beta Actin Antibody - #AF7018
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产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF7018, RRID:AB_2839420.
展开/折叠
ACTB; Actin; cytoplasmic 1; Beta-actin; Beta actin; BRWS1; β actin;b actin; Actin beta; Beta cytoskeletal actin; PS1TP5-binding protein 1; PS1TP5BP1;
抗原和靶标
A synthesized peptide derived from human Beta actin.
- P60709 ACTB_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF
种属预测
score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P60709 作为底物
Site | PTM Type | Enzyme | Source |
---|---|---|---|
M1 | Acetylation | Uniprot | |
D2 | Acetylation | Uniprot | |
S14 | Phosphorylation | Uniprot | |
C17 | S-Nitrosylation | Uniprot | |
K18 | Methylation | Uniprot | |
K18 | Ubiquitination | Uniprot | |
S33 | Phosphorylation | Uniprot | |
K50 | Acetylation | Uniprot | |
K50 | Methylation | Uniprot | |
K50 | Ubiquitination | Uniprot | |
S52 | Phosphorylation | Uniprot | |
Y53 | Phosphorylation | Uniprot | |
S60 | Phosphorylation | Uniprot | |
K61 | Acetylation | Uniprot | |
K61 | Sumoylation | Uniprot | |
K61 | Ubiquitination | Uniprot | |
T66 | Phosphorylation | Uniprot | |
K68 | Methylation | Uniprot | |
K68 | Sumoylation | Uniprot | |
Y69 | Phosphorylation | Uniprot | |
H73 | Methylation | Uniprot | |
T77 | Phosphorylation | Uniprot | |
K84 | Methylation | Uniprot | |
K84 | Sumoylation | Uniprot | |
K84 | Ubiquitination | Uniprot | |
T89 | Phosphorylation | Uniprot | |
Y91 | Phosphorylation | Uniprot | |
T106 | Phosphorylation | Uniprot | |
K113 | Acetylation | Uniprot | |
K113 | Sumoylation | Uniprot | |
K113 | Ubiquitination | Uniprot | |
T120 | Phosphorylation | Uniprot | |
Y143 | Phosphorylation | Uniprot | |
S155 | Phosphorylation | Uniprot | |
T160 | Phosphorylation | Uniprot | |
T162 | Phosphorylation | Uniprot | |
Y166 | Phosphorylation | Uniprot | |
Y169 | Phosphorylation | Uniprot | |
T186 | Phosphorylation | Uniprot | |
Y188 | Phosphorylation | Uniprot | |
K191 | Acetylation | Uniprot | |
K191 | Methylation | Uniprot | |
K191 | Ubiquitination | Uniprot | |
T194 | Phosphorylation | Uniprot | |
Y198 | Phosphorylation | Uniprot | |
S199 | Phosphorylation | Uniprot | |
T201 | Phosphorylation | Uniprot | |
T202 | Phosphorylation | Uniprot | |
T203 | Phosphorylation | Uniprot | |
K213 | Acetylation | Uniprot | |
K213 | Ubiquitination | Uniprot | |
K215 | Ubiquitination | Uniprot | |
C217 | S-Nitrosylation | Uniprot | |
Y218 | Phosphorylation | Uniprot | |
T229 | Phosphorylation | Uniprot | |
S233 | Phosphorylation | Uniprot | |
S235 | Phosphorylation | Uniprot | |
K238 | Ubiquitination | Uniprot | |
S239 | Phosphorylation | Uniprot | |
Y240 | Phosphorylation | Uniprot | |
T249 | Phosphorylation | Uniprot | |
C257 | S-Nitrosylation | Uniprot | |
S265 | Phosphorylation | Uniprot | |
S271 | Phosphorylation | Uniprot | |
C272 | S-Nitrosylation | Uniprot | |
K284 | Sumoylation | Uniprot | |
K284 | Ubiquitination | Uniprot | |
C285 | S-Nitrosylation | Uniprot | |
K291 | Sumoylation | Uniprot | |
K291 | Ubiquitination | Uniprot | |
Y294 | Phosphorylation | Uniprot | |
T297 | Phosphorylation | Uniprot | |
S300 | Phosphorylation | Uniprot | |
T303 | Phosphorylation | Uniprot | |
T304 | Phosphorylation | Uniprot | |
Y306 | Phosphorylation | Uniprot | |
K315 | Acetylation | Uniprot | |
K315 | Sumoylation | Uniprot | |
K315 | Ubiquitination | Uniprot | |
T318 | Phosphorylation | Uniprot | |
S323 | Phosphorylation | Uniprot | |
T324 | Phosphorylation | Uniprot | |
K326 | Acetylation | Uniprot | |
K326 | Methylation | Uniprot | |
K326 | Sumoylation | Uniprot | |
K326 | Ubiquitination | Uniprot | |
K328 | Acetylation | Uniprot | |
K328 | Sumoylation | Uniprot | |
K328 | Ubiquitination | Uniprot | |
K336 | Sumoylation | Uniprot | |
S348 | Phosphorylation | Uniprot | |
K359 | Ubiquitination | Uniprot | |
Y362 | Phosphorylation | Uniprot | |
S365 | Phosphorylation | Uniprot | |
S368 | Phosphorylation | Uniprot | |
K373 | Ubiquitination | Uniprot | |
C374 | S-Nitrosylation | Uniprot |
研究背景
Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells. Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction. In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA.
