产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF7340, RRID:AB_2839278.
展开/折叠
AP 4 A synthetase; Charcot Marie Tooth neuropathy 2D; Charcot Marie Tooth neuropathy neuronal type D; CMT2D; Diadenosine tetraphosphate synthetase; DSMAV; EC 6.1.1.14; Glycine tRNA ligase; Glycyl tRNA synthetase; GlyRS; HMN5; SMAD1;
抗原和靶标
- P41250 GARS_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPSPRPVLLRGARAALLLLLPPRLLARPSLLLRRSLSAASCPPISLPAAASRSSMDGAGAEEVLAPLRLAVRQQGDLVRKLKEDKAPQVDVDKAVAELKARKRVLEAKELALQPKDDIVDRAKMEDTLKRRFFYDQAFAIYGGVSGLYDFGPVGCALKNNIIQTWRQHFIQEEQILEIDCTMLTPEPVLKTSGHVDKFADFMVKDVKNGECFRADHLLKAHLQKLMSDKKCSVEKKSEMESVLAQLDNYGQQELADLFVNYNVKSPITGNDLSPPVSFNLMFKTFIGPGGNMPGYLRPETAQGIFLNFKRLLEFNQGKLPFAAAQIGNSFRNEISPRSGLIRVREFTMAEIEHFVDPSEKDHPKFQNVADLHLYLYSAKAQVSGQSARKMRLGDAVEQGVINNTVLGYFIGRIYLYLTKVGISPDKLRFRQHMENEMAHYACDCWDAESKTSYGWIEIVGCADRSCYDLSCHARATKVPLVAEKPLKEPKTVNVVQFEPSKGAIGKAYKKDAKLVMEYLAICDECYITEMEMLLNEKGEFTIETEGKTFQLTKDMINVKRFQKTLYVEEVVPNVIEPSFGLGRIMYTVFEHTFHVREGDEQRTFFSFPAVVAPFKCSVLPLSQNQEFMPFVKELSEALTRHGVSHKVDDSSGSIGRRYARTDEIGVAFGVTIDFDTVNKTPHTATLRDRDSMRQIRAEISELPSIVQDLANGNITWADVEARYPLFEGQETGKKETIEE
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P41250 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S29 | Phosphorylation | Uniprot | |
| S35 | Phosphorylation | Uniprot | |
| S37 | Phosphorylation | Uniprot | |
| S53 | Phosphorylation | Uniprot | |
| S54 | Phosphorylation | Uniprot | |
| K80 | Ubiquitination | Uniprot | |
| K82 | Sumoylation | Uniprot | |
| K82 | Ubiquitination | Uniprot | |
| K85 | Ubiquitination | Uniprot | |
| K93 | Acetylation | Uniprot | |
| K93 | Ubiquitination | Uniprot | |
| K99 | Acetylation | Uniprot | |
| K99 | Ubiquitination | Uniprot | |
| K108 | Acetylation | Uniprot | |
| K108 | Ubiquitination | Uniprot | |
| K115 | Acetylation | Uniprot | |
| K115 | Ubiquitination | Uniprot | |
| K123 | Ubiquitination | Uniprot | |
| K129 | Ubiquitination | Uniprot | |
| Y141 | Phosphorylation | Uniprot | |
| S145 | Phosphorylation | Uniprot | |
| Y148 | Phosphorylation | Uniprot | |
| T191 | Phosphorylation | Uniprot | |
| S192 | Phosphorylation | Uniprot | |
| K197 | Ubiquitination | Uniprot | |
| K204 | Acetylation | Uniprot | |
| K204 | Ubiquitination | Uniprot | |
| K207 | Ubiquitination | Uniprot | |
| K219 | Acetylation | Uniprot | |
| K219 | Ubiquitination | Uniprot | |
| K224 | Ubiquitination | Uniprot | |
| K309 | Acetylation | Uniprot | |
| K309 | Ubiquitination | Uniprot | |
| K318 | Ubiquitination | Uniprot | |
| S335 | Phosphorylation | Uniprot | |
| S377 | Phosphorylation | Uniprot | |
| K379 | Ubiquitination | Uniprot | |
| Y414 | Phosphorylation | Uniprot | |
| K419 | Ubiquitination | Uniprot | |
| S423 | Phosphorylation | Uniprot | |
| K426 | Ubiquitination | Uniprot | |
| Y453 | Phosphorylation | Uniprot | |
| C466 | S-Nitrosylation | Uniprot | |
| Y467 | Phosphorylation | Uniprot | |
| C471 | S-Nitrosylation | Uniprot | |
| K484 | Acetylation | Uniprot | |
| K484 | Ubiquitination | Uniprot | |
| K487 | Ubiquitination | Uniprot | |
| S500 | Phosphorylation | Uniprot | |
| K501 | Acetylation | Uniprot | |
| K501 | Ubiquitination | Uniprot | |
| K506 | Ubiquitination | Uniprot | |
| Y518 | Phosphorylation | Uniprot | |
| K547 | Ubiquitination | Uniprot | |
| T548 | Phosphorylation | Uniprot | |
| T552 | Phosphorylation | Uniprot | |
| K553 | Ubiquitination | Uniprot | |
| K559 | Acetylation | Uniprot | |
| K559 | Ubiquitination | Uniprot | |
| K563 | Ubiquitination | Uniprot | |
| R596 | Methylation | Uniprot | |
| S606 | Phosphorylation | Uniprot | |
| K646 | Ubiquitination | Uniprot | |
| S650 | Phosphorylation | Uniprot | |
| S653 | Phosphorylation | Uniprot | |
| R687 | Methylation | Uniprot | |
| K733 | Ubiquitination | Uniprot | |
| K734 | Ubiquitination | Uniprot | |
| T736 | Phosphorylation | Uniprot |
研究背景
Catalyzes the ATP-dependent ligation of glycine to the 3'-end of its cognate tRNA, via the formation of an aminoacyl-adenylate intermediate (Gly-AMP). Also produces diadenosine tetraphosphate (Ap4A), a universal pleiotropic signaling molecule needed for cell regulation pathways, by direct condensation of 2 ATPs. Thereby, may play a special role in Ap4A homeostasis.
Cytoplasm. Mitochondrion. Cell projection>Axon. Secreted. Secreted>Extracellular exosome.
Note: In transfected COS7 cells, not detected in mitochondria, nor in Golgi apparatus (PubMed:17035524). Secreted by motor neuron, possibly through the exosome pathway (By similarity).
Widely expressed, including in brain and spinal cord.
Homodimer.
Belongs to the class-II aminoacyl-tRNA synthetase family.
研究领域
· Genetic Information Processing > Translation > Aminoacyl-tRNA biosynthesis.
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