产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF7414, RRID:AB_2839352.
展开/折叠
AREB 6; BZP; Delta crystallin enhancer binding factor 1; DELTA EF1; FECD6; MGC133261; Negative regulator of IL 2; Negative regulator of IL2; NIL 2 A; NIL 2 A zinc finger protein; NIL 2A; NIL-2-A zinc finger protein; NIL2A; Posterior polymorphous corneal dystrophy 3; PPCD3; Represses interleukin 2 expression; TCF 8; TCF-8; TCF8; Transcription factor 8 (represses interleukin 2 expression); Transcription factor 8; ZEB 1; ZEB; ZEB1; ZEB1_HUMAN; ZFHEP; ZFHX 1A; ZFHX1A; Zinc finger E box binding homeobox 1; Zinc finger E-box-binding homeobox 1; Zinc finger homeodomain enhancer binding protein;
抗原和靶标
Colocalizes with SMARCA4/BRG1 in E-cadherin-negative cells from established lines, and stroma of normal colon as well as in de-differentiated epithelial cells at the invasion front of colorectal carcinomas (at protein level). Expressed in heart and skeletal muscle, but not in liver, spleen, or pancreas.
- P37275 ZEB1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MADGPRCKRRKQANPRRNNVTNYNTVVETNSDSDDEDKLHIVEEESVTDAADCEGVPEDDLPTDQTVLPGRSSEREGNAKNCWEDDRKEGQEILGPEAQADEAGCTVKDDECESDAENEQNHDPNVEEFLQQQDTAVIFPEAPEEDQRQGTPEASGHDENGTPDAFSQLLTCPYCDRGYKRFTSLKEHIKYRHEKNEDNFSCSLCSYTFAYRTQLERHMTSHKSGRDQRHVTQSGCNRKFKCTECGKAFKYKHHLKEHLRIHSGEKPYECPNCKKRFSHSGSYSSHISSKKCISLIPVNGRPRTGLKTSQCSSPSLSASPGSPTRPQIRQKIENKPLQEQLSVNQIKTEPVDYEFKPIVVASGINCSTPLQNGVFTGGGPLQATSSPQGMVQAVVLPTVGLVSPISINLSDIQNVLKVAVDGNVIRQVLENNQANLASKEQETINASPIQQGGHSVISAISLPLVDQDGTTKIIINYSLEQPSQLQVVPQNLKKENPVATNSCKSEKLPEDLTVKSEKDKSFEGGVNDSTCLLCDDCPGDINALPELKHYDLKQPTQPPPLPAAEAEKPESSVSSATGDGNLSPSQPPLKNLLSLLKAYYALNAQPSAEELSKIADSVNLPLDVVKKWFEKMQAGQISVQSSEPSSPEPGKVNIPAKNNDQPQSANANEPQDSTVNLQSPLKMTNSPVLPVGSTTNGSRSSTPSPSPLNLSSSRNTQGYLYTAEGAQEEPQVEPLDLSLPKQQGELLERSTITSVYQNSVYSVQEEPLNLSCAKKEPQKDSCVTDSEPVVNVIPPSANPINIAIPTVTAQLPTIVAIADQNSVPCLRALAANKQTILIPQVAYTYSTTVSPAVQEPPLKVIQPNGNQDERQDTSSEGVSNVEDQNDSDSTPPKKKMRKTENGMYACDLCDKIFQKSSSLLRHKYEHTGKRPHECGICKKAFKHKHHLIEHMRLHSGEKPYQCDKCGKRFSHSGSYSQHMNHRYSYCKREAEERDSTEQEEAGPEILSNEHVGARASPSQGDSDERESLTREEDEDSEKEEEEEDKEMEELQEEKECEKPQGDEEEEEEEEEVEEEEVEEAENEGEEAKTEGLMKDDRAESQASSLGQKVGESSEQVSEEKTNEA
种属预测
score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P37275 作为底物
Site | PTM Type | Enzyme | Source |
---|---|---|---|
S31 | Phosphorylation | Uniprot | |
S33 | Phosphorylation | Uniprot | |
T151 | Phosphorylation | Uniprot | |
S155 | Phosphorylation | Uniprot | |
T162 | Phosphorylation | Uniprot | |
S184 | Phosphorylation | Uniprot | |
K186 | Ubiquitination | Uniprot | |
T243 | Phosphorylation | Uniprot | |
S263 | Phosphorylation | Uniprot | |
S282 | Phosphorylation | Uniprot | |
S285 | Phosphorylation | Uniprot | |
S288 | Phosphorylation | Uniprot | |
S289 | Phosphorylation | Uniprot | |
S309 | Phosphorylation | Uniprot | |
S312 | Phosphorylation | Uniprot | |
S313 | Phosphorylation | Uniprot | |
S315 | Phosphorylation | Uniprot | |
S317 | Phosphorylation | Uniprot | |
S319 | Phosphorylation | Uniprot | |
S322 | Phosphorylation | Uniprot | |
T324 | Phosphorylation | Uniprot | |
K335 | Ubiquitination | Uniprot | |
S342 | Phosphorylation | Uniprot | |
