产品: ICAM1 抗体
货号: DF7413
来源: Rabbit
应用: WB, IHC, IF/ICC, ELISA(peptide)
反应: Human
分子量: 57kD; 58kD(Calculated).
蛋白号: P05362
RRID: AB_2839351

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货期: 当天发货

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human
克隆:
Polyclonal
特异性:
ICAM1 Antibody detects endogenous levels of total ICAM1.
RRID:
AB_2839351
引用格式: Affinity Biosciences Cat# DF7413, RRID:AB_2839351.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Antigen identified by monoclonal antibody BB2; BB 2; BB2; CD 54; CD_antigen=CD54; CD54; Cell surface glycoprotein P3.58; Human rhinovirus receptor; ICAM 1; ICAM-1; ICAM1; ICAM1_HUMAN; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; Intercellular adhesion molecule 1; Major group rhinovirus receptor; MALA 2; MALA2; MyD 10; MyD10; P3.58; Surface antigen of activated B cells, BB2;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
蛋白描述:
This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor.
蛋白序列:
MAPSSPRPALPALLVLLGALFPGPGNAQTSVSPSKVILPRGGSVLVTCSTSCDQPKLLGIETPLPKKELLLPGNNRKVYELSNVQEDSQPMCYSNCPDGQSTAKTFLTVYWTPERVELAPLPSWQPVGKNLTLRCQVEGGAPRANLTVVLLRGEKELKREPAVGEPAEVTTTVLVRRDHHGANFSCRTELDLRPQGLELFENTSAPYQLQTFVLPATPPQLVSPRVLEVDTQGTVVCSLDGLFPVSEAQVHLALGDQRLNPTVTYGNDSFSAKASVSVTAEDEGTQRLTCAVILGNQSQETLQTVTIYSFPAPNVILTKPEVSEGTEVTVKCEAHPRAKVTLNGVPAQPLGPRAQLLLKATPEDNGRSFSCSATLEVAGQLIHKNQTRELRVLYGPRLDERDCPGNWTWPENSQQTPMCQAWGNPLPELKCLKDGTFPLPIGESVTVTRDLEGTYLCRARSTQGEVTRKVTVNVLSPRYEIVIITVVAAAVIMGTAGLSTYLYNRQRKIKKYRLQQAQKGTPMKPNTQATPP

翻译修饰 - P05362 作为底物

Site PTM Type Enzyme
S43 Phosphorylation
T62 O-Glycosylation
T62 Phosphorylation
N130 N-Glycosylation
N145 N-Glycosylation
N183 N-Glycosylation
N202 N-Glycosylation
Y207 Phosphorylation
T211 Phosphorylation
T217 Phosphorylation
S223 Phosphorylation
N267 N-Glycosylation
S275 Phosphorylation
N296 N-Glycosylation
S323 Phosphorylation
K359 Ubiquitination
N385 N-Glycosylation
Y501 Phosphorylation
Y512 Phosphorylation
K519 Ubiquitination
T521 Phosphorylation
K524 Ubiquitination
T527 Phosphorylation
T530 Phosphorylation

研究背景

功能:

ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation.

(Microbial infection) Acts as a receptor for major receptor group rhinovirus A-B capsid proteins.

(Microbial infection) Acts as a receptor for Coxsackievirus A21 capsid proteins.

(Microbial infection) Upon Kaposi's sarcoma-associated herpesvirus/HHV-8 infection, is degraded by viral E3 ubiquitin ligase MIR2, presumably to prevent lysis of infected cells by cytotoxic T-lymphocytes and NK cell.

翻译修饰:

Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.

细胞定位:

Membrane>Single-pass type I membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Homodimer (Probable). Interacts with MUC1 and promotes cell aggregation in epithelial cells. Interacts with ARHGEF26/SGEF. Interacts (on T cell side) with CD81, CD247 and CD9 at immunological synapses between antigen-presenting cells and T cells.

(Microbial infection) Interacts with major receptor group rhinovirus A-B capsid proteins.

(Microbial infection) Interacts with Coxsackievirus A21 capsid proteins.

蛋白家族:

Belongs to the immunoglobulin superfamily. ICAM family.

研究领域

· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs).   (View pathway)

· Environmental Information Processing > Signal transduction > TNF signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Parasitic > African trypanosomiasis.

· Human Diseases > Infectious diseases: Parasitic > Malaria.

· Human Diseases > Infectious diseases: Bacterial > Staphylococcus aureus infection.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Human Diseases > Immune diseases > Rheumatoid arthritis.

· Human Diseases > Cardiovascular diseases > Viral myocarditis.

