(1)
(5)
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF7453, RRID:AB_2839390.
展开/折叠
AI843960; AOS3; CBF 1; CBF-1; CBF1; csl; IGKJRB; IGKJRB1; J kappa recombination signal binding protein; J kappa-recombination signal-binding protein; KBF2; NY REN 30 antigen; RBP J; RBP J kappa; RBP JK; RBP-J; RBP-J kappa; RBP-JK; Rbpj; RBPJK; RBPSUH; recombination signal binding protein for immunoglobulin kappa J region; Recombining binding protein suppressor of hairless; Renal carcinoma antigen NY-REN-30; SUH; SUH_HUMAN;
抗原和靶标
- Q06330 SUH_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDHTEGSPAEEPPAHAPSPGKFGERPPPKRLTREAMRNYLKERGDQTVLILHAKVAQKSYGNEKRFFCPPPCVYLMGSGWKKKKEQMERDGCSEQESQPCAFIGIGNSDQEMQQLNLEGKNYCTAKTLYISDSDKRKHFMLSVKMFYGNSDDIGVFLSKRIKVISKPSKKKQSLKNADLCIASGTKVALFNRLRSQTVSTRYLHVEGGNFHASSQQWGAFFIHLLDDDESEGEEFTVRDGYIHYGQTVKLVCSVTGMALPRLIIRKVDKQTALLDADDPVSQLHKCAFYLKDTERMYLCLSQERIIQFQATPCPKEPNKEMINDGASWTIISTDKAEYTFYEGMGPVLAPVTPVPVVESLQLNGGGDVAMLELTGQNFTPNLRVWFGDVEAETMYRCGESMLCVVPDISAFREGWRWVRQPVQVPVTLVRNDGIIYSTSLTFTYTPEPGPRPHCSAAGAILRANSSQVPPNESNTNSEGSYTNASTNSTSVTSSTATVVS
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q06330 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S7 | Phosphorylation | Uniprot | |
| S18 | Phosphorylation | Uniprot | |
| K58 | Ubiquitination | Uniprot | |
| Y122 | Phosphorylation | Uniprot | |
| T124 | Phosphorylation | Uniprot | |
| K126 | Acetylation | Uniprot | |
| K126 | Ubiquitination | Uniprot | |
| Y129 | Phosphorylation | Uniprot | |
| S131 | Phosphorylation | Uniprot | |
| S133 | Phosphorylation | Uniprot | |
| K135 | Ubiquitination | Uniprot | |
| K175 | Ubiquitination | Uniprot | |
| S195 | Phosphorylation | Uniprot | |
| T197 | Phosphorylation | Uniprot | |
| S199 | Phosphorylation | Uniprot | |
| T200 | Phosphorylation | Uniprot | |
| S230 | Phosphorylation | Uniprot | |
| T255 | Phosphorylation | Uniprot | |
| K266 | Acetylation | Uniprot | |
| K269 | Ubiquitination | Uniprot | |
| K285 | Ubiquitination | Uniprot | |
| K291 | Ubiquitination | Uniprot | |
| K315 | Ubiquitination | Uniprot | |
| T339 | Phosphorylation | Q16539 (MAPK14) | Uniprot |
研究背景
Transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations. Acts as a transcriptional repressor when it is not associated with Notch proteins. When associated with some NICD product of Notch proteins (Notch intracellular domain), it acts as a transcriptional activator that activates transcription of Notch target genes. Probably represses or activates transcription via the recruitment of chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins, respectively. Specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence. Binds specifically to methylated DNA. Binds to the oxygen responsive element of COX4I2 and activates its transcription under hypoxia conditions (4% oxygen). Negatively regulates the phagocyte oxidative burst in response to bacterial infection by repressing transcription of NADPH oxidase subunits (By similarity).
Nucleus. Cytoplasm.
Note: Mainly nuclear, upon interaction with RITA/C12orf52, translocates to the cytoplasm, down-regulating the Notch signaling pathway.
Interacts with activated NOTCH1, NOTCH2 or NOTCH3. Interacts with MINT/SHARP. This interaction may mediate the recruitment of large corepressor complexes containing proteins such as HDAC1, HDAC2, NCOR2, SAP30, FHL1/KYOT2 and CIR1. Interacts with EP300, MAML1 and PTF1A. Interacts with Epstein-Barr virus EBNA2, EBNA3, EBNA4 and EBNA6. Interacts with RITA1/C12orf52, leading to nuclear export, prevent the interaction between RBPJ and NICD product and subsequent down-regulation of the Notch signaling pathway. Interacts with SNW1. Interacts with CHCHD2 and CXXC5. Interacts with BEND6 (via BEN domain). Interacts with NKAPL (By similarity). Interacts with ZMIZ1. Interacts with RBM15 (By similarity).
Belongs to the Su(H) family.
研究领域
· Environmental Information Processing > Signal transduction > Notch signaling pathway. (View pathway)
· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.
· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.
· Human Diseases > Cancers: Overview > Viral carcinogenesis.
· Organismal Systems > Immune system > Th1 and Th2 cell differentiation. (View pathway)
文献引用
Application: WB Species: Rat Sample: spinal cord tissues
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