产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF7410, RRID:AB_2839348.
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DKFZp667H044; FLJ20353; Histone lysine N methyltransferase NSD3; Histone-lysine N-methyltransferase NSD3; MGC126766; MGC142029; NSD 3; NSD3_HUMAN; Nuclear set domain containing 3; Nuclear SET domain containing protein 3; Nuclear SET domain-containing protein 3; pp14328; Protein whistle; Whistle; WHSC1 like protein 1; WHSC1-like 1 isoform 9 with methyltransferase activity to lysine; WHSC1-like protein 1; WHSC1L1; WHSC1L1 protein; Wolf Hirschhorn syndrome candidate 1 like 1; Wolf-Hirschhorn syndrome candidate 1-like protein 1;
抗原和靶标
Highly expressed in brain, heart and skeletal muscle. Expressed at lower level in liver and lung.
- Q9BZ95 NSD3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDFSFSFMQGIMGNTIQQPPQLIDSANIRQEDAFDNNSDIAEDGGQTPYEATLQQGFQYPATTEDLPPLTNGYPSSISVYETQTKYQSYNQYPNGSANGFGAVRNFSPTDYYHSEIPNTRPHEILEKPSPPQPPPPPSVPQTVIPKKTGSPEIKLKITKTIQNGRELFESSLCGDLLNEVQASEHTKSKHESRKEKRKKSNKHDSSRSEERKSHKIPKLEPEEQNRPNERVDTVSEKPREEPVLKEEAPVQPILSSVPTTEVSTGVKFQVGDLVWSKVGTYPWWPCMVSSDPQLEVHTKINTRGAREYHVQFFSNQPERAWVHEKRVREYKGHKQYEELLAEATKQASNHSEKQKIRKPRPQRERAQWDIGIAHAEKALKMTREERIEQYTFIYIDKQPEEALSQAKKSVASKTEVKKTRRPRSVLNTQPEQTNAGEVASSLSSTEIRRHSQRRHTSAEEEEPPPVKIAWKTAAARKSLPASITMHKGSLDLQKCNMSPVVKIEQVFALQNATGDGKFIDQFVYSTKGIGNKTEISVRGQDRLIISTPNQRNEKPTQSVSSPEATSGSTGSVEKKQQRRSIRTRSESEKSTEVVPKKKIKKEQVETVPQATVKTGLQKGASEISDSCKPLKKRSRASTDVEMTSSAYRDTSDSDSRGLSDLQVGFGKQVDSPSATADADVSDVQSMDSSLSRRGTGMSKKDTVCQICESSGDSLIPCEGECCKHFHLECLGLASLPDSKFICMECKTGQHPCFSCKVSGKDVKRCSVGACGKFYHEACVRKFPTAIFESKGFRCPQHCCSACSMEKDIHKASKGRMMRCLRCPVAYHSGDACIAAGSMLVSSYILICSNHSKRSSNSSAVNVGFCFVCARGLIVQDHSDPMFSSYAYKSHYLLNESNRAELMKLPMIPSSSASKKKCEKGGRLLCCESCPASFHPECLSIEMPEGCWNCNDCKAGKKLHYKQIVWVKLGNYRWWPAEICNPRSVPLNIQGLKHDLGDFPVFFFGSHDYYWVHQGRVFPYVEGDKSFAEGQTSINKTFKKALEEAAKRFQELKAQRESKEALEIEKNSRKPPPYKHIKANKVIGKVQIQVADLSEIPRCNCKPADENPCGLESECLNRMLQYECHPQVCPAGDRCQNQCFTKRLYPDAEIIKTERRGWGLRTKRSIKKGEFVNEYVGELIDEEECRLRIKRAHENSVTNFYMLTVTKDRIIDAGPKGNYSRFMNHSCNPNCETQKWTVNGDVRVGLFALCDIPAGMELTFNYNLDCLGNGRTECHCGADNCSGFLGVRPKSACASTNEEKAKNAKLKQKRRKIKTEPKQMHEDYCFQCGDGGELVMCDKKDCPKAYHLLCLNLTQPPYGKWECPWHQCDECSSAAVSFCEFCPHSFCKDHEKGALVPSALEGRLCCSEHDPMAPVSPEYWSKIKCKWESQDHGEEVKE
种属预测
score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9BZ95 作为底物
Site | PTM Type | Enzyme | Source |
---|---|---|---|
S107 | Phosphorylation | Uniprot | |
K127 | Ubiquitination | Uniprot | |
S129 | Phosphorylation | Uniprot | |
S150 | Phosphorylation | Uniprot | |
K159 | Ubiquitination | Uniprot | |
S183 | Phosphorylation | Uniprot | |
T186 | Phosphorylation | Uniprot | |
K187 | Ubiquitination | Uniprot | |
S188 | Phosphorylation | Uniprot | |
K218 | Sumoylation | Uniprot | |
K245 | Sumoylation | Uniprot | |
K334 | Ubiquitination | Uniprot | |
K345 | Ubiquitination | Uniprot | |
Y390 | Phosphorylation | Uniprot | |
Y394 | Phosphorylation | Uniprot | |
S424 | Phosphorylation | Uniprot | |
