产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF2211, RRID:AB_2839442.
展开/折叠
CI 42KD; CI-42k; Complex I-42kD; MGC5103; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 10; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 10 42kDa; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial complex I 42kDa subunit; NADH ubiquinone oxidoreductase 42 kDa subunit mitochondrial precursor; NADH-ubiquinone oxidoreductase 42 kDa subunit; ndufa10l;
抗原和靶标
- O95299 NDUAA_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MALRLLKLAATSASARVVAAGAQRVRGIHSSVQCKLRYGMWHFLLGDKASKRLTERSRVITVDGNICTGKGKLAKEIAEKLGFKHFPEAGIHYPDSTTGDGKPLATDYNGNCSLEKFYDDPRSNDGNSYRLQSWLYSSRLLQYSDALEHLLTTGQGVVLERSIFSDFVFLEAMYNQGFIRKQCVDHYNEVKSVTICDYLPPHLVIYIDVPVPEVQRRIQKKGDPHEMKITSAYLQDIENAYKKTFLPEMSEKCEVLQYSAREAQDSKKVVEDIEYLKFDKGPWLKQDNRTLYHLRLLVQDKFEVLNYTSIPIFLPEVTIGAHQTDRVLHQFRELPGRKYSPGYNTEVGDKWIWLK
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O95299 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K48 | Ubiquitination | Uniprot | |
| S57 | Phosphorylation | Uniprot | |
| T61 | Phosphorylation | Uniprot | |
| K80 | Acetylation | Uniprot | |
| K80 | Ubiquitination | Uniprot | |
| K84 | Ubiquitination | Uniprot | |
| K102 | Ubiquitination | Uniprot | |
| K181 | Ubiquitination | Uniprot | |
| K221 | Acetylation | Uniprot | |
| T230 | Phosphorylation | Uniprot | |
| S231 | Phosphorylation | Uniprot | |
| Y233 | Phosphorylation | Uniprot | |
| Y241 | Phosphorylation | Uniprot | |
| K242 | Acetylation | Uniprot | |
| K242 | Ubiquitination | Uniprot | |
| K243 | Acetylation | Uniprot | |
| K243 | Ubiquitination | Uniprot | |
| S250 | Phosphorylation | Uniprot | |
| K252 | Ubiquitination | Uniprot | |
| K267 | Acetylation | Uniprot | |
| K268 | Ubiquitination | Uniprot | |
| Y275 | Phosphorylation | Uniprot | |
| K277 | Ubiquitination | Uniprot | |
| K280 | Ubiquitination | Uniprot | |
| K285 | Ubiquitination | Uniprot | |
| K338 | Ubiquitination | Uniprot | |
| T345 | Phosphorylation | Uniprot | |
| K350 | Ubiquitination | Uniprot |
研究背景
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Phosphorylation at Ser-250 by PINK1 is required for the binding and/or reduction of the complex I substrate ubiquinone.
Mitochondrion matrix.
Complex I is composed of 45 different subunits. This a component of the hydrophobic protein fraction.
Belongs to the complex I NDUFA10 subunit family.
研究领域
· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).
· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.
· Human Diseases > Neurodegenerative diseases > Parkinson's disease.
· Human Diseases > Neurodegenerative diseases > Huntington's disease.
· Metabolism > Energy metabolism > Oxidative phosphorylation.
· Metabolism > Global and overview maps > Metabolic pathways.
· Organismal Systems > Nervous system > Retrograde endocannabinoid signaling. (View pathway)
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