产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF7329, RRID:AB_2839267.
展开/折叠
from Entrez Gene ;; HEC; Highly expressed in cancer; Highly expressed in cancer protein; Highly expressed in cancer rich in leucine heptad repeats; HsHec1; hsNDC80; Kinetochore associated 2; Kinetochore associated protein 2; Kinetochore protein Hec1; Kinetochore protein NDC80 homolog; Kinetochore-associated protein 2; KNTC2; ndc80; NDC80 homolog kinetochore complex component; NDC80 kinetochore complex component homolog; NDC80, S. cerevisiae, homolog of; NDC80_HUMAN; Retinoblastoma associated protein HEC; Retinoblastoma-associated protein HEC; TID3;
抗原和靶标
- O14777 NDC80_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MKRSSVSSGGAGRLSMQELRSQDVNKQGLYTPQTKEKPTFGKLSINKPTSERKVSLFGKRTSGHGSRNSQLGIFSSSEKIKDPRPLNDKAFIQQCIRQLCEFLTENGYAHNVSMKSLQAPSVKDFLKIFTFLYGFLCPSYELPDTKFEEEVPRIFKDLGYPFALSKSSMYTVGAPHTWPHIVAALVWLIDCIKIHTAMKESSPLFDDGQPWGEETEDGIMHNKLFLDYTIKCYESFMSGADSFDEMNAELQSKLKDLFNVDAFKLESLEAKNRALNEQIARLEQEREKEPNRLESLRKLKASLQGDVQKYQAYMSNLESHSAILDQKLNGLNEEIARVELECETIKQENTRLQNIIDNQKYSVADIERINHERNELQQTINKLTKDLEAEQQKLWNEELKYARGKEAIETQLAEYHKLARKLKLIPKGAENSKGYDFEIKFNPEAGANCLVKYRAQVYVPLKELLNETEEEINKALNKKMGLEDTLEQLNAMITESKRSVRTLKEEVQKLDDLYQQKIKEAEEEDEKCASELESLEKHKHLLESTVNQGLSEAMNELDAVQREYQLVVQTTTEERRKVGNNLQRLLEMVATHVGSVEKHLEEQIAKVDREYEECMSEDLSENIKEIRDKYEKKATLIKSSEE
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O14777 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S5 | Phosphorylation | Q96GD4 (AURKB) | Uniprot |
| S15 | Phosphorylation | Q96GD4 (AURKB) | Uniprot |
| R20 | Methylation | Uniprot | |
| K26 | Ubiquitination | Uniprot | |
| Y30 | Phosphorylation | Uniprot | |
| T31 | Phosphorylation | Uniprot | |
| T34 | Phosphorylation | Uniprot | |
| K37 | Ubiquitination | Uniprot | |
| T39 | Phosphorylation | Uniprot | |
| K42 | Ubiquitination | Uniprot | |
| S44 | Phosphorylation | Q96GD4 (AURKB) | Uniprot |
| K47 | Ubiquitination | Uniprot | |
| T49 | Phosphorylation | Q96GD4 (AURKB) | Uniprot |
| S50 | Phosphorylation | Uniprot | |
| K53 | Acetylation | Uniprot | |
| S55 | Phosphorylation | Q96GD4 (AURKB) , O14965 (AURKA) | Uniprot |
| K59 | Acetylation | Uniprot | |
| K59 | Methylation | Uniprot | |
| K59 | Ubiquitination | Uniprot | |
| T61 | Phosphorylation | Uniprot | |
| S62 | Phosphorylation | Uniprot | |
| S66 | Phosphorylation | Uniprot | |
| S69 | Phosphorylation | Q96GD4 (AURKB) | Uniprot |
| S76 | Phosphorylation | Uniprot | |
| S77 | Phosphorylation | Uniprot | |
| K81 | Ubiquitination | Uniprot | |
| K89 | Ubiquitination | Uniprot | |
| K123 | Ubiquitination | Uniprot | |
| R153 | Methylation | Uniprot | |
| K156 | Ubiquitination | Uniprot | |
| S165 | Phosphorylation | P51955 (NEK2) , Q96PY6 (NEK1) | Uniprot |
| S202 | Phosphorylation | Uniprot | |
| K253 | Ubiquitination | Uniprot | |
| K255 | Ubiquitination | Uniprot | |
| K264 | Ubiquitination | Uniprot | |
| K271 | Ubiquitination | Uniprot | |
| K300 | Ubiquitination | Uniprot | |
| K309 | Ubiquitination | Uniprot | |
| K327 | Ubiquitination | Uniprot | |
| K346 | Ubiquitination | Uniprot | |
| K360 | Ubiquitination | Uniprot | |
| Y361 | Phosphorylation | Uniprot | |
| K382 | Ubiquitination | Uniprot | |
| K393 | Ubiquitination | Uniprot | |
| K400 | Ubiquitination | Uniprot | |
| K405 | Ubiquitination | Uniprot | |
| K417 | Ubiquitination | Uniprot | |
| K433 | Ubiquitination | Uniprot | |
| K452 | Ubiquitination | Uniprot | |
| Y458 | Phosphorylation | Uniprot | |
| K462 | Ubiquitination | Uniprot | |
| K474 | Ubiquitination | Uniprot | |
| T502 | Phosphorylation | Uniprot | |
| K504 | Ubiquitination | Uniprot | |
| K509 | Ubiquitination | Uniprot | |
| K517 | Ubiquitination | Uniprot | |
| K527 | Acetylation | Uniprot | |
| S530 | Phosphorylation | Uniprot | |
| T591 | Phosphorylation | Uniprot | |
| S595 | Phosphorylation | Uniprot | |
| K606 | Ubiquitination | Uniprot | |
| S616 | Phosphorylation | Uniprot |
研究背景
Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules. Plays a role in chromosome congression and is essential for the end-on attachment of the kinetochores to spindle microtubules.
Phosphorylation begins in S phase of the cell cycle and peaks in mitosis. Phosphorylated by NEK2. May also be phosphorylated by AURKA and AURKB.
Nucleus. Chromosome>Centromere>Kinetochore.
Note: Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore.
Component of the NDC80 complex, which consists of NDC80/HEC1, CDCA1, SPBC24 and SPBC25. The NDC80 complex is formed by two subcomplexes composed of NDC80/HEC1-CDCA1 and SPBC24-SPBC25. Each subcomplex is formed by parallel interactions through the coiled-coil domains of individual subunits. Formation of a tetrameric complex is mediated by interactions between the C-terminal regions of both subunits of the NDC80/HEC1-CDCA1 subcomplex and the N-terminal regions of both subunits of the SPBC24-SPBC25 complex. The tetrameric NDC80 complex has an elongated rod-like structure with globular domains at either end. Interacts with isoform 1 of NEK2 and ZWINT specifically during mitosis. Interacts with CENPH and MIS12. May interact with AURKB, PSMC2, PSMC5 and SMC1A. May interact with RB1 during G2 phase and mitosis. Interacts with CKAP5. Interacts with CDT1; leading to kinetochore localization of CDT1.
Belongs to the NDC80/HEC1 family.
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