RFC4 Antibody - #DF7367

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产品: RFC4 抗体
货号: DF7367
描述: Rabbit polyclonal antibody to RFC4
应用: WB IHC
反应: Human, Mouse, Rat, Monkey
预测: Pig, Bovine, Horse, Rabbit, Dog, Xenopus
分子量: 39kDa; 40kD(Calculated).
蛋白号: P35249
RRID: AB_2839305

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat,Monkey
预测:
Pig(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Xenopus(91%)
克隆:
Polyclonal
特异性:
RFC4 Antibody detects endogenous levels of total RFC4.
RRID:
AB_2839305
引用格式: Affinity Biosciences Cat# DF7367, RRID:AB_2839305.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

A1 37; A1 37 kDa subunit; Activator 1 37; Activator 1 37 kDa subunit; Activator 1 subunit 4; Replication factor C 37; Replication factor C 37 kDa subunit; Replication factor C subunit 4; Replication factor C4; RF-C 37 kDa subunit; RFC 37; RFC37; RFC4 replication factor C (activator 1)4 37kDa; RfC4; RFC4_HUMAN;

抗原和靶标

免疫原:
Uniprot:

P35249(RFC4_HUMAN) >>Visit HPA database.

基因/基因ID:
RFC4(5984)
描述:
The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported.
序列:
MQAFLKGTSISTKPPLTKDRGVAASAGSSGENKKAKPVPWVEKYRPKCVDEVAFQEEVVAVLKKSLEGADLPNLLFYGPPGTGKTSTILAAARELFGPELFRLRVLELNASDERGIQVVREKVKNFAQLTVSGSRSDGKPCPPFKIVILDEADSMTSAAQAALRRTMEKESKTTRFCLICNYVSRIIEPLTSRCSKFRFKPLSDKIQQQRLLDIAKKENVKISDEGIAYLVKVSEGDLRKAITFLQSATRLTGGKEITEKVITDIAGVIPAEKIDGVFAACQSGSFDKLEAVVKDLIDEGHAATQLVNQLHDVVVENNLSDKQKSIITEKLAEVDKCLADGADEHLQLISLCATVMQQLSQNC

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Rabbit
100
Xenopus
91
Sheep
77
Zebrafish
73
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P35249 作为底物

Site PTM Type Enzyme
M1 Acetylation
K6 Acetylation
K6 Methylation
K6 Ubiquitination
S9 Phosphorylation
S11 Phosphorylation
T12 Phosphorylation
K13 Acetylation
K13 Ubiquitination
T17 Phosphorylation
K18 Ubiquitination
R20 Methylation
S25 Phosphorylation
S28 Phosphorylation
S29 Phosphorylation
K34 Ubiquitination
K36 Ubiquitination
K43 Ubiquitination
K47 Ubiquitination
K63 Ubiquitination
K64 Ubiquitination
K84 Ubiquitination
R114 Methylation
K124 Ubiquitination
K139 Ubiquitination
T166 Phosphorylation
T174 Phosphorylation
S184 Phosphorylation
T191 Phosphorylation
S192 Phosphorylation
K200 Ubiquitination
K205 Ubiquitination
K216 Acetylation
K216 Ubiquitination
K217 Acetylation
K221 Ubiquitination
K232 Ubiquitination
K240 Ubiquitination
K255 Ubiquitination
K260 Ubiquitination
S285 Phosphorylation
K288 Ubiquitination
K294 Ubiquitination
K322 Ubiquitination
K324 Ubiquitination
K330 Ubiquitination

研究背景

功能:

The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. This subunit may be involved in the elongation of the multiprimed DNA template.

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Heterotetramer of subunits RFC2, RFC3, RFC4 and RFC5 that can form a complex either with RFC1 or with RAD17. The former interacts with PCNA in the presence of ATP, while the latter has ATPase activity but is not stimulated by PCNA.

蛋白家族:

Belongs to the activator 1 small subunits family.

研究领域

· Genetic Information Processing > Replication and repair > DNA replication.

· Genetic Information Processing > Replication and repair > Nucleotide excision repair.

· Genetic Information Processing > Replication and repair > Mismatch repair.

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