产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF7396, RRID:AB_2839334.
展开/折叠
CDC 46; CDC46; CDC46 homolog; Cell division cycle 46; DNA replication licensing factor; DNA replication licensing factor MCM 5; DNA replication licensing factor MCM5; MCM 5; MCM5; MCM5_HUMAN; MGC5315; Minichromosome maintenance complex component 5; Minichromosome maintenance deficient (S. cerevisiae) 5; Minichromosome maintenance deficient 5; Minichromosome maintenance deficient protein 5; P1 CDC46; P1-CDC46;
抗原和靶标
- P33992 MCM5_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSGFDDPGIFYSDSFGGDAQADEGQARKSQLQRRFKEFLRQYRVGTDRTGFTFKYRDELKRHYNLGEYWIEVEMEDLASFDEDLADYLYKQPAEHLQLLEEAAKEVADEVTRPRPSGEEVLQDIQVMLKSDASPSSIRSLKSDMMSHLVKIPGIIIAASAVRAKATRISIQCRSCRNTLTNIAMRPGLEGYALPRKCNTDQAGRPKCPLDPYFIMPDKCKCVDFQTLKLQELPDAVPHGEMPRHMQLYCDRYLCDKVVPGNRVTIMGIYSIKKFGLTTSRGRDRVGVGIRSSYIRVLGIQVDTDGSGRSFAGAVSPQEEEEFRRLAALPNVYEVISKSIAPSIFGGTDMKKAIACLLFGGSRKRLPDGLTRRGDINLLMLGDPGTAKSQLLKFVEKCSPIGVYTSGKGSSAAGLTASVMRDPSSRNFIMEGGAMVLADGGVVCIDEFDKMREDDRVAIHEAMEQQTISIAKAGITTTLNSRCSVLAAANSVFGRWDETKGEDNIDFMPTILSRFDMIFIVKDEHNEERDVMLAKHVITLHVSALTQTQAVEGEIDLAKLKKFIAYCRVKCGPRLSAEAAEKLKNRYIIMRSGARQHERDSDRRSSIPITVRQLEAIVRIAEALSKMKLQPFATEADVEEALRLFQVSTLDAALSGTLSGVEGFTSQEDQEMLSRIEKQLKRRFAIGSQVSEHSIIKDFTKQKYPEHAIHKVLQLMLRRGEIQHRMQRKVLYRLK
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P33992 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| S2 | Phosphorylation | Uniprot | |
| S12 | Phosphorylation | Uniprot | |
| S14 | Phosphorylation | Uniprot | |
| K36 | Ubiquitination | Uniprot | |
| K54 | Ubiquitination | Uniprot | |
| K129 | Ubiquitination | Uniprot | |
| S130 | Phosphorylation | Uniprot | |
| S133 | Phosphorylation | Uniprot | |
| K141 | Ubiquitination | Uniprot | |
| K150 | Ubiquitination | Uniprot | |
| S159 | Phosphorylation | Uniprot | |
| K206 | Ubiquitination | Uniprot | |
| Y212 | Phosphorylation | Uniprot | |
| K218 | Ubiquitination | Uniprot | |
| K220 | Acetylation | Uniprot | |
| K220 | Ubiquitination | Uniprot | |
| K228 | Ubiquitination | Uniprot | |
| Y248 | Phosphorylation | Uniprot | |
| R251 | Methylation | Uniprot | |
| K256 | Ubiquitination | Uniprot | |
| Y269 | Phosphorylation | Uniprot | |
| K273 | Ubiquitination | Uniprot | |
| S279 | Phosphorylation | Uniprot | |
| R280 | Methylation | Uniprot | |
| T303 | Phosphorylation | Uniprot | |
| S315 | Phosphorylation | Uniprot | |
| Y332 | Phosphorylation | Uniprot | |
| S336 | Phosphorylation | Uniprot | |
| K337 | Ubiquitination | Uniprot | |
| S338 | Phosphorylation | Uniprot | |
| S342 | Phosphorylation | Uniprot | |
| K350 | Ubiquitination | Uniprot | |
| K351 | Ubiquitination | Uniprot | |
| S361 | Phosphorylation | Uniprot | |
| K363 | Ubiquitination | Uniprot | |
| T385 | Phosphorylation | Uniprot | |
| K387 | Acetylation | Uniprot | |
| K387 | Ubiquitination | Uniprot | |
| K392 | Acetylation | Uniprot | |
| K392 | Ubiquitination | Uniprot | |
| K396 | Acetylation | Uniprot | |
| K396 | Ubiquitination | Uniprot | |
| Y403 | Phosphorylation | Uniprot | |
| K407 | Ubiquitination | Uniprot | |
| S423 | Phosphorylation | Uniprot | |
| S424 | Phosphorylation | Uniprot | |
| K471 | Ubiquitination | Uniprot | |
| C482 | S-Nitrosylation | Uniprot | |
| K499 | Ubiquitination | Uniprot | |
| R528 | Methylation | Uniprot | |
| K561 | Ubiquitination | Uniprot | |
| K581 | Acetylation | Uniprot | |
| K581 | Ubiquitination | Uniprot | |
| K583 | Ubiquitination | Uniprot | |
| S604 | Phosphorylation | Uniprot | |
| S605 | Phosphorylation | Uniprot | |
| K625 | Ubiquitination | Uniprot | |
| K627 | Ubiquitination | Uniprot | |
| T633 | Phosphorylation | O14757 (CHEK1) | Uniprot |
| S693 | Phosphorylation | Uniprot | |
| K696 | Acetylation | Uniprot | |
| K696 | Ubiquitination | Uniprot | |
| K702 | Ubiquitination | Uniprot |
研究背景
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (By similarity). Interacts with MCMBP.
Nucleus. Cytoplasm>Cytosol.
Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5 (Probable). Interacts with ANKRD17.
Belongs to the MCM family.
研究领域
· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)
· Genetic Information Processing > Replication and repair > DNA replication.
限制条款
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