产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF7315, RRID:AB_2839253.
展开/折叠
Fug 1; Fug1; GTPase-activating protein, RAN, 1; KIAA1835; MGC20266; OTTHUMP00000028918; OTTHUMP00000198755; OTTHUMP00000198756; OTTHUMP00000198757; OTTHUMP00000198758; RAGP1_HUMAN; Ran 1; RAN GTPase activating protein 1; Ran GTPase-activating protein 1; Ran1; RANGAP 1; RANGAP; RanGAP1; SD; Segregation distorter homolog; Segregation distortion;
抗原和靶标
- P46060 RAGP1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MASEDIAKLAETLAKTQVAGGQLSFKGKSLKLNTAEDAKDVIKEIEDFDSLEALRLEGNTVGVEAARVIAKALEKKSELKRCHWSDMFTGRLRTEIPPALISLGEGLITAGAQLVELDLSDNAFGPDGVQGFEALLKSSACFTLQELKLNNCGMGIGGGKILAAALTECHRKSSAQGKPLALKVFVAGRNRLENDGATALAEAFRVIGTLEEVHMPQNGINHPGITALAQAFAVNPLLRVINLNDNTFTEKGAVAMAETLKTLRQVEVINFGDCLVRSKGAVAIADAIRGGLPKLKELNLSFCEIKRDAALAVAEAMADKAELEKLDLNGNTLGEEGCEQLQEVLEGFNMAKVLASLSDDEDEEEEEEGEEEEEEAEEEEEEDEEEEEEEEEEEEEEPQQRGQGEKSATPSRKILDPNTGEPAPVLSSPPPADVSTFLAFPSPEKLLRLGPKSSVLIAQQTDTSDPEKVVSAFLKVSSVFKDEATVRMAVQDAVDALMQKAFNSSSFNSNTFLTRLLVHMGLLKSEDKVKAIANLYGPLMALNHMVQQDYFPKALAPLLLAFVTKPNSALESCSFARHSLLQTLYKV
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P46060 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| S3 | Phosphorylation | Uniprot | |
| K8 | Sumoylation | Uniprot | |
| K8 | Ubiquitination | Uniprot | |
| K15 | Ubiquitination | Uniprot | |
| S24 | Phosphorylation | Uniprot | |
| K26 | Acetylation | Uniprot | |
| K26 | Sumoylation | Uniprot | |
| K26 | Ubiquitination | Uniprot | |
| K28 | Sumoylation | Uniprot | |
| S29 | Phosphorylation | Uniprot | |
| K31 | Ubiquitination | Uniprot | |
| K39 | Ubiquitination | Uniprot | |
| K43 | Ubiquitination | Uniprot | |
| K71 | Ubiquitination | Uniprot | |
| R81 | Methylation | Uniprot | |
| K148 | Ubiquitination | Uniprot | |
| C169 | S-Nitrosylation | Uniprot | |
| K172 | Ubiquitination | Uniprot | |
| K178 | Ubiquitination | Uniprot | |
| K183 | Ubiquitination | Uniprot | |
| T247 | Phosphorylation | Uniprot | |
| K251 | Ubiquitination | Uniprot | |
| K261 | Ubiquitination | Uniprot | |
| S278 | Phosphorylation | Uniprot | |
| K279 | Ubiquitination | Uniprot | |
| R289 | Methylation | Uniprot | |
| K296 | Ubiquitination | Uniprot | |
| S301 | Phosphorylation | Uniprot | |
| K306 | Ubiquitination | Uniprot | |
| K320 | Ubiquitination | Uniprot | |
| S358 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| K406 | Methylation | Uniprot | |
| T409 | Phosphorylation | P06493 (CDK1) | Uniprot |
| K413 | Acetylation | Uniprot | |
| T419 | Phosphorylation | Uniprot | |
| S427 | Phosphorylation | Uniprot | |
| S428 | Phosphorylation | P06493 (CDK1) | Uniprot |
| S435 | Phosphorylation | Uniprot | |
| T436 | Phosphorylation | Uniprot | |
| S442 | Phosphorylation | P06493 (CDK1) | Uniprot |
| K452 | Sumoylation | Uniprot | |
| K452 | Ubiquitination | Uniprot | |
| S453 | Phosphorylation | Uniprot | |
| T461 | Phosphorylation | Uniprot | |
| T463 | Phosphorylation | Uniprot | |
| S464 | Phosphorylation | Uniprot | |
| K468 | Ubiquitination | Uniprot | |
| S478 | Phosphorylation | Uniprot | |
| K481 | Ubiquitination | Uniprot | |
| T485 | Phosphorylation | Uniprot | |
| S505 | Phosphorylation | Uniprot | |
| K524 | Acetylation | Uniprot | |
| K524 | Sumoylation | Uniprot | |
| K524 | Ubiquitination | Uniprot | |
| K528 | Sumoylation | Uniprot | |
| K530 | Sumoylation | Uniprot | |
| Y585 | Phosphorylation | Uniprot |
研究背景
GTPase activator for RAN. Converts cytoplasmic GTP-bound RAN to GDP-bound RAN, which is essential for RAN-mediated nuclear import and export. Mediates dissociation of cargo from nuclear export complexes containing XPO1, RAN and RANBP2 after nuclear export.
Phosphorylation occurs before nuclear envelope breakdown and continues throughout mitosis. Phosphorylated by the M-phase kinase cyclin B/Cdk1, in vitro. Differential timimg of dephosphorylation occurs during phases of mitosis. The phosphorylated form remains associated with RANBP2/NUP358 and the SUMO E2-conjugating enzyme, UBE2I, on nuclear pore complex (NPC) diassembly and during mitosis.
Sumoylated. Sumoylation is necessary for targeting to the nuclear envelope (NE), and for association with mitotic spindles and kinetochores during mitosis. Also required for interaction with RANBP2 and is mediated by UBE2I.
Cytoplasm. Nucleus>Nucleoplasm. Nucleus envelope. Chromosome>Centromere>Kinetochore. Cytoplasm>Cytoskeleton>Spindle.
Note: Cytoplasmic during interphase. Detected at the nuclear envelope during interphase (PubMed:11854305, PubMed:15037602). Targeted to the nuclear pores after sumoylation (PubMed:11854305). During mitosis, associates with mitotic spindles, but is essentially not detected at the spindle poles (PubMed:11854305, PubMed:15037602). Association with kinetochores appears soon after nuclear envelope breakdown and persists until late anaphase (PubMed:11854305). Mitotic location also requires sumoylation (PubMed:11854305).
Highly expressed in brain, thymus and testis.
Homodimer. Interacts with RAN. Forms a complex with RANBP2/NUP358, NXF1 and NXT1. Forms a tight complex in association with RANBP2/NUP358 and UBE2I/UBC9, the ubiquitin-conjugating enzyme E2. Interacts with UBE2I; the interaction conjugates SUMO1 to RANGAP1, and subsequently stabilizes interactions of sumoylated RANGAP1 with RANBP2/NUP358. The complex composed of RANBP2, SUMO1, RANGAP1 and UBE2I associates with nuclear pore complexes. Identified in a complex composed of RAN, RANBP2, sumoylated RANGAP1, UBE2I and XPO1. Identified in a complex composed of RAN, RANGAP1 and RANBP1. Interacts with TRAF6. Interacts with SUMO1 and SENP1. Interacts (when sumoylated) with MYCBP2; interaction inhibits MYCBP2 E3 ubiquitin-protein ligase activity and promotes MYCBP2 translocation to the nucleus.
Belongs to the RNA1 family.
研究领域
· Genetic Information Processing > Translation > RNA transport.
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