· Organismal Systems > Immune system > Natural killer cell mediated cytotoxicity.   (View pathway)

· Organismal Systems > Immune system > Leukocyte transendothelial migration.   (View pathway)

文献引用

1). Zhao M et al. HIF-1α/JMJD1A signaling regulates inflammation and oxidative stress following hyperglycemia and hypoxia-induced vascular cell injury. Cell Mol Biol Lett 2021 Sep 3;26(1):40. (PubMed: 34479471) [IF=8.702]

2). Ge MH et al. Zinc attenuates ferroptosis and promotes functional recovery in contusion spinal cord injury by activating Nrf2/GPX4 defense pathway. CNS Neurosci Ther 2021 May 5;27(9):1023-1040. (PubMed: 33951302) [IF=7.035]

Application: WB    Species: Mouse    Sample: Spinal cord

FIGURE 7 Zinc can reduce the inflammation of damaged parts by inhibiting ferroptosis. (A) The expressions of TNF-α, IL-6, IL-1β, and ICAM-1 were evaluated by Western blotting at 3 days post-SCI in each group (n = 6). (B) Quantification of TNF-α, IL-6, IL-1β, and ICAM-1 expressions (n = 6, all the data are expressed as means ± SD, two-way ANOVA followed by Tukey's post hoc test was applied). (C–F) Immunofluorescence staining was used to detect the level of TNF-α, IL-6, IL-1β, and ICAM-1 from each group (n = 6, scale bar = 50 µm). (G) Statistical analysis of immunofluorescence staining for positive expression of TNF-α, IL-6, IL-1β, and ICAM-1 in nerve cells from each group (n = 6, all the data are expressed as means ± SD, two-way ANOVA followed by Tukey's post hoc test was applied). * means p < 0.05; **means p < 0.01; and *** means p < 0.001

3). Yang F et al. Karyopherin α 2 promotes proliferation, migration and invasion through activating NF-κB/p65 signaling pathways in melanoma cells. Life Sci 2020 Mar 31:117611 (PubMed: 32243925) [IF=6.780]

Application: WB    Species: Mice    Sample: melanoma cells

Fig. 4. KPNA2 activated NF-κB/p65 signaling pathway in melanoma cells. The expression of NF-κB p65, COX-2, ICAM-1, iNOS, MCP1, and p65 (nucleus) was analyzed by western blot in the KPNA2-overexpressing (A, B) or KPNA2-silenced cells (F, G). The nuclear translocation of p65 (nucleus) was detected by immunofluorescence assay in the KPNA2-overexpressing (C, D) or KPNA2-silenced cells (H, I). The NF-κB binding activity was analyzed by EMSA in the KPNA2- overexpressing (E) or KPNA2-silenced cells (J). Parental, blank control group; NC, negative control group; OV-KPNA2, KPNA2 overexpressed group; siRNA1-KPNA2, KPNA2-1 silencing group; siRNA2-KPNA2, KPNA2-2 silencing group. The results were obtained in three independent experiments. Mean values were compared by One-way ANOVA. (**p < 0.01, ***p < 0.001, ****p < 0.001).

4). Chen X et al. Galectin-3 exacerbates ox-LDL-mediated endothelial injury by inducing inflammation via integrin β1-RhoA-JNK signaling activation. J Cell Physiol 2018 Dec 10 (PubMed: 30536538) [IF=6.513]

Application: WB    Species: human    Sample: HUVECs cells

FIGURE 6| Gal‐3 induced expression of inflammatory factors promoted HUVECs injury via integrin β1‐RhoA‐JNK pathway. HUVECs,exposing to the ox‐LDL alone or in combination with Gal‐3 were further treated with integrin β1‐siRNA or JNK inhibitor (SP600125). The total and phosphorylated expression of p65,IKKα and IKKβ after the above treatment was examined by WB and demonstrated by histogram. (a and b).The levels of inflammatory cytokines and chemokines were then measured by ELISA. (c and d) The expression of adhesion molecules (ICAM‐1 and VCAM‐1) was detected by WB (e). *p < 0.05, **p < 0.01

5). Huang S et al. Nepeta angustifolia attenuates responses to vascular inflammation in high glucose-induced human umbilical vein endothelial cells through heme oxygenase-1 induction. J Ethnopharmacol 2019 Mar 1;231:187-196 (PubMed: 30419276) [IF=5.195]

6). Liang PL et al. Guang Chen Pi (the pericarp of Citrus reticulata Blanco's cultivars 'Chachi') inhibits macrophage-derived foam cell formation. J Ethnopharmacol 2022 Jul 15;293:115328. (PubMed: 35489660) [IF=5.195]

7). Smith AO et al. Novel Strategy for Isolation of Mice Bone Marrow–Derived Endothelial Cells (BMECs). Stem Cell Res Ther 2021 May 3;12(1):267. (PubMed: 33941266) [IF=5.116]

8). Cai W et al. Uric Acid Induces Endothelial Dysfunction by Activating the HMGB1/RAGE Signaling Pathway. Biomed Res Int 2017;2017:4391920 (PubMed: 28116308) [IF=3.246]

Application: WB    Species: human    Sample:


9). Wang X et al. Therapeutic effect and mechanism of 4‑phenyl butyric acid on renal ischemia‑reperfusion injury in mice. Exp Ther Med 2022 Feb;23(2):144. (PubMed: 35069825) [IF=2.751]

10). Chai Y et al. Protective Effects of Angong Niuhuang Pill on Early Atherosclerosis in ApoE-/- Mice by Reducing the Inflammatory Response. Evid Based Complement Alternat Med 2019 May 20;2019:9747212 (PubMed: 31236126) [IF=2.650]

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