产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF7473, RRID:AB_2839410.
展开/折叠
Adenosinetriphosphatase; AT1B1_HUMAN; ATP 1B; ATP1B; ATP1B1; ATPase Na+/K+ transporting beta 1 polypeptide; Atpb 1; ATPBS; Beta 1 subunit of Na(+) K(+) ATPase; Na K ATPase beta 1 polypeptide; Na+/K+ ATPase beta 1 subunit; Sodium/potassium dependent ATPase beta 1 subunit; Sodium/potassium transporting ATPase beta 1 chain; Sodium/potassium transporting ATPase subunit beta 1; Sodium/potassium-dependent ATPase subunit beta-1; Sodium/potassium-transporting ATPase subunit beta-1;
抗原和靶标
- P05026 AT1B1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MARGKAKEEGSWKKFIWNSEKKEFLGRTGGSWFKILLFYVIFYGCLAGIFIGTIQVMLLTISEFKPTYQDRVAPPGLTQIPQIQKTEISFRPNDPKSYEAYVLNIVRFLEKYKDSAQRDDMIFEDCGDVPSEPKERGDFNHERGERKVCRFKLEWLGNCSGLNDETYGYKEGKPCIIIKLNRVLGFKPKPPKNESLETYPVMKYNPNVLPVQCTGKRDEDKDKVGNVEYFGLGNSPGFPLQYYPYYGKLLQPKYLQPLLAVQFTNLTMDTEIRIECKAYGENIGYSEKDRFQGRFDVKIEVKS
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P05026 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K13 | Ubiquitination | Uniprot | |
| K14 | Ubiquitination | Uniprot | |
| K21 | Ubiquitination | Uniprot | |
| K85 | Ubiquitination | Uniprot | |
| K96 | Ubiquitination | Uniprot | |
| Y101 | Phosphorylation | Uniprot | |
| K113 | Ubiquitination | Uniprot | |
| R118 | Methylation | Uniprot | |
| K134 | Ubiquitination | Uniprot | |
| K147 | Ubiquitination | Uniprot | |
| K152 | Ubiquitination | Uniprot | |
| N158 | N-Glycosylation | Uniprot | |
| K170 | Ubiquitination | Uniprot | |
| K173 | Ubiquitination | Uniprot | |
| K179 | Ubiquitination | Uniprot | |
| K187 | Ubiquitination | Uniprot | |
| N193 | N-Glycosylation | Uniprot | |
| K216 | Ubiquitination | Uniprot | |
| K221 | Ubiquitination | Uniprot | |
| K223 | Ubiquitination | Uniprot | |
| Y229 | Phosphorylation | Uniprot | |
| S235 | Phosphorylation | Uniprot | |
| K248 | Ubiquitination | Uniprot | |
| Y254 | Phosphorylation | Uniprot | |
| N265 | N-Glycosylation | Uniprot | |
| K277 | Ubiquitination | Uniprot | |
| Y285 | Phosphorylation | Uniprot | |
| K288 | Ubiquitination | Uniprot |
研究背景
This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane.
Involved in cell adhesion and establishing epithelial cell polarity.
Glutathionylated.
Cell membrane>Single-pass type II membrane protein. Cell membrane>Sarcolemma.
Note: Colocalizes with OBSCN at the intercalated disk and sarcolemma in cardiomyocytes. Localizes in long striations at the level of Z and M lines.
Found in most tissues.
The sodium/potassium-transporting ATPase is composed of a catalytic alpha subunit, an auxiliary non-catalytic beta subunit and an additional regulatory subunit. Interacts with regulatory subunit FXYD1 (By similarity). Interacts with regulatory subunit FXYD3 (By similarity). Interacts with NKAIN1, NKAIN2 and NKAIN4 (By similarity). Interacts with MLC1. Part of a complex containing MLC1, TRPV4, AQP4 and HEPACAM. Interacts with KIRREL3. Interacts with OBSCN (via protein kinase domain 1) (By similarity).
The C-terminal lobe folds into an immunoglobulin-like domain and mediates cell adhesion properties.
Belongs to the X(+)/potassium ATPases subunit beta family.
研究领域
· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > cAMP signaling pathway. (View pathway)
· Organismal Systems > Circulatory system > Cardiac muscle contraction. (View pathway)
· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes. (View pathway)
· Organismal Systems > Endocrine system > Insulin secretion. (View pathway)
· Organismal Systems > Endocrine system > Thyroid hormone synthesis.
· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway. (View pathway)
· Organismal Systems > Endocrine system > Aldosterone synthesis and secretion.
· Organismal Systems > Excretory system > Aldosterone-regulated sodium reabsorption.
· Organismal Systems > Excretory system > Endocrine and other factor-regulated calcium reabsorption.
· Organismal Systems > Excretory system > Proximal tubule bicarbonate reclamation.
· Organismal Systems > Digestive system > Salivary secretion.
· Organismal Systems > Digestive system > Gastric acid secretion.
· Organismal Systems > Digestive system > Pancreatic secretion.
· Organismal Systems > Digestive system > Carbohydrate digestion and absorption.
· Organismal Systems > Digestive system > Protein digestion and absorption.
· Organismal Systems > Digestive system > Mineral absorption.
限制条款
产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。
产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。
Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。产品仅供科学研究使用。不用于诊断和治疗。
产品未经授权不得转售。
Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.