产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF2253, RRID:AB_2839482.
展开/折叠
RNA polymerase II TBP-associated factor subunit F; TAF 7; TAF(II)55; TAF2F; TAF7; TAF7 RNA polymerase II; TAF7 RNA polymerase II TATA box binding protein (TBP) associated factor 55 kDa; TAF7_HUMAN; TAFII 55; TAFII-55; TAFII55; TATA box binding protein (TBP) associated factor RNA polymerase II F 55kD; TATA box binding protein associated factor 2F; TBP associated factor F; Transcription factor IID subunit TAFII55; Transcription initiation factor TFIID 55 kDa subunit; Transcription initiation factor TFIID subunit 7;
抗原和靶标
- Q15545 TAF7_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSKSKDDAPHELESQFILRLPPEYASTVRRAVQSGHVNLKDRLTIELHPDGRHGIVRVDRVPLASKLVDLPCVMESLKTIDKKTFYKTADICQMLVSTVDGDLYPPVEEPVASTDPKASKKKDKDKEKKFIWNHGITLPLKNVRKRRFRKTAKKKYIESPDVEKEVKRLLSTDAEAVSTRWEIIAEDETKEAENQGLDISSPGMSGHRQGHDSLEHDELREIFNDLSSSSEDEDETQHQDEEDINIIDTEEDLERQLQDKLNESDEQHQENEGTNQLVMGIQKQIDNMKGKLQETQDRAKRQEDLIMKVENLALKNRFQAVLDELKQKEDREKEQLSSLQEELESLLEK
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q15545 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K5 | Methylation | Uniprot | |
| K5 | Ubiquitination | Uniprot | |
| S34 | Phosphorylation | Uniprot | |
| K40 | Ubiquitination | Uniprot | |
| K66 | Ubiquitination | Uniprot | |
| K129 | Sumoylation | Uniprot | |
| K141 | Ubiquitination | Uniprot | |
| K155 | Ubiquitination | Uniprot | |
| Y156 | Phosphorylation | Uniprot | |
| S159 | Phosphorylation | Uniprot | |
| S171 | Phosphorylation | Uniprot | |
| T172 | Phosphorylation | Uniprot | |
| S200 | Phosphorylation | Uniprot | |
| S201 | Phosphorylation | Uniprot | |
| S205 | Phosphorylation | Uniprot | |
| S213 | Phosphorylation | Uniprot | |
| S227 | Phosphorylation | Uniprot | |
| S228 | Phosphorylation | Uniprot | |
| S229 | Phosphorylation | Uniprot | |
| S230 | Phosphorylation | Uniprot | |
| S264 | Phosphorylation | P21675 (TAF1) | Uniprot |
| T274 | Phosphorylation | Uniprot | |
| K291 | Acetylation | Uniprot |
研究背景
Functions as a component of the DNA-binding general transcription factor complex TFIID, a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. Present in both of the previously described TFIID species which either lack or contain TAFII30 (TFIID alpha and TFIID beta respectively).
Phosphorylated by CIITA. Phosphorylation at Ser-264 by TAF1 in early G1 phase disrupts binding to TAF1.
Nucleus.
Ubiquitous.
TFIID is composed of TATA binding protein (TBP) and a number of TBP-associated factors (TAFs). Part of a TFIID-containing RNA polymerase II pre-initiation complex that is composed of TBP and at least GTF2A1, GTF2A2, GTF2E1, GTF2E2, GTF2F1, GTF2H2, GTF2H3, GTF2H4, GTF2H5, GTF2B, TCEA1, ERCC2, ERCC3, TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13. Interacts with TAF1; the interaction is direct. Interacts with TAFII250, TAFII100, TAFII28, TAFII20, and TAFII18, but not with TAFII30 or TBP. Component of some MLL1/MLL complex, at least composed of the core components KMT2A/MLL1, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components BAP18, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, MYST1/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Binds to CIITA and TAF1 and inhibits their acetyltransferase activity, thereby repressing activated transcription and behaving as a repressor at these promoters.
The [KR]-[STA]-K motif is specifically recognized by the SETD7 methyltransferase, which methylates Lys-5 in vitro.
Belongs to the TAF7 family.
研究领域
· Genetic Information Processing > Transcription > Basal transcription factors.
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