PA2G4 Antibody - #DF7311
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF7311, RRID:AB_2839249.
展开/折叠
38kDa; AA672939; Cell cycle protein p38 2G4 homolog; Cell cycle protein p38-2G4 homolog; ErbB-3 binding protein 1; ErbB3 binding protein 1; ErbB3-binding protein 1; ErbB3-binding protein Ebp1; hG4 1; hG4-1; IRES-specific cellular trans-acting factor 45 kDa; MGC81621; MGC94070; Mpp1; p38 2G4; Pa2g4; PA2G4_HUMAN; Plfap; Proliferation associated 2G4; Proliferation associated 2G4, 38-KD; Proliferation-associated 2G4, 38kDa; Proliferation-associated 2G4, a; Proliferation-associated protein 1; Proliferation-associated protein 2G4; Protein p38-2G4; si:dz150i12.2; wu:fb19b11; wu:ft56d05; zgc:86732;
抗原和靶标
Isoform 2 is undetectable whereas isoform 1 is strongly expressed in cancer cells (at protein level). Isoform 1 and isoform 2 are widely expressed, including heart, brain, lung, pancreas, skeletal muscle, kidney, placenta and liver.
- Q9UQ80 PA2G4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSGEDEQQEQTIAEDLVVTKYKMGGDIANRVLRSLVEASSSGVSVLSLCEKGDAMIMEETGKIFKKEKEMKKGIAFPTSISVNNCVCHFSPLKSDQDYILKEGDLVKIDLGVHVDGFIANVAHTFVVDVAQGTQVTGRKADVIKAAHLCAEAALRLVKPGNQNTQVTEAWNKVAHSFNCTPIEGMLSHQLKQHVIDGEKTIIQNPTDQQKKDHEKAEFEVHEVYAVDVLVSSGEGKAKDAGQRTTIYKRDPSKQYGLKMKTSRAFFSEVERRFDAMPFTLRAFEDEKKARMGVVECAKHELLQPFNVLYEKEGEFVAQFKFTVLLMPNGPMRITSGPFEPDLYKSEMEVQDAELKALLQSSASRKTQKKKKKKASKTAENATSGETLEENEAGD
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9UQ80 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| S2 | Phosphorylation | Uniprot | |
| T11 | Phosphorylation | Uniprot | |
| K20 | Ubiquitination | Uniprot | |
| K22 | Ubiquitination | Uniprot | |
| R30 | Methylation | Uniprot | |
| S34 | Phosphorylation | Uniprot | |
| S44 | Phosphorylation | Uniprot | |
| T60 | Phosphorylation | Uniprot | |
| K62 | Ubiquitination | Uniprot | |
| K72 | Ubiquitination | Uniprot | |
| S81 | Phosphorylation | Uniprot | |
| S90 | Phosphorylation | Uniprot | |
| K93 | Sumoylation | Uniprot | |
| K93 | Ubiquitination | Uniprot | |
| S94 | Phosphorylation | Uniprot | |
| Y98 | Phosphorylation | Uniprot | |
| K101 | Ubiquitination | Uniprot | |
| K144 | Ubiquitination | Uniprot | |
| K158 | Ubiquitination | Uniprot | |
| K172 | Methylation | Uniprot | |
| K172 | Ubiquitination | Uniprot | |
| K191 | Ubiquitination | Uniprot | |
| K199 | Methylation | Uniprot | |
| K199 | Ubiquitination | Uniprot | |
| T206 | Phosphorylation | Uniprot | |
| K210 | Ubiquitination | Uniprot | |
| K238 | Ubiquitination | Uniprot | |
| K248 | Ubiquitination | Uniprot | |
| K253 | Ubiquitination | Uniprot | |
| Y255 | Phosphorylation | Uniprot | |
| K258 | Ubiquitination | Uniprot | |
| K260 | Methylation | Uniprot | |
| T261 | Phosphorylation | Q13153 (PAK1) | Uniprot |
| S267 | Phosphorylation | Uniprot | |
| R271 | Methylation | Uniprot | |
| R281 | Methylation | Uniprot | |
| K287 | Acetylation | Uniprot | |
| K288 | Ubiquitination | Uniprot | |
| K298 | Acetylation | Uniprot | |
| K298 | Sumoylation | Uniprot | |
| K298 | Ubiquitination | Uniprot | |
| K311 | Ubiquitination | Uniprot | |
| S335 | Phosphorylation | Uniprot | |
| K344 | Sumoylation | Uniprot | |