K347 | Sumoylation | Uniprot | |
T443 | Phosphorylation | Uniprot | |
S447 | Phosphorylation | Uniprot | |
S455 | Phosphorylation | Uniprot | |
K493 | Sumoylation | Uniprot | |
K504 | Sumoylation | Uniprot | |
K504 | Ubiquitination | Uniprot | |
K507 | Ubiquitination | Uniprot | |
K515 | Sumoylation | Uniprot | |
K515 | Ubiquitination | Uniprot | |
S516 | Phosphorylation | Uniprot | |
S521 | Phosphorylation | Uniprot | |
S529 | Phosphorylation | Uniprot | |
S583 | Phosphorylation | Uniprot | |
S585 | Phosphorylation | Uniprot | |
K613 | Ubiquitination | Uniprot | |
S641 | Phosphorylation | Uniprot | |
S642 | Phosphorylation | Uniprot | |
S645 | Phosphorylation | Uniprot | |
S646 | Phosphorylation | Uniprot | |
S679 | Phosphorylation | Uniprot | |
S686 | Phosphorylation | Uniprot | |
S693 | Phosphorylation | Uniprot | |
S698 | Phosphorylation | Uniprot | |
S700 | Phosphorylation | Uniprot | |
S701 | Phosphorylation | Uniprot | |
T702 | Phosphorylation | Uniprot | |
S704 | Phosphorylation | Uniprot | |
S706 | Phosphorylation | Uniprot | |
T716 | Phosphorylation | Uniprot | |
S850 | Phosphorylation | Uniprot | |
S887 | Phosphorylation | Uniprot | |
S889 | Phosphorylation | Uniprot | |
T890 | Phosphorylation | Uniprot | |
T927 | Phosphorylation | Uniprot | |
S955 | Phosphorylation | Uniprot | |
S995 | Phosphorylation | Uniprot | |
T996 | Phosphorylation | Uniprot | |
S1016 | Phosphorylation | Uniprot | |
S1018 | Phosphorylation | Uniprot | |
S1022 | Phosphorylation | Uniprot | |
S1036 | Phosphorylation | Uniprot | |
S1100 | Phosphorylation | Uniprot | |
K1108 | Ubiquitination | Uniprot |
研究背景
Acts as a transcriptional repressor. Inhibits interleukin-2 (IL-2) gene expression. Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Positively regulates neuronal differentiation. Represses RCOR1 transcription activation during neurogenesis. Represses transcription by binding to the E box (5'-CANNTG-3'). Promotes tumorigenicity by repressing stemness-inhibiting microRNAs.
Nucleus.
Colocalizes with SMARCA4/BRG1 in E-cadherin-negative cells from established lines, and stroma of normal colon as well as in de-differentiated epithelial cells at the invasion front of colorectal carcinomas (at protein level). Expressed in heart and skeletal muscle, but not in liver, spleen, or pancreas.
Interacts (via N-terminus) with SMARCA4/BRG1.
Belongs to the delta-EF1/ZFH-1 C2H2-type zinc-finger family.
研究领域
· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.
· Human Diseases > Cancers: Overview > MicroRNAs in cancer.
· Human Diseases > Cancers: Specific types > Prostate cancer. (View pathway)
文献引用
Application: WB Species: human Sample: PLC/PRF/5 cells
Application: IF/ICC Species: human Sample: human cervical cancer tissues
Application: WB Species: human Sample: hypoxic cancer cells
Application: WB Species: human Sample: A549 cells
Application: WB Species: human Sample: A549/PTX cells
Application: WB Species: human Sample: SW480 and HCT116 cells
Application: IF/ICC Species: human Sample: SW480 and HCT116 cells
Application: WB Species: human Sample: EECs
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