ASPP2 Antibody - #DF2275

>>买2送1 进行中>>
>>满百送 进行中>>
>>赠送JD卡 进行中>>
>>3倍积分 进行中>>
(1)  
产品: ASPP2 抗体
货号: DF2275
描述: Rabbit polyclonal antibody to ASPP2
应用: WB
反应: Human, Mouse
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 170 kDa; 126kD(Calculated).
蛋白号: Q13625
RRID: AB_2839504

浏览相似产品>>

   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

联系销售
相关下载
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
ASPP2 Antibody detects endogenous levels of total ASPP2.
RRID:
AB_2839504
引用格式: Affinity Biosciences Cat# DF2275, RRID:AB_2839504.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

53BP2; Apoptosis stimulating of p53 protein 2; Apoptosis stimulating protein of p53 2; Apoptosis-stimulating of p53 protein 2; ASPP2; ASPP2_HUMAN; BBP; Bcl2 binding protein; Bcl2-binding protein; NY REN 51 antigen; p53 binding protein 2; p53-binding protein 2; p53BP2; PPP1R13A; Renal carcinoma antigen NY-REN-51; Tp53bp2; Tumor protein p53 binding protein 2; Tumor suppressor p53 binding protein 2; Tumor suppressor p53-binding protein 2;

抗原和靶标

免疫原:
Uniprot:

Q13625(ASPP2_HUMAN) >>Visit HPA database.

基因/基因ID:
TP53BP2(7159)
表达:
Q13625 ASPP2_HUMAN:

Widely expressed. Expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocyte. Reduced expression in breast carcinomas expressing a wild-type TP53 protein. Overexpressed in lung cancer cell lines.

描述:
Regulator that plays a central role in regulation of apoptosis and cell growth via its interactions. Regulates TP53 by enhancing the DNA binding and transactivation function of TP53 on the promoters of proapoptotic genes in vivo. Inhibits the ability of APPBP1 to conjugate NEDD8 to CUL1, and thereby decreases APPBP1 ability to induce apoptosis. Impedes cell cycle progression at G2/M. Its apoptosis-stimulating activity is inhibited by its interaction with DDX42
序列:
MMPMFLTVYLSNNEQHFTEVPVTPETICRDVVDLCKEPGESDCHLAEVWCGSERPVADNERMFDVLQRFGSQRNEVRFFLRHERPPGRDIVSGPRSQDPSLKRNGVKVPGEYRRKENGVNSPRMDLTLAELQEMASRQQQQIEAQQQLLATKEQRLKFLKQQDQRQQQQVAEQEKLKRLKEIAENQEAKLKKVRALKGHVEQKRLSNGKLVEEIEQMNNLFQQKQRELVLAVSKVEELTRQLEMLKNGRIDSHHDNQSAVAELDRLYKELQLRNKLNQEQNAKLQQQRECLNKRNSEVAVMDKRVNELRDRLWKKKAALQQKENLPVSSDGNLPQQAASAPSRVAAVGPYIQSSTMPRMPSRPELLVKPALPDGSLVIQASEGPMKIQTLPNMRSGAASQTKGSKIHPVGPDWSPSNADLFPSQGSASVPQSTGNALDQVDDGEVPLREKEKKVRPFSMFDAVDQSNAPPSFGTLRKNQSSEDILRDAQVANKNVAKVPPPVPTKPKQINLPYFGQTNQPPSDIKPDGSSQQLSTVVPSMGTKPKPAGQQPRVLLSPSIPSVGQDQTLSPGSKQESPPAAAVRPFTPQPSKDTLLPPFRKPQTVAASSIYSMYTQQQAPGKNFQQAVQSALTKTHTRGPHFSSVYGKPVIAAAQNQQQHPENIYSNSQGKPGSPEPETEPVSSVQENHENERIPRPLSPTKLLPFLSNPYRNQSDADLEALRKKLSNAPRPLKKRSSITEPEGPNGPNIQKLLYQRTTIAAMETISVPSYPSKSASVTASSESPVEIQNPYLHVEPEKEVVSLVPESLSPEDVGNASTENSDMPAPSPGLDYEPEGVPDNSPNLQNNPEEPNPEAPHVLDVYLEEYPPYPPPPYPSGEPEGPGEDSVSMRPPEITGQVSLPPGKRTNLRKTGSERIAHGMRVKFNPLALLLDSSLEGEFDLVQRIIYEVDDPSLPNDEGITALHNAVCAGHTEIVKFLVQFGVNVNAADSDGWTPLHCAASCNNVQVCKFLVESGAAVFAMTYSDMQTAADKCEEMEEGYTQCSQFLYGVQEKMGIMNKGVIYALWDYEPQNDDELPMKEGDCMTIIHREDEDEIEWWWARLNDKEGYVPRNLLGLYPRIKPRQRSLA

