产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF7423, RRID:AB_2839361.
展开/折叠
cAMP response element modulator; cAMP responsive element modulator; cAMP-responsive element modulator; CREM; CREM-2; CREM_HUMAN; hCREM 2; hCREM-2; hCREM2; ICER; Inducible cAMP early repressor; Inducible cAMP early repressor ICER; MGC111110; MGC17881; MGC41893;
抗原和靶标
Expressed in testes (round spermatids) (at protein level). Isoform 14 is the major activator form in testes.
- Q03060 CREM_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTMETVESQHDGSITASLTESKSAHVQTQTGQNSIPALAQVSVAGSGTRRGSPAVTLVQLPSGQTIHVQGVIQTPQPWVIQSSEIHTVQVAAIAETDESAESEGVIDSHKRREILSRRPSYRKILNELSSDVPGVPKIEEERSEEEGTPPSIATMAVPTSIYQTSTGQYIAIAQGGTIQISNPGSDGVQGLQALTMTNSGAPPPGATIVQYAAQSADGTQQFFVPGSQVVVQDEETELAPSHMAAATGDMPTYQIRAPTAALPQGVVMAASPGSLHSPQQLAEEATRKRELRLMKNREAAKECRRRKKEYVKCLESRVAVLEVQNKKLIEELETLKDICSPKTDY
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q03060 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S42 | Phosphorylation | Uniprot | |
| S108 | Phosphorylation | Uniprot | |
| S116 | Phosphorylation | Uniprot | |
| S120 | Phosphorylation | Uniprot | |
| Y121 | Phosphorylation | Uniprot | |
| S129 | Phosphorylation | Uniprot | |
| T148 | Phosphorylation | Uniprot | |
| T259 | Phosphorylation | Uniprot | |
| S271 | Phosphorylation | Uniprot | |
| S274 | Phosphorylation | Uniprot | |
| S277 | Phosphorylation | Uniprot | |
| T286 | Phosphorylation | Uniprot |
研究背景
Transcriptional regulator that binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. Isoforms are either transcriptional activators or repressors. Plays a role in spermatogenesis and is involved in spermatid maturation.
May play a role in the regulation of the circadian clock: acts as a transcriptional repressor of the core circadian component PER1 by directly binding to cAMP response elements in its promoter.
Isoform 9 is ubiquitinated by CDC34 and RAD6B in order to be degraded by the proteasome.
Stimulated by phosphorylation. Phosphorylated on Ser-116 by TSSK4 in vitro.
Nucleus.
Cytoplasm. Nucleus.
Expressed in testes (round spermatids) (at protein level). Isoform 14 is the major activator form in testes.
Binds DNA as a dimer. Interacts with FHL5 (By similarity). Interacts with CDC34. May interact with TSSK4 (By similarity).
Belongs to the bZIP family.
研究领域
· Human Diseases > Infectious diseases: Viral > HTLV-I infection.
· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes. (View pathway)
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