产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF2234, RRID:AB_2839465.
展开/折叠
FLJ11159; OTTHUMP00000158911; OTTHUMP00000222868; RIO kinase 2 (yeast); RIO kinase 2; RIO2; RIOK2; RIOK2_HUMAN; Serine/threonine protein kinase RIO2; Serine/threonine-protein kinase rio2;
抗原和靶标
- Q9BVS4 RIOK2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGKVNVAKLRYMSRDDFRVLTAVEMGMKNHEIVPGSLIASIASLKHGGCNKVLRELVKHKLIAWERTKTVQGYRLTNAGYDYLALKTLSSRQVVESVGNQMGVGKESDIYIVANEEGQQFALKLHRLGRTSFRNLKNKRDYHKHRHNVSWLYLSRLSAMKEFAYMKALYERKFPVPKPIDYNRHAVVMELINGYPLCQIHHVEDPASVYDEAMELIVKLANHGLIHGDFNEFNLILDESDHITMIDFPQMVSTSHPNAEWYFDRDVKCIKDFFMKRFSYESELFPTFKDIRREDTLDVEVSASGYTKEMQADDELLHPLGPDDKNIETKEGSEFSFSDGEVAEKAEVYGSENESERNCLEESEGCYCRSSGDPEQIKEDSLSEESADARSFEMTEFNQALEEIKGQVVENNSVTEFSEEKNRTENYNRQDGQRVQGGVPAGSDEYEDECPHLIALSSLNREFRPFRDEENVGAMNQYRTRTLSITSSGSAVSCSTIPPELVKQKVKRQLTKQQKSAVRRRLQKGEANIFTKQRRENMQNIKSSLEAASFWGE
翻译修饰 - Q9BVS4 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K8 | Ubiquitination | Uniprot | |
| T21 | Phosphorylation | Uniprot | |
| K45 | Ubiquitination | Uniprot | |
| K51 | Ubiquitination | Uniprot | |
| K86 | Ubiquitination | Uniprot | |
| T130 | Phosphorylation | Uniprot | |
| S131 | Phosphorylation | Uniprot | |
| K160 | Ubiquitination | Uniprot | |
| Y181 | Phosphorylation | Uniprot | |
| K270 | Ubiquitination | Uniprot | |
| K275 | Ubiquitination | Uniprot | |
| K288 | Ubiquitination | Uniprot | |
| S332 | Phosphorylation | Uniprot | |
| S335 | Phosphorylation | P53350 (PLK1) | Uniprot |
| S337 | Phosphorylation | Uniprot | |
| Y348 | Phosphorylation | Uniprot | |
| S350 | Phosphorylation | Uniprot | |
| S354 | Phosphorylation | Uniprot | |
| S362 | Phosphorylation | Uniprot | |
| Y366 | Phosphorylation | Uniprot | |
| S369 | Phosphorylation | Uniprot | |
| S370 | Phosphorylation | Uniprot | |
| K377 | Ubiquitination | Uniprot | |
| S380 | Phosphorylation | P53350 (PLK1) | Uniprot |
| S382 | Phosphorylation | Uniprot | |
| S385 | Phosphorylation | Uniprot | |
| S390 | Phosphorylation | Uniprot | |
| S412 | Phosphorylation | Uniprot | |
| T414 | Phosphorylation | Uniprot | |
| S417 | Phosphorylation | Uniprot | |
| K420 | Ubiquitination | Uniprot | |
| Y426 | Phosphorylation | Uniprot | |
| S442 | Phosphorylation | Uniprot | |
| Y445 | Phosphorylation | Uniprot | |
| S457 | Phosphorylation | Uniprot | |
| T481 | Phosphorylation | Uniprot | |
| S483 | Phosphorylation | Uniprot | |
| T485 | Phosphorylation | Uniprot | |
| S486 | Phosphorylation | Uniprot | |
| S487 | Phosphorylation | Uniprot | |
| S489 | Phosphorylation | Uniprot | |
| T510 | Phosphorylation | Uniprot | |
| K523 | Ubiquitination | Uniprot | |
| K531 | Methylation | Uniprot | |
| K531 | Ubiquitination | Uniprot | |
| S548 | Phosphorylation | P53350 (PLK1) | Uniprot |
研究背景
Serine/threonine-protein kinase involved in the final steps of cytoplasmic maturation of the 40S ribosomal subunit. Involved in export of the 40S pre-ribosome particles (pre-40S) from the nucleus to the cytoplasm. Its kinase activity is required for the release of NOB1, PNO1 and LTV1 from the late pre-40S and the processing of 18S-E pre-rRNA to the mature 18S rRNA. Regulates the timing of the metaphase-anaphase transition during mitotic progression, and its phosphorylation, most likely by PLK1, regulates this function.
Autophosphorylated (in vitro). Phosphorylation at Ser-335, Ser-380, Ser-548 by PLK1 affects the timing of the metaphase-anaphase transition.
Cytoplasm.
Note: Exported out of the nucleus via its NES in a XPO1-dependent manner.
Associated with late 40S pre-ribosomal particles. Interacts with PLK1 (via its N-terminus).
Belongs to the protein kinase superfamily. RIO-type Ser/Thr kinase family.
研究领域
· Genetic Information Processing > Translation > Ribosome biogenesis in eukaryotes.
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