DBI Antibody - #DF7307

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产品: DBI 抗体
货号: DF7307
描述: Rabbit polyclonal antibody to DBI
应用: WB IHC
反应: Human, Mouse, Rat
预测: Zebrafish, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
分子量: 10kDa; 10kD(Calculated).
蛋白号: P07108
RRID: AB_2839245

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
预测:
Zebrafish(92%), Bovine(%), Horse(%), Sheep(%), Rabbit(%), Chicken(%), Xenopus(%)
克隆:
Polyclonal
特异性:
DBI Antibody detects endogenous levels of total DBI.
RRID:
AB_2839245
引用格式: Affinity Biosciences Cat# DF7307, RRID:AB_2839245.
偶联:
Unconjugated. 130
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

ACBD 1; ACBD1; ACBP; ACBP_HUMAN; Acyl CoA binding domain containing 1; Acyl CoA binding protein; Acyl Coenzyme A binding domain containing 1; Acyl coenzyme A binding protein; Acyl-CoA-binding protein; CCK RP; CCKRP; Cholecystokinin releasing peptide trypsin sensitive; DBI; Diazepam-binding inhibitor; Endozepine; EP; GABA receptor modulator; MGC70414;

抗原和靶标

免疫原:
Uniprot:

P07108(ACBP_HUMAN) >>Visit HPA database.

基因/基因ID:
DBI(1622)
表达:
P07108 ACBP_HUMAN:

Isoform 1 is ubiquitous, with a moderate expression level. Isoform 2 is ubiquitous with high level in liver and adipose tissue. Isoform 3 is ubiquitous with strong expression in adipose tissue and heart.

描述:
This gene encodes diazepam binding inhibitor, a protein that is regulated by hormones and is involved in lipid metabolism and the displacement of beta-carbolines and benzodiazepines, which modulate signal transduction at type A gamma-aminobutyric acid receptors located in brain synapses. The protein is conserved from yeast to mammals, with the most highly conserved domain consisting of seven contiguous residues that constitute the hydrophobic binding site for medium- and long-chain acyl-Coenzyme A esters. Diazepam binding inhibitor is also known to mediate the feedback regulation of pancreatic secretion and the postprandial release of cholecystokinin, in addition to its role as a mediator in corticotropin-dependent adrenal steroidogenesis. Three pseudogenes located on chromosomes 6, 8 and 16 have been identified. Multiple transcript variants encoding different isoforms have been described for this gene.
序列:
MSQAEFEKAAEEVRHLKTKPSDEEMLFIYGHYKQATVGDINTERPGMLDFTGKAKWDAWNELKGTSKEDAMKAYINKVEELKKKYGI

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Bovine
92
Sheep
92
Zebrafish
92
Horse
83
Xenopus
83
Chicken
83
Rabbit
83
Pig
75
Dog
75
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P07108 作为底物

Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
K8 Acetylation
K17 Acetylation
K19 Acetylation
Y29 Phosphorylation
Y32 Phosphorylation
T42 Phosphorylation Q05655 (PRKCD)
T51 Phosphorylation
K53 Ubiquitination
K55 Acetylation
K55 Ubiquitination
K63 Ubiquitination
T65 Phosphorylation
S66 Phosphorylation
K77 Acetylation
K77 Ubiquitination
K82 Ubiquitination

研究背景

功能:

Binds medium- and long-chain acyl-CoA esters with very high affinity and may function as an intracellular carrier of acyl-CoA esters. It is also able to displace diazepam from the benzodiazepine (BZD) recognition site located on the GABA type A receptor. It is therefore possible that this protein also acts as a neuropeptide to modulate the action of the GABA receptor.

细胞定位:

Endoplasmic reticulum. Golgi apparatus.
Note: Golgi localization is dependent on ligand binding (PubMed:17953517).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Isoform 1 is ubiquitous, with a moderate expression level. Isoform 2 is ubiquitous with high level in liver and adipose tissue. Isoform 3 is ubiquitous with strong expression in adipose tissue and heart.

亚基结构:

Monomer.

蛋白家族:

Belongs to the ACBP family.

研究领域

· Organismal Systems > Endocrine system > PPAR signaling pathway.

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