(2)
(3)
| 产品: | DLD 抗体 |
| 货号: | DF7322 |
| 描述: | Rabbit polyclonal antibody to DLD |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 54kDa; 54kD(Calculated). |
| 蛋白号: | P09622 |
| RRID: | AB_2839260 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF7322, RRID:AB_2839260.
展开/折叠
Dehydrogenase complex, E3 component; Diaphorase; Dihydrolipoamide dehydrogenase; Dihydrolipoyl dehydrogenase; Dihydrolipoyl dehydrogenase mitochondrial; dld; DLDD; DLDH; DLDH_HUMAN; E3; E3 branched chain aplha-keto acid; E3 component of pyruvate dehydrogenase; E3 component of pyruvate dehydrogenase complex 2 oxo glutarate complex branched chain keto acid dehydrogenase complex; GCSL; Glycine cleavage system L protein; Glycine cleavage system protein L; LAD; lipoamide dehydrogenase; Lipoamide reductase; Lipoyl dehydrogenase; mitochondrial; OTTHUMP00000206744; OTTHUMP00000206746; OTTHUMP00000206748; OTTHUMP00000206749; PHE 3; PHE3; Pyruvate dehydrogenase component E3;
抗原和靶标
- P09622 DLDH_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MQSWSRVYCSLAKRGHFNRISHGLQGLSAVPLRTYADQPIDADVTVIGSGPGGYVAAIKAAQLGFKTVCIEKNETLGGTCLNVGCIPSKALLNNSHYYHMAHGKDFASRGIEMSEVRLNLDKMMEQKSTAVKALTGGIAHLFKQNKVVHVNGYGKITGKNQVTATKADGGTQVIDTKNILIATGSEVTPFPGITIDEDTIVSSTGALSLKKVPEKMVVIGAGVIGVELGSVWQRLGADVTAVEFLGHVGGVGIDMEISKNFQRILQKQGFKFKLNTKVTGATKKSDGKIDVSIEAASGGKAEVITCDVLLVCIGRRPFTKNLGLEELGIELDPRGRIPVNTRFQTKIPNIYAIGDVVAGPMLAHKAEDEGIICVEGMAGGAVHIDYNCVPSVIYTHPEVAWVGKSEEQLKEEGIEYKVGKFPFAANSRAKTNADTDGMVKILGQKSTDRVLGAHILGPGAGEMVNEAALALEYGASCEDIARVCHAHPTLSEAFREANLAASFGKSINF
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P09622 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K66 | Acetylation | Uniprot | |
| K66 | Methylation | Uniprot | |
| K72 | Acetylation | Uniprot | |
| K104 | Acetylation | Uniprot | |
| K104 | Ubiquitination | Uniprot | |
| R109 | Methylation | Uniprot | |
| K122 | Acetylation | Uniprot | |
| K127 | Ubiquitination | Uniprot | |
| K132 | Ubiquitination | Uniprot | |
| T135 | Phosphorylation | Uniprot | |
| K143 | Acetylation | Uniprot | |
| K143 | Methylation | Uniprot | |
| K143 | Ubiquitination | Uniprot | |
| K146 | Acetylation | Uniprot | |
| Y153 | Phosphorylation | Uniprot | |
| K155 | Acetylation | Uniprot | |
| K159 | Acetylation | Uniprot | |
| K159 | Ubiquitination | Uniprot | |
| K166 | Acetylation | Uniprot | |
| K166 | Ubiquitination | Uniprot | |
| S230 | Phosphorylation | Uniprot | |
| K267 | Acetylation | Uniprot | |
| K267 | Ubiquitination | Uniprot | |
| K271 | Acetylation | Uniprot | |
| K273 | Acetylation | Uniprot | |
| T276 | Phosphorylation | Uniprot | |
| K277 | Acetylation | Uniprot | |
| K277 | Ubiquitination | Uniprot | |
| S297 | Phosphorylation | Uniprot | |
| K320 | Acetylation | Uniprot | |
| K320 | Ubiquitination | Uniprot | |
| K346 | Acetylation | Uniprot | |
| K346 | Ubiquitination | Uniprot | |
| K410 | Acetylation | Uniprot | |
| K410 | Ubiquitination | Uniprot | |
| K417 | Acetylation | Uniprot | |
| K417 | Ubiquitination | Uniprot | |
| K420 | Acetylation | Uniprot | |
| K420 | Ubiquitination | Uniprot | |
| K430 | Ubiquitination | Uniprot | |
| T435 | Phosphorylation | Uniprot | |
| K440 | Ubiquitination | Uniprot | |
| K445 | Ubiquitination | Uniprot | |
| S502 | Phosphorylation | Uniprot | |
| K505 | Methylation | Uniprot |
研究背景
Lipoamide dehydrogenase is a component of the glycine cleavage system as well as an E3 component of three alpha-ketoacid dehydrogenase complexes (pyruvate-, alpha-ketoglutarate-, and branched-chain amino acid-dehydrogenase complex). The 2-oxoglutarate dehydrogenase complex is mainly active in the mitochondrion. A fraction of the 2-oxoglutarate dehydrogenase complex also localizes in the nucleus and is required for lysine succinylation of histones: associates with KAT2A on chromatin and provides succinyl-CoA to histone succinyltransferase KAT2A. In monomeric form may have additional moonlighting function as serine protease. Involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction (By similarity).
Tyrosine phosphorylated.
Mitochondrion matrix. Nucleus. Cell projection>Cilium>Flagellum. Cytoplasmic vesicle>Secretory vesicle>Acrosome.
Note: Mainly localizes in the mitochondrion. A small fraction localizes to the nucleus, where the 2-oxoglutarate dehydrogenase complex is required for histone succinylation.
Homodimer. Part of the multimeric pyruvate dehydrogenase complex that contains multiple copies of pyruvate dehydrogenase (subunits PDHA (PDHA1 or PDHA2) and PDHB, E1), dihydrolipoamide acetyltransferase (DLAT, E2) and lipoamide dehydrogenase (DLD, E3). These subunits are bound to an inner core composed of about 48 DLAT and 12 PDHX molecules (by non covalent bonds). The 2-oxoglutarate dehydrogenase complex is composed of OGDH (2-oxoglutarate dehydrogenase; E1), DLST (dihydrolipoamide succinyltransferase; E2) and DLD (dihydrolipoamide dehydrogenase; E3). It contains multiple copies of the three enzymatic components (E1, E2 and E3). In the nucleus, the 2-oxoglutarate dehydrogenase complex associates with KAT2A. Interacts with PDHX.
Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.
研究领域
· Metabolism > Carbohydrate metabolism > Glycolysis / Gluconeogenesis.
· Metabolism > Carbohydrate metabolism > Citrate cycle (TCA cycle).
· Metabolism > Amino acid metabolism > Glycine, serine and threonine metabolism.
· Metabolism > Amino acid metabolism > Valine, leucine and isoleucine degradation.
· Metabolism > Carbohydrate metabolism > Pyruvate metabolism.
· Metabolism > Carbohydrate metabolism > Glyoxylate and dicarboxylate metabolism.
· Metabolism > Carbohydrate metabolism > Propanoate metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
· Metabolism > Global and overview maps > Carbon metabolism.
文献引用
Application: IHC Species: Rat Sample:
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