| K344 | Ubiquitination | Uniprot | |
| S345 | Phosphorylation | Uniprot | |
| K355 | Ubiquitination | Uniprot | |
| S360 | Phosphorylation | P17252 (PRKCA) , Q05655 (PRKCD) | Uniprot |
| S361 | Phosphorylation | Uniprot | |
| S363 | Phosphorylation | P05129 (PRKCG) , P17252 (PRKCA) , P05771 (PRKCB) | Uniprot |
| T366 | Phosphorylation | P05771 (PRKCB) , P17252 (PRKCA) , P05129 (PRKCG) | Uniprot |
| K373 | Ubiquitination | Uniprot | |
| S375 | Phosphorylation | Uniprot | |
| K376 | Ubiquitination | Uniprot | |
| T377 | Phosphorylation | Uniprot | |
| T382 | Phosphorylation | Uniprot | |
| S383 | Phosphorylation | Uniprot | |
| T386 | Phosphorylation | Uniprot |
研究背景
May play a role in a ERBB3-regulated signal transduction pathway. Seems be involved in growth regulation. Acts a corepressor of the androgen receptor (AR) and is regulated by the ERBB3 ligand neuregulin-1/heregulin (HRG). Inhibits transcription of some E2F1-regulated promoters, probably by recruiting histone acetylase (HAT) activity. Binds RNA. Associates with 28S, 18S and 5.8S mature rRNAs, several rRNA precursors and probably U3 small nucleolar RNA. May be involved in regulation of intermediate and late steps of rRNA processing. May be involved in ribosome assembly. Mediates cap-independent translation of specific viral IRESs (internal ribosomal entry site) (By similarity). Regulates cell proliferation, differentiation, and survival. Isoform 1 suppresses apoptosis whereas isoform 2 promotes cell differentiation (By similarity).
Phosphorylated on serine and threonine residues. Phosphorylation is enhanced by HRG treatment. Basal phosphorylation is PKC-dependent and HRG-induced phosphorylation is predominantly PKC-independent. Phosphorylation at Ser-361 by PKC/PRKCD regulates its nucleolar localization.
In cancer cells, isoform 2 is polyubiquitinated leading to its proteasomal degradation and phosphorylation by PKC/PRKCD enhances polyubiquitination.
Cytoplasm. Nucleus>Nucleolus.
Note: Translocates to the nucleus upon treatment with HRG. Phosphorylation at Ser-361 by PKC/PRKCD regulates its nucleolar localization.
Cytoplasm.
Isoform 2 is undetectable whereas isoform 1 is strongly expressed in cancer cells (at protein level). Isoform 1 and isoform 2 are widely expressed, including heart, brain, lung, pancreas, skeletal muscle, kidney, placenta and liver.
Isoform 2 interacts with the cytoplasmic domain of non-phosphorylated ERBB3; the interaction requires PKC activity. Interacts with AR. Treatment with HRG leads to dissociation from ERBB3 and increases association with AR. Interacts with NCL/nucleolin. Component of a ribonucleoprotein complex containing at least PA2G4, NCL, TOP1, PABPC2, RPLP0, acetylated histone H1 (HIST1H1A or H1F1), histone H1 2/4, RPL4, RPL8, RPL15, RPL18, RPL18A, RPL21, RPL11, RPL12, RPL28, RPL27, RPLP2 and RPL24. Interacts with HDAC2. Interacts with RB1; the interaction is enhanced upon PA2G4 dephosphorylation. Interacts with AKT1 (By similarity). Isoform 1 and isoform 2 interact with RNF20. Isoform 2 interacts with HUWE1. Interacts with DNAJC21.
Belongs to the peptidase M24 family.
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