产品: RPLP0 抗体
货号: DF7397
描述: Rabbit polyclonal antibody to RPLP0
应用: WB IHC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 34kDa; 34kD(Calculated).
蛋白号: P05388
RRID: AB_2839335

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(83%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(92%)
克隆:
Polyclonal
特异性:
RPLP0 Antibody detects endogenous levels of total RPLP0.
RRID:
AB_2839335
引用格式: Affinity Biosciences Cat# DF7397, RRID:AB_2839335.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

36B4; 60S acidic ribosomal protein P0; 60S ribosomal protein L10E; Acidic ribosomal phosphoprotein P0; Arbp; L10E; LP0; MGC107165; MGC107166; MGC111226; MGC88175; OTTMUSP00000015585; P0; PRLP0; Ribosomal phosphoprotein, acidic, P0; Ribosomal protein P0; Ribosomal protein, large, P0; RLA0_HUMAN; rplP0; RPP0;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
描述:
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein, which is the functional equivalent of the E. coli L10 ribosomal protein, belongs to the L10P family of ribosomal proteins. It is a neutral phosphoprotein with a C-terminal end that is nearly identical to the C-terminal ends of the acidic ribosomal phosphoproteins P1 and P2. The P0 protein can interact with P1 and P2 to form a pentameric complex consisting of P1 and P2 dimers, and a P0 monomer. The protein is located in the cytoplasm. Transcript variants derived from alternative splicing exist; they encode the same protein. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
序列:
MPREDRATWKSNYFLKIIQLLDDYPKCFIVGADNVGSKQMQQIRMSLRGKAVVLMGKNTMMRKAIRGHLENNPALEKLLPHIRGNVGFVFTKEDLTEIRDMLLANKVPAAARAGAIAPCEVTVPAQNTGLGPEKTSFFQALGITTKISRGTIEILSDVQLIKTGDKVGASEATLLNMLNISPFSFGLVIQQVFDNGSIYNPEVLDITEETLHSRFLEGVRNVASVCLQIGYPTVASVPHSIINGYKRVLALSVETDYTFPLAEKVKAFLADPSAFVAAAPVAAATTAAPAAAAAPAKVEAKEESEESDEDMGFGLFD

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
92
Zebrafish
83
Horse
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P05388 作为底物

Site PTM Type Enzyme
R6 Methylation
T8 Phosphorylation
K10 Acetylation
K10 Methylation
K10 Ubiquitination
K16 Methylation
K16 Ubiquitination
Y24 Phosphorylation
K26 Ubiquitination
C27 S-Nitrosylation
S37 Phosphorylation
K38 Ubiquitination
K50 Ubiquitination
K57 Ubiquitination
T59 Phosphorylation
R66 Methylation
K77 Acetylation
K77 Ubiquitination
K92 Ubiquitination
K106 Acetylation
K106 Ubiquitination
R112 Methylation
C119 S-Nitrosylation
T122 Phosphorylation
T128 Phosphorylation
K134 Ubiquitination
T135 Phosphorylation
S136 Phosphorylation
K146 Ubiquitination
S148 Phosphorylation
T151 Phosphorylation
S156 Phosphorylation
K162 Ubiquitination
K166 Ubiquitination
K246 Acetylation
K246 Ubiquitination
S252 Phosphorylation
Y257 Phosphorylation
K264 Ubiquitination
K266 Ubiquitination
S273 Phosphorylation
T285 Phosphorylation
T286 Phosphorylation
K297 Sumoylation
K297 Ubiquitination
S304 Phosphorylation
S307 Phosphorylation

研究背景

功能:

Ribosomal protein P0 is the functional equivalent of E.coli protein L10.

细胞定位:

Nucleus. Cytoplasm.
Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs (PubMed:19188445, PubMed:17289661).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

P0 forms a pentameric complex by interaction with dimers of P1 and P2. Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs. Interacts with APEX1. Interacts with FMR1 isoform 6.

蛋白家族:

Belongs to the universal ribosomal protein uL10 family.

研究领域

· Genetic Information Processing > Translation > Ribosome.

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