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| 产品: | USP1 抗体 |
| 货号: | DF2257 |
| 描述: | Rabbit polyclonal antibody to USP1 |
| 应用: | WB IHC |
| 反应: | Human |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
| 分子量: | 88 kDa; 88kD(Calculated). |
| 蛋白号: | O94782 |
| RRID: | AB_2839486 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF2257, RRID:AB_2839486.
展开/折叠
Deubiquitinating enzyme 1; hUBP; Ubiquitin carboxyl terminal hydrolase 1; Ubiquitin carboxyl-terminal hydrolase 1; Ubiquitin specific peptidase 1; Ubiquitin specific protease 1; Ubiquitin thioesterase 1; Ubiquitin thiolesterase 1; Ubiquitin-specific-processing protease 1; UBP; UBP1_HUMAN; USP 1; Usp1;
抗原和靶标
- O94782 UBP1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPGVIPSESNGLSRGSPSKKNRLSLKFFQKKETKRALDFTDSQENEEKASEYRASEIDQVVPAAQSSPINCEKRENLLPFVGLNNLGNTCYLNSILQVLYFCPGFKSGVKHLFNIISRKKEALKDEANQKDKGNCKEDSLASYELICSLQSLIISVEQLQASFLLNPEKYTDELATQPRRLLNTLRELNPMYEGYLQHDAQEVLQCILGNIQETCQLLKKEEVKNVAELPTKVEEIPHPKEEMNGINSIEMDSMRHSEDFKEKLPKGNGKRKSDTEFGNMKKKVKLSKEHQSLEENQRQTRSKRKATSDTLESPPKIIPKYISENESPRPSQKKSRVKINWLKSATKQPSILSKFCSLGKITTNQGVKGQSKENECDPEEDLGKCESDNTTNGCGLESPGNTVTPVNVNEVKPINKGEEQIGFELVEKLFQGQLVLRTRCLECESLTERREDFQDISVPVQEDELSKVEESSEISPEPKTEMKTLRWAISQFASVERIVGEDKYFCENCHHYTEAERSLLFDKMPEVITIHLKCFAASGLEFDCYGGGLSKINTPLLTPLKLSLEEWSTKPTNDSYGLFAVVMHSGITISSGHYTASVKVTDLNSLELDKGNFVVDQMCEIGKPEPLNEEEARGVVENYNDEEVSIRVGGNTQPSKVLNKKNVEAIGLLGGQKSKADYELYNKASNPDKVASTAFAENRNSETSDTTGTHESDRNKESSDQTGINISGFENKISYVVQSLKEYEGKWLLFDDSEVKVTEEKDFLNSLSPSTSPTSTPYLLFYKKL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O94782 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S7 | Phosphorylation | Uniprot | |
| S13 | Phosphorylation | Uniprot | |
| S16 | Phosphorylation | Uniprot | |
| T33 | Phosphorylation | Uniprot | |
| S42 | Phosphorylation | Uniprot | |
| K48 | Ubiquitination | Uniprot | |
| S67 | Phosphorylation | Uniprot | |
| K73 | Ubiquitination | Uniprot | |
| K110 | Ubiquitination | Uniprot | |
| K124 | Sumoylation | Uniprot | |
| K130 | Acetylation | Uniprot | |
| K224 | Ubiquitination | Uniprot | |
| K232 | Sumoylation | Uniprot | |
| K232 | Ubiquitination | Uniprot | |
| S248 | Phosphorylation | Uniprot | |
| S273 | Phosphorylation | Uniprot | |
| K283 | Ubiquitination | Uniprot | |
| K285 | Ubiquitination | Uniprot | |
| T307 | Phosphorylation | Uniprot | |
| S308 | Phosphorylation | Uniprot | |
| T310 | Phosphorylation | Uniprot | |
| S313 | Phosphorylation | P06493 (CDK1) | Uniprot |
| S323 | Phosphorylation | Uniprot | |
| S327 | Phosphorylation | Uniprot | |
| K343 | Ubiquitination | Uniprot | |
| S350 | Phosphorylation | Uniprot | |
| K360 | Ubiquitination | Uniprot | |
| K368 | Ubiquitination | Uniprot | |
| S398 | Phosphorylation | Uniprot | |
| S475 | Phosphorylation | Uniprot | |
| K483 | Ubiquitination | Uniprot | |
| K503 | Ubiquitination | Uniprot | |
| K523 | Ubiquitination | Uniprot | |
| T529 | Phosphorylation | Uniprot | |
| K610 | Ubiquitination | Uniprot | |
| K623 | Ubiquitination | Uniprot | |
| K656 | Ubiquitination | Uniprot | |
| K675 | Ubiquitination | Uniprot | |
| Y678 | Phosphorylation | Uniprot | |
| Y681 | Phosphorylation | Uniprot | |
| K683 | Ubiquitination | Uniprot | |
| K689 | Ubiquitination | Uniprot | |
| K716 | Acetylation | Uniprot | |
| K716 | Ubiquitination | Uniprot | |
| S727 | Phosphorylation | Uniprot | |
| K732 | Ubiquitination | Uniprot | |
| K741 | Ubiquitination | Uniprot | |
| K746 | Ubiquitination | Uniprot | |
| K761 | Ubiquitination | Uniprot | |
| S768 | Phosphorylation | Uniprot | |
| S770 | Phosphorylation | Uniprot | |
| S772 | Phosphorylation | Uniprot | |
| T774 | Phosphorylation | Uniprot | |
| S775 | Phosphorylation | Uniprot |
研究背景
Negative regulator of DNA damage repair which specifically deubiquitinates monoubiquitinated FANCD2. Also involved in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA. Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity.
Autocatalytic cleavage of USP1 following UV irradiation inactivates it leading to an increase in ubiquitinated PCNA, recruitment of POLH and translesion synthesis.
Ubiquitinated; leading to its subsequent proteasomal degradation.
Nucleus.
Interacts with FANCD2 and PCNA. Interacts with WDR48.
Belongs to the peptidase C19 family.
研究领域
· Genetic Information Processing > Replication and repair > Fanconi anemia pathway.
文献引用
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