression of neuronal specific gene promoters in non-neuronal cells.
Ubiquitinated by TRIP12, leading to its degradation by the proteasome. Ubiquitination is prevented upon interaction between TRIP12 and SMARCC1.
Nucleus.
Component of the multiprotein chromatin-remodeling complexes SWI/SNF: SWI/SNF-A (BAF), SWI/SNF-B (PBAF) and related complexes. The canonical complex contains a catalytic subunit (either SMARCA4/BRG1/BAF190A or SMARCA2/BRM/BAF190B), and at least SMARCE1, ACTL6A/BAF53, SMARCC1/BAF155, SMARCC2/BAF170, and SMARCB1/SNF5/BAF47. Other subunits specific to each of the complexes may also be present permitting several possible combinations developmentally and tissue specific. Component of the BAF complex, which includes at least actin (ACTB), ARID1A/BAF250A, ARID1B/BAF250B, SMARCA2/BRM/BAF190B, SMARCA4/BRG1/BAF190A, ACTL6A/BAF53, ACTL6B/BAF53B, SMARCE1/BAF57, SMARCC1/BAF155, SMARCC2/BAF170, SMARCB1/SNF5/INI1, and one or more SMARCD1/BAF60A, SMARCD2/BAF60B, or SMARCD3/BAF60C. In muscle cells, the BAF complex also contains DPF3. Component of neural progenitors-specific chromatin remodeling complex (npBAF complex) composed of at least, ARID1A/BAF250A or ARID1B/BAF250B, SMARCD1/BAF60A, SMARCD3/BAF60C, SMARCA2/BRM/BAF190B, SMARCA4/BRG1/BAF190A, SMARCB1/BAF47, SMARCC1/BAF155, SMARCE1/BAF57, SMARCC2/BAF170, PHF10/BAF45A, ACTL6A/BAF53A and actin. Component of neuron-specific chromatin remodeling complex (nBAF complex) composed of at least, ARID1A/BAF250A or ARID1B/BAF250B, SMARCD1/BAF60A, SMARCD3/BAF60C, SMARCA2/BRM/BAF190B, SMARCA4/BRG1/BAF190A, SMARCB1/BAF47, SMARCC1/BAF155, SMARCE1/BAF57, SMARCC2/BAF170, DPF1/BAF45B, DPF3/BAF45C, ACTL6B/BAF53B and actin. May be a component of the SWI/SNF-B (PBAF) chromatin remodeling complex, at least composed of SMARCA4/BRG1, SMARCB1/BAF47/SNF5, ACTL6A/BAF53A or ACTL6B/BAF53B, SMARCE1/BAF57, SMARCD1/BAF60A, SMARCD2/BAF60B, perhaps SMARCD3/BAF60C, SMARCC1/BAF155, SMARCC2/BAF170, PBRM1/BAF180, ARID2/BAF200 and actin (ACTB). Interacts with BRDT (By similarity). Also binds to the SRC/p160 family of histone acetyltransferases (HATs) composed of NCOA1, NCOA2, and NCOA3. Interacts with RCOR1/CoREST, NR3C1 and ZMIM2/ZIMP7.
The HMG domain is essential for CD4 silencing and CD8 activation; mutation of this domain blocks thymus development.
研究领域
· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma. (View pathway)
文献引用
Application: WB Species: human Sample: T and NT tissues
Application: IHC Species: human Sample: 122 paired NT and human GC samples
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