T428 | Phosphorylation | Uniprot | |
T433 | Phosphorylation | Uniprot | |
T456 | Phosphorylation | Uniprot | |
S457 | Phosphorylation | Uniprot | |
K467 | Sumoylation | Uniprot | |
K467 | Ubiquitination | Uniprot | |
K471 | Ubiquitination | Uniprot | |
K477 | Methylation | Uniprot | |
K477 | Ubiquitination | Uniprot | |
S478 | Phosphorylation | Uniprot | |
K487 | Ubiquitination | Uniprot | |
S489 | Phosphorylation | Uniprot | |
K494 | Ubiquitination | Uniprot | |
S498 | Phosphorylation | Uniprot | |
Y524 | Phosphorylation | Uniprot | |
K527 | Ubiquitination | Uniprot | |
K532 | Acetylation | Uniprot | |
K532 | Sumoylation | Uniprot | |
K532 | Ubiquitination | Uniprot | |
S546 | Phosphorylation | Uniprot | |
T547 | Phosphorylation | Uniprot | |
K554 | Acetylation | Uniprot | |
K554 | Ubiquitination | Uniprot | |
T556 | Phosphorylation | Uniprot | |
S558 | Phosphorylation | Uniprot | |
S560 | Phosphorylation | Uniprot | |
S561 | Phosphorylation | Uniprot | |
S568 | Phosphorylation | Uniprot | |
T569 | Phosphorylation | Uniprot | |
S571 | Phosphorylation | Uniprot | |
S580 | Phosphorylation | Uniprot | |
T583 | Phosphorylation | Uniprot | |
S585 | Phosphorylation | Uniprot | |
S587 | Phosphorylation | Uniprot | |
S590 | Phosphorylation | Uniprot | |
K601 | Sumoylation | Uniprot | |
T611 | Phosphorylation | Uniprot | |
K628 | Sumoylation | Uniprot | |
S634 | Phosphorylation | Uniprot | |
S637 | Phosphorylation | Uniprot | |
T638 | Phosphorylation | Uniprot | |
S655 | Phosphorylation | Uniprot | |
S659 | Phosphorylation | Uniprot | |
K667 | Methylation | Uniprot | |
S671 | Phosphorylation | Uniprot | |
K781 | Acetylation | Uniprot | |
K790 | Acetylation | Uniprot | |
S889 | Phosphorylation | Uniprot | |
Y891 | Phosphorylation | Uniprot | |
S896 | Phosphorylation | Uniprot | |
K914 | Acetylation | Uniprot | |
K915 | Acetylation | Uniprot | |
Y960 | Phosphorylation | Uniprot | |
Y971 | Phosphorylation | Uniprot | |
K1058 | Sumoylation | Uniprot | |
K1065 | Ubiquitination | Uniprot | |
Y1073 | Phosphorylation | Uniprot | |
K1074 | Ubiquitination | Uniprot | |
K1077 | Acetylation | Uniprot | |
K1080 | Acetylation | Uniprot | |
K1141 | Ubiquitination | Uniprot | |
K1151 | Sumoylation | Uniprot | |
K1167 | Ubiquitination | Uniprot | |
K1215 | Ubiquitination | Uniprot | |
K1391 | Ubiquitination | Uniprot | |
S1406 | Phosphorylation | Uniprot | |
S1415 | Phosphorylation | Uniprot |
研究背景
Histone methyltransferase. Preferentially dimethylates 'Lys-4' and 'Lys-27' of histone H3 forming H3K2me2 and H3K27me2. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation, while 'Lys-27' is a mark for transcriptional repression.
Nucleus. Chromosome.
Highly expressed in brain, heart and skeletal muscle. Expressed at lower level in liver and lung.
Interacts with BRD4.
Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. SET2 subfamily.
研究领域
· Metabolism > Amino acid metabolism > Lysine degradation.
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