Kallikrein 7 Antibody - #DF7384

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产品: Kallikrein 7 抗体
货号: DF7384
描述: Rabbit polyclonal antibody to Kallikrein 7
应用: WB IHC
反应: Human, Mouse
分子量: 27kDa; 28kD(Calculated).
蛋白号: P49862
RRID: AB_2839322

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 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
克隆:
Polyclonal
特异性:
Kallikrein 7 Antibody detects endogenous levels of total Kallikrein 7.
RRID:
AB_2839322
引用格式: Affinity Biosciences Cat# DF7384, RRID:AB_2839322.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Chymotryptic stratum corneum; hK 7; hK7; hSCCE; Kallikrein 7 (chymotryptic stratum corneum); Kallikrein related peptidase 7; Kallikrein-7; Kallikrein7; KLK 7; Klk7; KLK7_HUMAN; Protease serine 6; PRSS 6; PRSS6; SCCE; Serine protease 6; Signal protein; Stratum corneum chymotryptic enzyme;

抗原和靶标

免疫原:
Uniprot:

P49862(KLK7_HUMAN) >>Visit HPA database.

基因/基因ID:
KLK7(5650)
表达:
P49862 KLK7_HUMAN:

Abundantly expressed in the skin and is expressed by keratinocytes in the epidermis. Also expressed in the brain, mammary gland, cerebellum, spinal cord and kidney. Lower levels in salivary glands, uterus, thymus, thyroid, placenta, trachea and testis. Up-regulated in ovarian carcinoma, especially late-stage serous carcinoma, compared with normal ovaries and benign adenomas (at protein level).

描述:
This gene encodes a member of the kallikrein subfamily of serine proteases. These enzymes have diverse physiological functions and many kallikrein genes are biomarkers for cancer. The encoded protein has chymotrypsin-like activity and plays a role in the proteolysis of intercellular cohesive structures that precedes desquamation, the shedding of the outermost layer of the epidermis. The encoded protein may play a role in cancer invasion and metastasis, and increased expression of this gene is associated with unfavorable prognosis and progression of several types of cancer. Polymorphisms in this gene may play a role in the development of atopic dermatitis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, which is one of fifteen kallikrein subfamily members located in a gene cluster on chromosome 19.
序列:
MARSLLLPLQILLLSLALETAGEEAQGDKIIDGAPCARGSHPWQVALLSGNQLHCGGVLVNERWVLTAAHCKMNEYTVHLGSDTLGDRRAQRIKASKSFRHPGYSTQTHVNDLMLVKLNSQARLSSMVKKVRLPSRCEPPGTTCTVSGWGTTTSPDVTFPSDLMCVDVKLISPQDCTKVYKDLLENSMLCAGIPDSKKNACNGDSGGPLVCRGTLQGLVSWGTFPCGQPNDPGVYTQVCKFTKWINDTMKKHR

翻译修饰 - P49862 作为底物

Site PTM Type Enzyme
S120 Phosphorylation
S125 Phosphorylation
S161 Phosphorylation
S196 Phosphorylation

研究背景

功能:

May catalyze the degradation of intercellular cohesive structures in the cornified layer of the skin in the continuous shedding of cells from the skin surface. Specific for amino acid residues with aromatic side chains in the P1 position. Cleaves insulin A chain at '14-Tyr-|-Gln-15' and insulin B chain at '6-Leu-|-Cys-7', '16-Tyr-|-Leu-17', '25-Phe-|-Tyr-26' and '26-Tyr-|-Thr-27'. Could play a role in the activation of precursors to inflammatory cytokines.

细胞定位:

Secreted.
Note: In ovarian carcinoma, secreted and also observed at the apical membrane and in cytoplasm at the invasive front.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Abundantly expressed in the skin and is expressed by keratinocytes in the epidermis. Also expressed in the brain, mammary gland, cerebellum, spinal cord and kidney. Lower levels in salivary glands, uterus, thymus, thyroid, placenta, trachea and testis. Up-regulated in ovarian carcinoma, especially late-stage serous carcinoma, compared with normal ovaries and benign adenomas (at protein level).

蛋白家族:

Belongs to the peptidase S1 family. Kallikrein subfamily.

文献引用

1). Therapeutic effects of quinine in a mouse model of atopic dermatitis. Molecular Medicine Reports, 2021 (PubMed: 34240224) [IF=3.4]

Application: WB    Species: Mice    Sample: skin tissue

Figure 4. Effects of quinine on protein and mRNA expression in AD-like mice. (A) Protein expression of FLG and KLK7 evaluated via western blotting. (B) Relative ratio of FLG/GAPDH. (C) Relative ratio of KLK7/GAPDH. (D) Protein expression of FLG evaluated via immunohistochemistry. (E) Protein expression of KLK7 evaluated via immunohistochemistry. mRNA expression of (F) IκBα, (G) IKKα and (H) NF-κB determined via reverse transcription-quantitative PCR analysis. Data are presented as the mean ± standard deviation. *P<0.05, **P<0.001 vs. control; #P<0.05, ##P<0.001 vs. AD. AD, atopic dermatitis; AD + Q, AD group treated with quinine; C + Q, control group treated with quinine; FLG, filaggrin; KLK7, kallikrein-7; IKKα, IκB kinase α.

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