Tubulin gamma Antibody | Affinity Biosciences LTD|亲科生物官网

WB
IF/ICC
Cites

1/5

Western blot analysis of Tubulin gamma expression in Rat brain lysate.

Western blot analysis of extracts from MCF7, using Tubulin gamma Antibody.

AF7012 staining Hela cells by IF/ICC.

AF7012 staining MCF-7 cells by ICC/IF.

Figure 4 Effects of FV on liver lipogenesis-related markers in the mice fed with HFD.

产品:	Tubulin gamma 抗体
货号:	AF7012
描述:	Rabbit polyclonal antibody to Tubulin gamma
应用:	WB IHC IF/ICC
反应:	Human, Mouse, Rat
预测:	Zebrafish, Rabbit, Xenopus
分子量:	51kDa; 51kD(Calculated).
蛋白号:	P23258
RRID:	AB_2839419

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阻断肽(封闭肽)是特异性结合目标抗体和阻断抗体结合的多肽。这些多肽包含抗体识别的抗原表位。与阻断肽结合的抗体不再与靶蛋白上的表位结合。例如，在免疫印迹(WB)和免疫组化(IHC)中，通过比较被阻断抗体和未阻断抗体的染色，可以看到哪种染色是特异性的。

规格	价格	库存
50ul	RMB¥ 900	现货
100ul	RMB¥ 1500	现货
200ul	RMB¥ 2000	现货

货期: 当天发货

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实验方案

产品描述

来源:

Rabbit

应用:

WB 1:000-1:5000, IHC 1:50-1:200, IF/ICC 1:200-500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:

Human,Mouse,Rat

预测:

Zebrafish(87%), Rabbit(87%), Xenopus(80%)

克隆:

Polyclonal

特异性:

Tubulin gamma Antibody detects endogenous levels of total Tubulin gamma.

RRID:

AB_2839419
引用格式: Affinity Biosciences Cat# AF7012, RRID:AB_2839419.

偶联:

Unconjugated.

纯化:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

别名:

展开/折叠

TUBG1, Gamma-1-tubulin, GCP-1, Gamma Tubulin, TUBGCP1, Tubulin gamma chain, TUBG, Tubulin, gamma polypeptide, Tubulin gamma-1 chain, Tubulin, gamma 1

抗原和靶标

免疫原:

A synthesized peptide derived from human Tubulin gamma.

Uniprot:

P23258(TBG1_HUMAN) >>Visit HPA database.

基因/基因ID:

TUBG1(7283)

描述:

TUBG1 Tubulin is the major constituent of microtubules. Gamma tubulin is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta tubulin minus-end nucleation, centrosome duplication and spindle formation. Interacts with GCP2 and GCP3. Interacts with B9D2. Interacts with CDK5RAP2;

序列:

P23258 TBG1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MPREIITLQLGQCGNQIGFEFWKQLCAEHGISPEGIVEEFATEGTDRKDVFFYQADDEHYIPRAVLLDLEPRVIHSILNSPYAKLYNPENIYLSEHGGGAGNNWASGFSQGEKIHEDIFDIIDREADGSDSLEGFVLCHSIAGGTGSGLGSYLLERLNDRYPKKLVQTYSVFPNQDEMSDVVVQPYNSLLTLKRLTQNADCVVVLDNTALNRIATDRLHIQNPSFSQINQLVSTIMSASTTTLRYPGYMNNDLIGLIASLIPTPRLHFLMTGYTPLTTDQSVASVRKTTVLDVMRRLLQPKNVMVSTGRDRQTNHCYIAILNIIQGEVDPTQVHKSLQRIRERKLANFIPWGPASIQVALSRKSPYLPSAHRVSGLMMANHTSISSLFERTCRQYDKLRKREAFLEQFRKEDMFKDNFDEMDTSREIVQQLIDEYHAATRPDYISWGTQEQ

