| 产品: | GABARAP 抗体 |
| 货号: | DF7419 |
| 描述: | Rabbit polyclonal antibody to GABARAP |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Sheep, Rabbit, Dog, Xenopus |
| 分子量: | 13kDa; 14kD(Calculated). |
| 蛋白号: | O95166 |
| RRID: | AB_2839357 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF7419, RRID:AB_2839357.
展开/折叠
ATG8A; FLC 3B; FLC3B; FLJ25768; GABA(A) receptor associated protein; GABA(A) receptor-associated protein; GABARAP a; GABARAP; Gamma aminobutyric acid receptor associated protein; Gamma-aminobutyric acid receptor-associated protein; GBRAP_HUMAN; MGC120154; MGC120155; MM 46; MM46;
抗原和靶标
- O95166 GBRAP_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MKFVYKEEHPFEKRRSEGEKIRKKYPDRVPVIVEKAPKARIGDLDKKKYLVPSDLTVGQFYFLIRKRIHLRAEDALFFFVNNVIPPTSATMGQLYQEHHEEDFFLYIAYSDESVYGL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O95166 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K6 | Ubiquitination | Uniprot | |
| K13 | Ubiquitination | Uniprot | |
| K23 | Ubiquitination | Uniprot | |
| K24 | Ubiquitination | Uniprot | |
| R28 | Methylation | Uniprot | |
| K35 | Acetylation | Uniprot | |
| K35 | Ubiquitination | Uniprot | |
| Y49 | Phosphorylation | Uniprot | |
| T56 | Phosphorylation | Uniprot | |
| Y61 | Phosphorylation | Uniprot |
研究背景
Ubiquitin-like modifier that plays a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton. Involved in apoptosis. Involved in autophagy. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Through its interaction with the reticulophagy receptor TEX264, paticipates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover.
The precursor molecule is cleaved by ATG4B to form the cytosolic form, GABARAP-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, GABARAP-II.
Endomembrane system. Cytoplasm>Cytoskeleton. Golgi apparatus membrane. Cytoplasmic vesicle>Autophagosome. Cytoplasmic vesicle.
Note: Largely associated with intracellular membrane structures including the Golgi apparatus and postsynaptic cisternae. Colocalizes with microtubules (By similarity). Localizes also to discrete punctae along the ciliary axoneme (By similarity).
Heart, brain, placenta, liver, skeletal muscle, kidney and pancreas.
Interacts with GPHN and NSF (By similarity). Interacts with ATG3, ATG7, ATG13. Interacts with alpha- and beta-tubulin. Interacts with GABRG2. Interacts with RB1CC1. Interacts with ULK1. Interacts with CALR. Interacts with DDX47. Interacts with TP53INP1 and TP53INP2. Interacts with TBC1D5 and TBC1D25. Directly interacts with SQSTM1. Interacts with MAPK15. Interacts with TECPR2. Interacts with PCM1. Interacts with TRIM5 and TRIM21. Interacts with MEFV. Interacts with KIF21B (By similarity). Interacts with WDFY3; this interaction is required for WDFY3 recruitment to MAP1LC3B-positive p62/SQSTM1 bodies. Interacts with the reticulophagy receptor TEX264.
Belongs to the ATG8 family.
研究领域
· Cellular Processes > Transport and catabolism > Autophagy - other. (View pathway)
· Cellular Processes > Transport and catabolism > Autophagy - animal. (View pathway)
· Environmental Information Processing > Signal transduction > FoxO signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Apelin signaling pathway. (View pathway)
· Organismal Systems > Immune system > NOD-like receptor signaling pathway. (View pathway)
· Organismal Systems > Nervous system > GABAergic synapse.
限制条款
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