GFRA1 Antibody - #DF7309

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产品: GFRA1 抗体
货号: DF7309
描述: Rabbit polyclonal antibody to GFRA1
应用: WB IHC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Sheep, Dog
分子量: 51kDa; 51kD(Calculated).
蛋白号: P56159
RRID: AB_2839247

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(80%), Bovine(90%), Sheep(90%), Dog(80%)
克隆:
Polyclonal
特异性:
GFRA1 Antibody detects endogenous levels of total GFRA1.
RRID:
AB_2839247
引用格式: Affinity Biosciences Cat# DF7309, RRID:AB_2839247.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

GDNF family receptor alpha 1; GDNF family receptor alpha-1; GDNF R; GDNF RA; GDNF receptor alpha; GDNF receptor alpha-1; GDNFR alpha 1; GDNFR alpha; GDNFR; GDNFR-alpha-1; GDNFRA; GDNFRalpha; GFR alpha 1; GFR alpha1; GFR-alpha-1; GFRA 1; Gfra1; GFRA1_HUMAN; GFRalpha1; Glial cell line derived neurotrophic factor receptor alpha; GPI linked anchor protein; MGC23045; PI linked cell surface accessory protein; RET 1L; RET ligand 1; RET1L; RETL 1; RETL1; TGF beta related neurotrophic factor receptor 1; TGF-beta-related neurotrophic factor receptor 1; TRNR 1; TRNR1;

抗原和靶标

免疫原:
Uniprot:

P56159(GFRA1_HUMAN) >>Visit HPA database.

基因/基因ID:
GFRA1(2674)
描述:
Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. The protein encoded by this gene is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol(GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. This gene is a candidate gene for Hirschsprung disease. Multiple alternatively spliced transcript variants have been described for this gene.
序列:
MFLATLYFALPLLDLLLSAEVSGGDRLDCVKASDQCLKEQSCSTKYRTLRQCVAGKETNFSLASGLEAKDECRSAMEALKQKSLYNCRCKRGMKKEKNCLRIYWSMYQSLQGNDLLEDSPYEPVNSRLSDIFRVVPFISDVFQQVEHIPKGNNCLDAAKACNLDDICKKYRSAYITPCTTSVSNDVCNRRKCHKALRQFFDKVPAKHSYGMLFCSCRDIACTERRRQTIVPVCSYEEREKPNCLNLQDSCKTNYICRSRLADFFTNCQPESRSVSSCLKENYADCLLAYSGLIGTVMTPNYIDSSSLSVAPWCDCSNSGNDLEECLKFLNFFKDNTCLKNAIQAFGNGSDVTVWQPAFPVQTTTATTTTALRVKNKPLGPAGSENEIPTHVLPPCANLQAQKLKSNVSGNTHLCISNGNYEKEGLGASSHITTKSMAAPPSCGLSPLLVLVVTALSTLLSLTETS

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Bovine
90
Sheep
90
Pig
80
Dog
80
Rabbit
70
Horse
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P56159 作为底物

Site PTM Type Enzyme
S183 Phosphorylation
T432 O-Glycosylation
T433 O-Glycosylation

研究背景

功能:

Receptor for GDNF. Mediates the GDNF-induced autophosphorylation and activation of the RET receptor (By similarity).

细胞定位:

Cell membrane>Lipid-anchor. Golgi apparatus>trans-Golgi network. Endosome. Endosome>Multivesicular body.
Note: Localizes mainly to the plasma membrane. In the presence of SORL1, shifts to vesicular structures, including trans-Golgi network, endosomes and multivesicular bodies.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

2 molecules of GDNFR-alpha are thought to form a complex with the disulfide-linked GDNF dimer and with 2 molecules of RET (By similarity). Interacts with RET. Interacts with SORL1, either alone or in complex with GDNF. Interaction between SORL1 and GFRA1 leads to GFRA1 internalization, but not degradation.

蛋白家族:

Belongs to the GDNFR family.

文献引用

1). Scorpion Venom Polypeptide Inhibits Pulmonary Epithelial-Mesenchymal Transition in Systemic Sclerosis-Interstitial Lung Disease Model Mice by Intervening TGF-β1/Smad Signaling Pathway. Evidence-based Complementary and Alternative Medicine, 2022 (PubMed: 35463079)

Application: WB    Species: Mouse    Sample: lung tissue

Figure 6 The expression levels of TGF-β1 (a), TβRI (b), TβRII (c), p-Smad2 (d), p-Smad3 (e), and Smad7 (f) protein and their protein band ((g), (h)) in the lung tissue of mice in each group. NC, normal control group; BLM, bleomycin-induced systemic sclerosis model group; PESV-L, low-dose PESV intervention group; PESV-M, medium-dose PESV intervention group; PESV-H, high-dose PESV intervention group; DXM, dexamethasone intervention group.

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