Tubulin gamma Antibody - #AF7012

Fig. 3. The effect of FV on MCD-induced hepatic fibrosis in mice. (A)H&E staining of liver tissues in different groups; (B)Masson image staining of liver tissues in different groups; (C)Sirus Red staining of liver tissues in different groups; (D)liver index; (E)quantification of the collagen fiber area (%) using Image J; (F)quantification of the collagen fiber area (%), using Image J; (G-J)serum ALT & AST, liver TC & TG (n = 6); (K-M)The fibrosis protein levels of α-SMA, collagen Ⅰ were detected by western blotting and the relative ratios were calculated (n = 3); (N–R) The relative mRNA expression levels of α-SMA, COL1A1, TGF-β1, TNF-α and IL-1β were determined by qRT -PCR (n = 3). NC, normal group; MCD, MCD diet group; FV-L, FV-L group; FV-H, FV-L group. The data are expressed in the format of mean ± SD. *p < 0.05, **p < 0.01, and ***p < 0.001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Figure 4 Effects of FV on liver lipogenesis-related markers in the mice fed with HFD. (a–h) The relative mRNA expression levels of Acaca, Cd36, Cpt1α, Srebp-1, Hmgcr, Pparα, Pparγ, and Scd1 were determined by qRT-PCR. (i) The lipid metabolism relevant protein levels of SREBP-1, ACOX1, CD36, CPT1α, and HMGCR were analyzed by western blotting, and the relative ratios were calculated. (j–l) Serum IL-6, IL-1β, and TNF-α were identified by ELISA kits. (m, n) The relative mRNA expression levels of IL-1β, TNF-α, and Ccl5 were identified by qRT-PCR. (p) The proinflammatory factor protein levels of IL-1β, IL-6, and TNF-α were analyzed by western blotting, and their relative ratios were calculated. The densitometry was obtained by averaging repeated experiments results, normalized to GAPDH. The data are expressed as the mean ± SD, n = 3. #P < 0.05 means there is significant difference between the NFD group and the HFD group. ∗P < 0.05 means the difference between the HFD group and the FV-L group or the FV-H group is significant.

Figure 1 Glycitin alleviated the TNF-α-induced inflammatory response. (A, B) Transcriptional and (C) protein levels of iNOS and COX-2 in human primary nucleus pulposus cells as determined by RT-PCR and Western blotting. (D, E) Quantitative analysis of immunoblotting in (C), assayed by ImageJ program. (F, G) IF staining of COX-2, fluorescence intensity analysis was performed using ImageJ program. Scale bar: 100 μm. The values represent the mean ± SD of three independent experiments. *p < 0.05 vs. control group.