| 产品: | SF3A3 抗体 |
| 货号: | DF2246 |
| 描述: | Rabbit polyclonal antibody to SF3A3 |
| 应用: | WB |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 59 kDa; 59kD(Calculated). |
| 蛋白号: | Q12874 |
| RRID: | AB_2839477 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF2246, RRID:AB_2839477.
展开/折叠
SF3A3; Pre mRNA splicing factor SF3a (60kD); Pre mRNA splicing factor SF3a; PRP 9; PRP9; PRPF 9; PRPF9; SAP 61; SAP61; SF3a60; Spliceosome associated protein 61; Splicing factor 3a subunit 3 60kDa; Splicing factor 3a subunit 3;
抗原和靶标
- Q12874 SF3A3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
METILEQQRRYHEEKERLMDVMAKEMLTKKSTLRDQINSDHRTRAMQDRYMEVSGNLRDLYDDKDGLRKEELNAISGPNEFAEFYNRLKQIKEFHRKHPNEICVPMSVEFEELLKARENPSEEAQNLVEFTDEEGYGRYLDLHDCYLKYINLKASEKLDYITYLSIFDQLFDIPKERKNAEYKRYLEMLLEYLQDYTDRVKPLQDQNELFGKIQAEFEKKWENGTFPGWPKETSSALTHAGAHLDLSAFSSWEELASLGLDRLKSALLALGLKCGGTLEERAQRLFSTKGKSLESLDTSLFAKNPKSKGTKRDTERNKDIAFLEAQIYEYVEILGEQRHLTHENVQRKQARTGEEREEEEEEQISESESEDEENEIIYNPKNLPLGWDGKPIPYWLYKLHGLNINYNCEICGNYTYRGPKAFQRHFAEWRHAHGMRCLGIPNTAHFANVTQIEDAVSLWAKLKLQKASERWQPDTEEEYEDSSGNVVNKKTYEDLKRQGLL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q12874 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| K24 | Acetylation | Uniprot | |
| K24 | Ubiquitination | Uniprot | |
| T32 | Phosphorylation | Uniprot | |
| T43 | Phosphorylation | Uniprot | |
| R49 | Methylation | Uniprot | |
| S54 | Phosphorylation | Uniprot | |
| R58 | Methylation | Uniprot | |
| Y61 | Phosphorylation | Uniprot | |
| K64 | Ubiquitination | Uniprot | |
| K69 | Ubiquitination | Uniprot | |
| K92 | Acetylation | Uniprot | |
| K92 | Ubiquitination | Uniprot | |
| K97 | Ubiquitination | Uniprot | |
| K115 | Ubiquitination | Uniprot | |
| S121 | Phosphorylation | Uniprot | |
| T131 | Phosphorylation | Uniprot | |
| K148 | Ubiquitination | Uniprot | |
| K153 | Ubiquitination | Uniprot | |
| K201 | Ubiquitination | Uniprot | |
| K212 | Ubiquitination | Uniprot | |
| K219 | Acetylation | Uniprot | |
| S265 | Phosphorylation | Uniprot | |
| K273 | Ubiquitination | Uniprot | |
| T277 | Phosphorylation | Uniprot | |
| K291 | Ubiquitination | Uniprot | |
| S292 | Phosphorylation | Uniprot | |
| S295 | Phosphorylation | Uniprot | |
| S299 | Phosphorylation | Uniprot | |
| K303 | Ubiquitination | Uniprot | |
| T352 | Phosphorylation | Uniprot | |
| S365 | Phosphorylation | Uniprot | |
| S367 | Phosphorylation | Uniprot | |
| S369 | Phosphorylation | Uniprot | |
| Y378 | Phosphorylation | Uniprot | |
| K390 | Ubiquitination | Uniprot | |
| Y414 | Phosphorylation | Uniprot | |
| Y416 | Phosphorylation | Uniprot | |
| K420 | Ubiquitination | Uniprot | |
| K466 | Ubiquitination | Uniprot | |
| R470 | Methylation | Uniprot | |
| T475 | Phosphorylation | Uniprot | |
| Y479 | Phosphorylation | Uniprot | |
| S482 | Phosphorylation | Uniprot | |
| K489 | Acetylation | Uniprot | |
| K489 | Ubiquitination | Uniprot | |
| T491 | Phosphorylation | Uniprot | |
| Y492 | Phosphorylation | Uniprot | |
| K496 | Methylation | Uniprot | |
| K496 | Ubiquitination | Uniprot |
研究背景
Involved in pre-mRNA splicing as a component of the splicing factor SF3A complex that contributes to the assembly of the 17S U2 snRNP, and the subsequent assembly of the pre-spliceosome 'E' complex and the pre-catalytic spliceosome 'A' complex. Involved in pre-mRNA splicing as a component of pre-catalytic spliceosome 'B' complexes.
Nucleus speckle. Nucleus.
Ubiquitous.
Component of splicing factor SF3A which is composed of three subunits; SF3A3/SAP61, SF3A2/SAP62 and SF3A1/SAP114. SF3A1 functions as scaffold that interacts directly with both SF3A2 and SF3A3. SF3A associates with the splicing factor SF3B and a 12S RNA unit to form the mature 17S U2 small nuclear ribonucleoprotein complex (17S U2 snRNP). Identified in the spliceosome 'E' complex, a precursor of the spliceosome 'A' complex. Identified in the spliceosome 'A' and 'B' complexes. Identified in the spliceosome 'C' complex.
Belongs to the SF3A3 family.
研究领域
· Genetic Information Processing > Transcription > Spliceosome.
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