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species

Results

Score

Rabbit

Zebrafish

Xenopus

Pig

Horse

Bovine

Sheep

Dog

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P23258 作为底物

P23258

Site	PTM Type	Enzyme	Source
S32	Phosphorylation		Uniprot
K48	Ubiquitination		Uniprot
S80	Phosphorylation	P24941 (CDK2)	Uniprot
K84	Ubiquitination		Uniprot
K113	Ubiquitination		Uniprot
S129	Phosphorylation		Uniprot
S131	Phosphorylation	Q8TDC3-2 (BRSK1)	Uniprot
K164	Ubiquitination		Uniprot
K193	Ubiquitination		Uniprot
S284	Phosphorylation		Uniprot
K287	Ubiquitination		Uniprot
R295	Methylation		Uniprot
K301	Ubiquitination		Uniprot
S306	Phosphorylation		Uniprot
T307	Phosphorylation		Uniprot
K335	Ubiquitination		Uniprot
S336	Phosphorylation		Uniprot
K344	Ubiquitination		Uniprot
K363	Ubiquitination		Uniprot
S364	Phosphorylation		Uniprot
S385	Phosphorylation	Q8TDC3 (BRSK1)	Uniprot
K397	Acetylation		Uniprot
K400	Acetylation		Uniprot
K400	Ubiquitination		Uniprot
K410	Ubiquitination		Uniprot
K415	Ubiquitination		Uniprot
T423	Phosphorylation		Uniprot
S424	Phosphorylation		Uniprot

研究背景

P23258_TBG1_HUMAN

功能:

Tubulin is the major constituent of microtubules. The gamma chain is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta chain minus-end nucleation, centrosome duplication and spindle formation.

翻译修饰:

Phosphorylation at Ser-131 by BRSK1 regulates centrosome duplication, possibly by mediating relocation of gamma-tubulin and its associated proteins from the cytoplasm to the centrosome.

细胞定位:

Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.

亚基结构:

Interacts with TUBGCP2 and TUBGCP3. Interacts with B9D2 (By similarity). Interacts with CDK5RAP2; the interaction is leading to centrosomal localization of TUBG1 and CDK5RAP2. Interacts with PIFO. Interacts with SAS6 and NUP62 at the centrosome.

蛋白家族:

Belongs to the tubulin family.

研究领域

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

文献引用

1). Ficus hirta Vahl. Ameliorates Nonalcoholic Fatty Liver Disease through Regulating Lipid Metabolism and Gut Microbiota. Oxidative Medicine and Cellular Longevity (PubMed: 35592528)

Application: WB Species: Mouse Sample:

Figure 4 Effects of FV on liver lipogenesis-related markers in the mice fed with HFD. (a–h) The relative mRNA expression levels of Acaca, Cd36, Cpt1α, Srebp-1, Hmgcr, Pparα, Pparγ, and Scd1 were determined by qRT-PCR. (i) The lipid metabolism relevant protein levels of SREBP-1, ACOX1, CD36, CPT1α, and HMGCR were analyzed by western blotting, and the relative ratios were calculated. (j–l) Serum IL-6, IL-1β, and TNF-α were identified by ELISA kits. (m, n) The relative mRNA expression levels of IL-1β, TNF-α, and Ccl5 were identified by qRT-PCR. (p) The proinflammatory factor protein levels of IL-1β, IL-6, and TNF-α were analyzed by western blotting, and their relative ratios were calculated. The densitometry was obtained by averaging repeated experiments results, normalized to GAPDH. The data are expressed as the mean ± SD, n = 3. #P < 0.05 means there is significant difference between the NFD group and the HFD group. ∗P < 0.05 means the difference between the HFD group and the FV-L group or the FV-H group is significant.

2). The antioxidant Glycitin protects against intervertebral disc degeneration through antagonizing inflammation and oxidative stress in nucleus pulposus cells. Aging (Albany NY) (PubMed: 38019477)

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Tubulin gamma Antibody - #AF7012

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