| 产品: | NPC2 抗体 |
| 货号: | DF7331 |
| 描述: | Rabbit polyclonal antibody to NPC2 |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Horse, Dog |
| 分子量: | 17kDa; 17kD(Calculated). |
| 蛋白号: | P61916 |
| RRID: | AB_2839269 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF7331, RRID:AB_2839269.
展开/折叠
EDDM1; Epididymal protein 1; Epididymal secretory protein; Epididymal secretory protein E1; hE1; Human epididymis-specific protein 1; Niemann-Pick disease type C2; Niemann-Pick disease type C2 protein; NPC intracellular cholesterol transporter 2; NPC2; NPC2_HUMAN; Tissue specific secretory protein;
抗原和靶标
Detected in gallbladder bile (PubMed:21315718). Detected in fibroblasts, kidney, liver, spleen, small intestine, placenta and testis (at protein level) (PubMed:11125141). Epididymis.
- P61916 NPC2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MRFLAATFLLLALSTAAQAEPVQFKDCGSVDGVIKEVNVSPCPTQPCQLSKGQSYSVNVTFTSNIQSKSSKAVVHGILMGVPVPFPIPEPDGCKSGINCPIQKDKTYSYLNKLPVKSEYPSIKLVVEWQLQDDKNQSLFCWEIPVQIVSHL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P61916 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S14 | Phosphorylation | Uniprot | |
| S29 | Phosphorylation | Uniprot | |
| N58 | N-Glycosylation | Uniprot | |
| K105 | Ubiquitination | Uniprot | |
| T106 | Phosphorylation | Uniprot | |
| Y107 | Phosphorylation | Uniprot | |
| S108 | Phosphorylation | Uniprot | |
| K112 | Ubiquitination | Uniprot | |
| Y119 | Phosphorylation | Uniprot | |
| N135 | N-Glycosylation | Uniprot |
研究背景
Intracellular cholesterol transporter which acts in concert with NPC1 and plays an important role in the egress of cholesterol from the lysosomal compartment. Unesterified cholesterol that has been released from LDLs in the lumen of the late endosomes/lysosomes is transferred by NPC2 to the cholesterol-binding pocket in the N-terminal domain of NPC1. May bind and mobilize cholesterol that is associated with membranes. NPC2 binds cholesterol with a 1:1 stoichiometry. Can bind a variety of sterols, including lathosterol, desmosterol and the plant sterols stigmasterol and beta-sitosterol. The secreted form of NCP2 regulates biliary cholesterol secretion via stimulation of ABCG5/ABCG8-mediated cholesterol transport (By similarity).
Secreted. Endoplasmic reticulum. Lysosome.
Note: Interaction with cell-surface M6PR mediates endocytosis and targeting to lysosomes.
Detected in gallbladder bile. Detected in fibroblasts, kidney, liver, spleen, small intestine, placenta and testis (at protein level). Epididymis.
Interacts with NPC1 (via the second lumenal domain) in a cholestrol-dependent manner. Interacts with NUS1/NgBR, the interaction stabilizes NCP2 and regulates cholesterol trafficking. Interacts with DHDDS. Interacts with NEDD4L (via C2 domain). Interacts with NPC1L1.
Binds cholesterol in a hydrophobic pocket; there are no hydrogen bonds between the sterol and the protein.
Belongs to the NPC2 family.
研究领域
· Cellular Processes > Transport and catabolism > Lysosome. (View pathway)
· Organismal Systems > Digestive system > Cholesterol metabolism.
限制条款
产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。
产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。
Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。产品仅供科学研究使用。不用于诊断和治疗。
产品未经授权不得转售。
Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.