MCF2L Antibody - #DF2202

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产品: MCF2L 抗体
货号: DF2202
描述: Rabbit polyclonal antibody to MCF2L
应用: WB IHC
反应: Human, Rat
分子量: 128kDa; 128kD(Calculated).
蛋白号: O15068
RRID: AB_2839433

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Rat
克隆:
Polyclonal
特异性:
MCF2L Antibody detects endogenous levels of total MCF2L.
RRID:
AB_2839433
引用格式: Affinity Biosciences Cat# DF2202, RRID:AB_2839433.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

ARHGEF 14; DBL''s big sister; DBS; FLJ12122; Guanine nucleotide exchange factor DBS; KIAA0362; MCF 2L; MCF.2 cell line derived transforming sequence like; MCF.2 transforming sequence-like; MCF2 like protein; MCF2 transforming sequence like protein; MCF2-transforming sequence-like protein; Mcf2l; MCF2L_HUMAN; OST; OST oncogene;

抗原和靶标

免疫原:
Uniprot:

O15068(MCF2L_HUMAN) >>Visit HPA database.

基因/基因ID:
MCF2L(23263)
描述:
Guanine nucleotide exchange factor that potentially links pathways that signal through RAC1, RHOA and CDC42. Catalyzes guanine nucleotide exchange on RHOA and CDC42 and interacts specifically with the GTP-bound form of RAC1, suggesting that it functions as an effector of RAC1. May also participate in axonal transport in the brain. Becomes activated and highly tumorigenic by truncation of the N-terminus (By similarity). Isoform 5 activates CDC42.
序列:
MFDCWRFILCKRPGSNSYSSPQRPNEAKKEETDHQIDVSDVIRLVQDTPEATAMATDEIMHQDIVPLCAADIQDQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRRDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDDFKMKVPVIMLSSVPDLHGYIDKSQLTEDLGGTLDYCHSRWLCQRTAIESFALMVKQTAQMLQSFGTELAETELPNDVQSTSSVLCAHTEKKDKAKEDLRLALKEGHSVLESLRELQAEGSEPSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKAILDAASQKIATFTDIGNSLAHVEHLLRDLASFEEKSGVAVERARALSLDGEQLIGNKHYAVDSIRPKCQELRHLCDQFSAEIARRRGLLSKSLELHRRLETSMKWCDEGIYLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNAIYKEYESILNQDLMEHVRKVFQKQASMEEVFHRRQASLKKLAARQTRPVQPVAPRPEALAKSPCPSPGIRRGSENSSSEGGALRRGPYRRAKSEMSESRQGRGSAGEEEESLAILRRHVMSELLDTERAYVEELLCVLEGYAAEMDNPLMAHLLSTGLHNKKDVLFGNMEEIYHFHNRIFLRELENYTDCPELVGRCFLERMEDFQIYEKYCQNKPRSESLWRQCSDCPFFQECQRKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSRNCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKRGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMAAVGITENVKGDAKKFEIWYNAREEVYIVQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSQSLPLPAPTSTSPSRGNSRNIKKLEERKTDPLSLEGYVSSAPLTKPPEKGKGWSKTSHSLEAPEDDGGWSSAEEQINSSDAEEDGGLGPKKLVPGKYTVVADHEKGGPDALRVRSGDVVELVQEGDEGLWYVRDPTTGKEGWVPASSLSVRLGPSGSAQCLSSSGKAHVPRAHP

翻译修饰 - O15068 作为底物

Site PTM Type Enzyme
T96 Phosphorylation
S177 Phosphorylation
K182 Ubiquitination
Y222 Phosphorylation
K364 Ubiquitination
S371 Phosphorylation
S396 Phosphorylation
S412 Phosphorylation
K456 Ubiquitination
S578 Phosphorylation
S582 Phosphorylation
S589 Phosphorylation
S592 Phosphorylation
S594 Phosphorylation
K608 Acetylation
S609 Phosphorylation
S620 Phosphorylation
Y784 Phosphorylation
S785 Phosphorylation
S893 Phosphorylation
T902 Phosphorylation
S965 Phosphorylation
T972 Phosphorylation
S975 Phosphorylation
T992 Phosphorylation
Y1000 Phosphorylation
T1007 Phosphorylation
S1033 Phosphorylation
S1034 Phosphorylation

研究背景

功能:

Guanine nucleotide exchange factor that catalyzes guanine nucleotide exchange on RHOA and CDC42, and thereby contributes to the regulation of RHOA and CDC42 signaling pathways (By similarity). Seems to lack activity with RAC1. Becomes activated and highly tumorigenic by truncation of the N-terminus (By similarity). Isoform 5 activates CDC42.

Does not catalyze guanine nucleotide exchange on CDC42.

细胞定位:

Cytoplasm. Cell membrane>Peripheral membrane protein>Cytoplasmic side.

Cytoplasm. Endomembrane system.
Note: Interaction with membranes enriched in phosphoinositides is mediated by the CRAL-TRIO domain.

Cytoplasm. Cell membrane>Peripheral membrane protein>Cytoplasmic side.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Interacts with GTP-bound RAC1 (By similarity). Interacts with CDC42. Interacts with RHOA. Interacts with CCPG1, which results in specific inhibition of its exchange activity toward RHOA, but does not affect its activity on CDC42 (By similarity).

蛋白家族:

The CRAL-TRIO domain mediates interaction with various inositol phospholipids, such as phosphatidylinositol 3-phosphate (PI3P), phosphatidylinositol 4-phosphate (PI4P) and phosphatidylinositol 5-phosphate (PI5P).

The DH domain is involved in interaction with CCPG1.

Belongs to the MCF2 family.

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