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q13625 作为底物

Site PTM Type Enzyme
S71 Phosphorylation
S92 Phosphorylation
S100 Phosphorylation
K102 Ubiquitination
S121 Phosphorylation
K152 Ubiquitination
K160 Ubiquitination
S206 Phosphorylation
K224 Ubiquitination
K234 Ubiquitination
K246 Ubiquitination
R265 Methylation
K268 Ubiquitination
K275 Ubiquitination
K283 Ubiquitination
S296 Phosphorylation
K303 Ubiquitination
K322 Ubiquitination
Y350 Phosphorylation
S353 Phosphorylation
T355 Phosphorylation
S361 Phosphorylation
T389 Phosphorylation
S414 Phosphorylation
S458 Phosphorylation
S471 Phosphorylation
T474 Phosphorylation
S480 Phosphorylation
S481 Phosphorylation
K493 Ubiquitination
S556 Phosphorylation
S558 Phosphorylation
S569 Phosphorylation
S572 Phosphorylation
S576 Phosphorylation
T586 Phosphorylation P28482 (MAPK1)
T603 Phosphorylation
S607 Phosphorylation
Y610 Phosphorylation
S611 Phosphorylation
Y613 Phosphorylation
Y645 Phosphorylation
Y664 Phosphorylation
S665 Phosphorylation
S667 Phosphorylation
S673 Phosphorylation
S698 Phosphorylation
T700 Phosphorylation
Y710 Phosphorylation
S714 Phosphorylation
K724 Ubiquitination
S736 Phosphorylation
S737 Phosphorylation
T739 Phosphorylation
S783 Phosphorylation
Y791 Phosphorylation
S827 Phosphorylation P28482 (MAPK1)
Y869 Phosphorylation
Y874 Phosphorylation
Y947 Phosphorylation

研究背景

功能:

Regulator that plays a central role in regulation of apoptosis and cell growth via its interactions with proteins such as TP53. Regulates TP53 by enhancing the DNA binding and transactivation function of TP53 on the promoters of proapoptotic genes in vivo. Inhibits the ability of APPBP1 to conjugate NEDD8 to CUL1, and thereby decreases APPBP1 ability to induce apoptosis. Impedes cell cycle progression at G2/M. Its apoptosis-stimulating activity is inhibited by its interaction with DDX42.

细胞定位:

Cytoplasm>Perinuclear region. Nucleus.
Note: Predominantly found in the perinuclear region. Some small fraction is nuclear. Sequester in the cytoplasm on overexpression of DDX42.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Widely expressed. Expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocyte. Reduced expression in breast carcinomas expressing a wild-type TP53 protein. Overexpressed in lung cancer cell lines.

亚基结构:

Interacts with P53/TP53; the interaction promotes pro-apototic activity. Interacts with BCL2. Interacts with protein phosphatase 1. Interacts with RELA NF-kappa-B subunit. This interaction probably prevents the activation of apoptosis, possibly by preventing its interaction with TP53. Interacts with APC2 and APPBP1. Interacts with DDX42 (via the C-terminus); the interaction is not inhibited by TP53BP2 ubiquitination and is independent of p53/TP53.

蛋白家族:

The ankyrin repeats and the SH3 domain are required for a specific interactions with TP53.

Belongs to the ASPP family.

研究领域

· Environmental Information Processing > Signal transduction > Hippo signaling pathway.   (View pathway)

限制条款

产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）

产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.