ISGylated.
Oxidation of Met-44 and Met-47 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization.
Monomethylation at Lys-84 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration.
Methylated at His-73 by SETD3. Methylation at His-73 is required for smooth muscle contraction of the laboring uterus during delivery (By similarity).
N-terminal acetylation by NAA80 affects actin filament depolymerization and elongation, including elongation driven by formins. In contrast, filament nucleation by the Arp2/3 complex is not affected.
(Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-50 of one monomer and Glu-270 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding. The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners.
Cytoplasm>Cytoskeleton. Nucleus.
Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs. Component of the BAF complex, which includes at least actin (ACTB), ARID1A, ARID1B/BAF250, SMARCA2, SMARCA4/BRG1, ACTL6A/BAF53, ACTL6B/BAF53B, SMARCE1/BAF57 SMARCC1/BAF155, SMARCC2/BAF170, SMARCB1/SNF5/INI1, and one or more of SMARCD1/BAF60A, SMARCD2/BAF60B, or SMARCD3/BAF60C. In muscle cells, the BAF complex also contains DPF3. Found in a complex with XPO6, Ran, ACTB and PFN1. Interacts with XPO6 and EMD. Interacts with ERBB2. Interacts with GCSAM. Interacts with TBC1D21 (By similarity). Interacts with CPNE1 (via VWFA domain) and CPNE4 (via VWFA domain) (By similarity). Interacts with DHX9 (via C-terminus); this interaction is direct and mediates the attachment to nuclear ribonucleoprotein complexes. Interacts with FAM107A.
Belongs to the actin family.
研究领域
· Cellular Processes > Transport and catabolism > Phagosome. (View pathway)
· Cellular Processes > Cell growth and death > Apoptosis. (View pathway)
· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)
· Cellular Processes > Cellular community - eukaryotes > Adherens junction. (View pathway)
· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)
· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)
· Environmental Information Processing > Signal transduction > Rap1 signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Hippo signaling pathway. (View pathway)
· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.
· Human Diseases > Infectious diseases: Bacterial > Vibrio cholerae infection.
· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.
· Human Diseases > Infectious diseases: Bacterial > Shigellosis.
· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.
· Human Diseases > Infectious diseases: Viral > Influenza A.
· Human Diseases > Cancers: Overview > Proteoglycans in cancer.
· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma. (View pathway)
· Human Diseases > Cardiovascular diseases > Hypertrophic cardiomyopathy (HCM).
· Human Diseases > Cardiovascular diseases > Arrhythmogenic right ventricular cardiomyopathy (ARVC).
· Human Diseases > Cardiovascular diseases > Dilated cardiomyopathy (DCM).
· Human Diseases > Cardiovascular diseases > Viral myocarditis.
· Organismal Systems > Immune system > Platelet activation. (View pathway)
· Organismal Systems > Immune system > Leukocyte transendothelial migration. (View pathway)
· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway. (View pathway)
· Organismal Systems > Endocrine system > Oxytocin signaling pathway.
· Organismal Systems > Digestive system > Gastric acid secretion.
文献引用
Application: WB Species: mouse Sample: cells
Application: WB Species: mouse Sample: liver
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